PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000673
• pan locus tag^?: SAUPAN002590000
• symbol: saeS
• pan gene symbol^?: saeS
• synonym:
• product: two-component system sensor histidine kinase SaeS

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000673
• symbol: saeS
• product: two-component system sensor histidine kinase SaeS
• replicon: chromosome
• strand: -
• coordinates: 707813..708868
• length: 1056
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGGTGTTATCAATTAGAAGTCAAATCATTATTGGCGTCGTATCGAGTATACTATTAACT
  TCAACTATTTTAGCAATTGCATATATTTTAATGTGGTTTAACGGCCATATGACACTAACT
  TTGACCTTAACGACAATAATTACAAGCTGTTTAACCTTATTAATATGTAGTATTTTTATT
  AATCCACTTATACAAAAAATTAAGCAGTTTAATATAAAAACTAAGCAATTTGCTAACGGA
  AATTACGCAAGCAATGATAAAACGTTTAATTCACCAAAAGAAATTTATGAATTAAATCAA
  TCTTTTAATAAAATGGCTTCTGAAATTACGCAACAAATGAATCAAATTAAATCCGAACAA
  CAAGAAAAAACAGAACTGATTCAAAACTTAGCCCATGATTTAAAAACACCTTTAGCAAGC
  ATTATTTCATATTCTGAAGGACTACGTGATGGTATAATCACTAAGGATCATGAGATTAAA
  GAGTCATACGACATATTAATTAAACAAGCAAACAGATTATCAACATTATTTGATGATATG
  ACTCATATTATCACTTTAAATACAGGTAAAACATATCCCCCAGAATTAATACAACTAGAC
  CAATTACTTGTATCAATATTGCAACCATATGAGCAACGTATCAAACATGAAAACCGCACA
  TTAGAAGTGAATTTCTGTAGCGAAATTGATGCATTTTATCAATATCGAACGCCACTTGAG
  CGTATTTTAACAAACTTACTTGATAATGCGCTAAAATTTTCAAATGTTGGTAGTCGCATT
  GATATTAATATTAGTGAAAACAAAGATCAAGATACTATCGACATTGCTATTAGCGATGAA
  GGTATTGGCATTATACCAGAACTACAAGAACGTATATTCGAACGTACATTCAGAGTAGAA
  AACTCTCGTAATACAAAAACGGGTGGTTCTGGATTAGGCTTATATATAGCTAATGAACTC
  GCGCAACAAAATAACGCAAAAATCAGTGTAAGCAGTGATATAGATGTAGGAACTACGATG
  ACTGTAACATTACACAAATTAGACATTACGTCATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1056
  

⊟Protein

⊟General

• locus tag: JSNZ_000673
• symbol: SaeS
• description: two-component system sensor histidine kinase SaeS
• length: 351
• theoretical pI: 5.23652
• theoretical MW: 39713.2
• GRAVY: -0.126211

⊟Function

• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 140.5)
  and 7 more
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 106.8)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 85.5)
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 63.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.1)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.1)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.5)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.5)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 92)
  and 13 more
  HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 50.4)
  HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 25.9)
  His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.5)
  OB (CL0021) HIN; HIN-200/IF120x domain (PF02760; HMM-score: 18.3)
  no clan defined Mis12; Mis12 protein (PF05859; HMM-score: 14.5)
  TACC (CL0679) Fungal_TACC; Fungal Transforming acidic coiled-coil (TACC) proteins (PF12709; HMM-score: 14.2)
  no clan defined Peptidase_M48_N; CAAX prenyl protease N-terminal, five membrane helices (PF16491; HMM-score: 14)
  DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 13.7)
  His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 13.6)
  HAMP (CL0681) CovS-like_HAMP; Histidine kinase, HAMP domain (PF22610; HMM-score: 12.9)
  no clan defined DUF5856; Family of unknown function (DUF5856) (PF19174; HMM-score: 12.4)
  Nsp1_C; Nsp1-like C-terminal region (PF05064; HMM-score: 12)
  DUF7299; Family of unknown function (DUF7299) (PF23973; HMM-score: 10.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helices: 2
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0004
  • Cytoplasmic Membrane Score: 0.9924
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0069
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003245
  • TAT(Tat/SPI): 0.000385
  • LIPO(Sec/SPII): 0.016327
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLTTIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSFNKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIKESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHENRTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIAISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDIDVGTTMTVTLHKLDITS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications