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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1560 [new locus tag: SA_RS08805 ]
  • pan locus tag?: SAUPAN004406000
  • symbol: SA1560
  • pan gene symbol?: facZ
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1560 [new locus tag: SA_RS08805 ]
  • symbol: SA1560
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1788940..1789431
  • length: 492
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGATTGGATTTTACCAATTGCTGGAATTATCGCTGCGATTGCATTCTTAATTTTATGT
    ATCGGTATCGTAGCTGTATTAAATTCTGTTAAGAAAAACTTAGATTATGTTGCAAAAACA
    CTTGACGGTGTAGAAGGTCAAGTTCAAGGTATTACTCGTGAAACAACAGATTTACTTCAT
    AAAGTAAACCGTTTAACTGAGGATATCCAAGGTAAAGTAGATCGTTTAAACTCAGTTGTA
    GATGCTGTTAAAGGTATCGGTGACTCAGTACAAACGTTAAACAGCTCTGTAGATCGTGTA
    ACAAATTCAATTACACATAATATTTCTCAAAATGAAGATAAAATCTCACAAGTTGTTCAA
    TGGTCAAATGTTGCAATGGAAATTGCAGACAAATGGCAAAATAGACACTACCGTCGTGGA
    AGTGCAAATTACAAAGCTAATAATGTAGCAACTGATGCAAATCATAGCTATACTTCTAGA
    GTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    492

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1560 [new locus tag: SA_RS08805 ]
  • symbol: SA1560
  • description: hypothetical protein
  • length: 163
  • theoretical pI: 7.67054
  • theoretical MW: 18002.2
  • GRAVY: -0.220245

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Pathogenesis virulence factor Mce family protein (TIGR00996; HMM-score: 33)
    and 7 more
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 14.3)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 14.3)
    helix-rich protein (TIGR04523; HMM-score: 14.1)
    Metabolism Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 13.5)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 13.5)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 13.5)
    two transmembrane protein (TIGR04527; HMM-score: 10.8)
  • TheSEED  :
    • UPF0478 protein YtxG
  • ⊞PFAM:
    no clan defined DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 103.1)
    and 44 more
    TPR (CL0020) COG6_N; Conserved oligomeric complex COG6, N-terminal (PF06419; HMM-score: 26.9)
    no clan defined DUF7310; Coiled-coil region of unknown function (DUF7310) (PF23991; HMM-score: 26)
    Baculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 22.5)
    Concanavalin (CL0004) Laminin_II; Laminin Domain II (PF06009; HMM-score: 21.3)
    no clan defined Focal_AT; Focal adhesion targeting region (PF03623; HMM-score: 19.6)
    HsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 18.5)
    TPR (CL0020) Sec10_N; Exocyst complex component Sec10, N-terminal (PF20667; HMM-score: 17.9)
    NUDIX (CL0261) NUDIX_5; NUDIX, or N-terminal NPxY motif-rich, region of KRIT (PF16705; HMM-score: 17.7)
    no clan defined Gp58; gp58-like protein (PF07902; HMM-score: 16.8)
    Chlorosome_CsmC; Chlorosome envelope protein C (PF11098; HMM-score: 16.6)
    BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 16.3)
    FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 16.1)
    Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 16.1)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 16)
    MCPsignal; Methyl-accepting chemotaxis protein (MCP) signalling domain (PF00015; HMM-score: 15.9)
    BORCS6; BLOC-1-related complex sub-unit 6 C-terminal helix (PF10157; HMM-score: 15.8)
    NPV_P10; Nucleopolyhedrovirus P10 protein (PF05531; HMM-score: 15.7)
    TPR (CL0020) COG3_N; Conserved oligomeric Golgi complex subunit 3, N-terminal (PF04136; HMM-score: 15.6)
    no clan defined Tweety; Tweety (PF04906; HMM-score: 15.6)
    Ferritin (CL0044) DUF2383; Domain of unknown function (DUF2383) (PF09537; HMM-score: 15.6)
    STAND_N (CL0587) SesA; N-terminal domain on NACHT_NTPase and P-loop NTPases (PF17107; HMM-score: 15.4)
    no clan defined BORCS8; BLOC-1-related complex sub-unit 8 (PF10167; HMM-score: 14.6)
    DUF7217; Domain of unknown function (DUF7217) (PF23854; HMM-score: 14.6)
    Fzo_mitofusin; fzo-like conserved region (PF04799; HMM-score: 14.4)
    UPF0184; Uncharacterised protein family (UPF0184) (PF03670; HMM-score: 14.3)
    DUF16; Protein of unknown function DUF16 (PF01519; HMM-score: 14.2)
    Prominin; Prominin (PF05478; HMM-score: 14.1)
    Spectrin (CL0743) Spectrin_Anc-1; Nuclear anchorage protein 1, spectrin-like repeat (PF24611; HMM-score: 14)
    no clan defined Laminin_I; Laminin Domain I (PF06008; HMM-score: 13.9)
    PDDEXK (CL0236) RmuC; RmuC family (PF02646; HMM-score: 13.8)
    Apolipoprotein (CL0725) ApoLp-III; Apolipophorin-III precursor (apoLp-III) (PF07464; HMM-score: 13.8)
    no clan defined BORCS7; BLOC-1-related complex sub-unit 7 (PF16088; HMM-score: 13.6)
    TPR (CL0020) COG4_N; Conserved oligomeric Golgi complex subunit 4, N-terminal (PF20663; HMM-score: 13.4)
    no clan defined AI-2E_transport; AI-2E family transporter (PF01594; HMM-score: 13.2)
    TPR (CL0020) COG5_N; Conserved oligomeric Golgi complex subunit 5, N-terminal (PF10392; HMM-score: 13.2)
    no clan defined DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 12.9)
    TPR (CL0020) EXOC6_Sec15_N; Exocyst complex component EXOC6/Sec15, N-terminal (PF20651; HMM-score: 12.8)
    no clan defined DUF3450; Protein of unknown function (DUF3450) (PF11932; HMM-score: 12.7)
    DUF6674; Family of unknown function (DUF6674) (PF20379; HMM-score: 12.7)
    DivIVA; DivIVA protein (PF05103; HMM-score: 12.4)
    Cluap1; Clusterin-associated protein-1 (PF10234; HMM-score: 12.3)
    SNARE-fusion (CL0445) Syntaxin_2; Syntaxin-like protein (PF14523; HMM-score: 12.1)
    E-set (CL0159) DUF4179; Domain of unknown function (DUF4179) (PF13786; HMM-score: 11.2)
    no clan defined GP41; Retroviral envelope protein (PF00517; HMM-score: 8.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0103
    • Cytoplasmic Membrane Score: 0.9886
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0009
  • ⊞LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.089509
    • TAT(Tat/SPI): 0.00082
    • LIPO(Sec/SPII): 0.071432
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGVEGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTNSITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSRVDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • ⊟interaction partners:
    SA1409(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SA2047(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SA1245(sucA)2-oxoglutarate dehydrogenase E1  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jump up to: 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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