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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0765 [new locus tag: SACOL_RS03930 ]
  • pan locus tag?: SAUPAN002590000
  • symbol: saeS
  • pan gene symbol?: saeS
  • synonym:
  • product: sensor histidine kinase SaeS

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0765 [new locus tag: SACOL_RS03930 ]
  • symbol: saeS
  • product: sensor histidine kinase SaeS
  • replicon: chromosome
  • strand: -
  • coordinates: 787731..788786
  • length: 1056
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTGTTATCAATTAGAAGTCAAATCATTATTGGCGTCGTATCGAGTATACTATTAACT
    TCAACTATTTTAGCAATTGCATATATTTTAATGTGGTTTAACGGCCATATGACACTAACT
    TTGACCTTAACGACAATAATTACAAGCTGTTTAACCTTATTAATATGTAGTATTTTTATT
    AATCCACTTATACAAAAAATTAAGCAGTTTAATATAAAAACTAAGCAATTTGCTAACGGA
    AATTACGCAAGCAATGATAAAACGTTTAATTCACCAAAAGAAATTTATGAATTAAATCAA
    TCTTTTAATAAAATGGCTTCTGAAATTACGCAACAAATGAATCAAATTAAATCCGAACAA
    CAAGAAAAAACAGAACTGATTCAAAACTTAGCCCATGATTTAAAAACACCTTTAGCAAGC
    ATTATTTCATATTCTGAAGGACTACGTGATGGTATAATCACTAAGGATCATGAGATTAAA
    GAGTCATACGACATATTAATTAAACAAGCAAACAGATTATCAACATTATTTGATGATATG
    ACTCATATTATCACTTTAAATACAGGTAAAACATATCCCCCAGAATTAATACAACTAGAC
    CAATTACTTGTATCAATATTGCAACCATATGAGCAACGTATCAAACATGAAAACCGCACA
    TTAGAAGTGAATTTCTGTAACGAAATTGATGCATTTTATCAATATCGAACGCCACTTGAG
    CGTATTTTAACAAACTTACTTGATAATGCGCTAAAATTTTCAAATGTTGGTAGTCGCATT
    GATATTAATATTAGTGAAAACGAAGATCAAGATACTATCGACATTGCTATTAGCGATGAA
    GGTATTGGCATTATACCAGAACTACAAGAACGTATATTCGAACGTACATTCAGAGTAGAA
    AACTCTCGTAATACAAAAACGGGTGGTTCTGGATTAGGCTTATATATAGCTAATGAACTC
    GCGCAACAAAATAACGCAAAAATCAGTGTAAGCAGTGATATAGATGTAGGAACTACGATG
    ACTGTAACATTACACAAATTAGACATTACGTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0765 [new locus tag: SACOL_RS03930 ]
  • symbol: SaeS
  • description: sensor histidine kinase SaeS
  • length: 351
  • theoretical pI: 4.97145
  • theoretical MW: 39741.2
  • GRAVY: -0.132764

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • ⊞TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 140.7)
    and 7 more
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 106.6)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 85.9)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 64)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
  • TheSEED  :
    • Two component system histidine kinase (EC 2.7.3.-)
  • ⊞PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 92.6)
    and 13 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 50.4)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 26)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.9)
    OB (CL0021) HIN; HIN-200/IF120x domain (PF02760; HMM-score: 18.2)
    no clan defined Mis12; Mis12 protein (PF05859; HMM-score: 14.5)
    TACC (CL0679) Fungal_TACC; Fungal Transforming acidic coiled-coil (TACC) proteins (PF12709; HMM-score: 14)
    no clan defined Peptidase_M48_N; CAAX prenyl protease N-terminal, five membrane helices (PF16491; HMM-score: 14)
    DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 13.7)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 13.6)
    HAMP (CL0681) CovS-like_HAMP; Histidine kinase, HAMP domain (PF22610; HMM-score: 12.9)
    no clan defined DUF5856; Family of unknown function (DUF5856) (PF19174; HMM-score: 12.8)
    Nsp1_C; Nsp1-like C-terminal region (PF05064; HMM-score: 12)
    DUF7299; Family of unknown function (DUF7299) (PF23973; HMM-score: 10.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0003
    • Cytoplasmic Membrane Score: 0.9931
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0062
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003245
    • TAT(Tat/SPI): 0.000385
    • LIPO(Sec/SPII): 0.016327
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLTTIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSFNKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIKESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHENRTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIAISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDIDVGTTMTVTLHKLDITS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: SaeR (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Kathrin Rogasch, Vanessa Rühmling, Jan Pané-Farré, Dirk Höper, Christin Weinberg, Stephan Fuchs, Mareike Schmudde, Barbara M Bröker, Christiane Wolz, Michael Hecker, Susanne Engelmann
Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains.
J Bacteriol: 2006, 188(22);7742-58
[PubMed:17079681] [WorldCat.org] [DOI] (P p)Julia Schmitt, Insa Joost, Eric P Skaar, Mathias Herrmann, Markus Bischoff
Haemin represses the haemolytic activity of Staphylococcus aureus in an Sae-dependent manner.
Microbiology (Reading): 2012, 158(Pt 10);2619-2631
[PubMed:22859613] [WorldCat.org] [DOI] (I p)

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