From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00714
  • pan locus tag?: SAUPAN002590000
  • symbol: SAOUHSC_00714
  • pan gene symbol?: saeS
  • synonym:
  • product: sensor histidine kinase SaeS

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00714
  • symbol: SAOUHSC_00714
  • product: sensor histidine kinase SaeS
  • replicon: chromosome
  • strand: -
  • coordinates: 699194..700249
  • length: 1056
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTGTTATCAATTAGAAGTCAAATCATTATTGGCGTCGTATCGAGTATACTATTAACT
    TCAACTATTTTAGCAATTGCATATATTTTAATGTGGTTTAACGGCCATATGACACTAACT
    TTGACCTTAACGACAATAATTACAAGCTGTTTAACCTTATTAATATGTAGTATTTTTATT
    AATCCACTTATACAAAAAATTAAGCAGTTTAATATAAAAACTAAGCAATTTGCTAACGGA
    AATTACGCAAGCAATGATAAAACGTTTAATTCACCAAAAGAAATTTATGAATTAAATCAA
    TCTTTTAATAAAATGGCTTCTGAAATTACGCAACAAATGAATCAAATTAAATCCGAACAA
    CAAGAAAAAACAGAACTGATTCAAAACTTAGCCCATGATTTAAAAACACCTTTAGCAAGC
    ATTATTTCATATTCTGAAGGACTACGTGATGGTATAATCACTAAGGATCATGAGATTAAA
    GAGTCATACGACATATTAATTAAACAAGCAAACAGATTATCAACATTATTTGATGATATG
    ACTCATATTATCACTTTAAATACAGGTAAAACATATCCCCCAGAATTAATACAACTAGAC
    CAATTACTTGTATCAATATTGCAACCATATGAGCAACGTATCAAACATGAAAACCGCACA
    TTAGAAGTGAATTTCTGTAACGAAATTGATGCATTTTATCAATATCGAACGCCACTTGAG
    CGTATTTTAACAAACTTACTTGATAATGCGCTAAAATTTTCAAATGTTGGTAGTCGCATT
    GATATTAATATTAGTGAAAACGAAGATCAAGATACTATCGACATTGCTATTAGCGATGAA
    GGTATTGGCATTATACCAGAACTACAAGAACGTATATTCGAACGTACATTCAGAGTAGAA
    AACTCTCGTAATACAAAAACGGGTGGTTCTGGATTAGGCTTATATATAGCTAATGAACTC
    GCGCAACAAAATAACGCAAAAATCAGTGTAAGCAGTGATATAGATGTAGGAACTACGATG
    ACTGTAACATTACACAAATTAGACATTACGTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00714
  • symbol: SAOUHSC_00714
  • description: sensor histidine kinase SaeS
  • length: 351
  • theoretical pI: 4.97145
  • theoretical MW: 39741.2
  • GRAVY: -0.132764

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 140.7)
    and 7 more
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 106.6)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 85.9)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 64)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
  • TheSEED  :
    • Two component system histidine kinase (EC 2.7.3.-)
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 92.6)
    and 13 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 50.4)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 26)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.9)
    OB (CL0021) HIN; HIN-200/IF120x domain (PF02760; HMM-score: 18.2)
    no clan defined Mis12; Mis12 protein (PF05859; HMM-score: 14.5)
    TACC (CL0679) Fungal_TACC; Fungal Transforming acidic coiled-coil (TACC) proteins (PF12709; HMM-score: 14)
    no clan defined Peptidase_M48_N; CAAX prenyl protease N-terminal, five membrane helices (PF16491; HMM-score: 14)
    DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 13.7)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 13.6)
    HAMP (CL0681) CovS-like_HAMP; Histidine kinase, HAMP domain (PF22610; HMM-score: 12.9)
    no clan defined DUF5856; Family of unknown function (DUF5856) (PF19174; HMM-score: 12.8)
    Nsp1_C; Nsp1-like C-terminal region (PF05064; HMM-score: 12)
    DUF7299; Family of unknown function (DUF7299) (PF23973; HMM-score: 10.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0003
    • Cytoplasmic Membrane Score: 0.9931
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0062
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003245
    • TAT(Tat/SPI): 0.000385
    • LIPO(Sec/SPII): 0.016327
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLTTIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSFNKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIKESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHENRTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIAISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDIDVGTTMTVTLHKLDITS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR* (activation) regulon
    SaeR*(TF)important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 3.3 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

A T Giraudo, A Calzolari, A A Cataldi, C Bogni, R Nagel
The sae locus of Staphylococcus aureus encodes a two-component regulatory system.
FEMS Microbiol Lett: 1999, 177(1);15-22
[PubMed:10436918] [WorldCat.org] [DOI] (P p)C Goerke, U Fluckiger, A Steinhuber, W Zimmerli, C Wolz
Impact of the regulatory loci agr, sarA and sae of Staphylococcus aureus on the induction of alpha-toxin during device-related infection resolved by direct quantitative transcript analysis.
Mol Microbiol: 2001, 40(6);1439-47
[PubMed:11442841] [WorldCat.org] [DOI] (P p)Ana T Giraudo, Cecilia Mansilla, Ana Chan, Claudia Raspanti, Rosa Nagel
Studies on the expression of regulatory locus sae in Staphylococcus aureus.
Curr Microbiol: 2003, 46(4);246-50
[PubMed:12732971] [WorldCat.org] [DOI] (P p)Richard P Novick, Dunrong Jiang
The staphylococcal saeRS system coordinates environmental signals with agr quorum sensing.
Microbiology (Reading): 2003, 149(Pt 10);2709-2717
[PubMed:14523104] [WorldCat.org] [DOI] (P p)Andrea Steinhuber, Christiane Goerke, Manfred G Bayer, Gerd Döring, Christiane Wolz
Molecular architecture of the regulatory Locus sae of Staphylococcus aureus and its impact on expression of virulence factors.
J Bacteriol: 2003, 185(21);6278-86
[PubMed:14563862] [WorldCat.org] [DOI] (P p)Bret M Benton, J P Zhang, Skip Bond, Casey Pope, Todd Christian, Lawrence Lee, Kelly M Winterberg, Molly B Schmid, Jerry M Buysse
Large-scale identification of genes required for full virulence of Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8478-89
[PubMed:15576798] [WorldCat.org] [DOI] (P p)Niamh Harraghy, Jan Kormanec, Christiane Wolz, Dagmar Homerova, Christiane Goerke, Knut Ohlsen, Saara Qazi, Philip Hill, Mathias Herrmann
sae is essential for expression of the staphylococcal adhesins Eap and Emp.
Microbiology (Reading): 2005, 151(Pt 6);1789-1800
[PubMed:15941988] [WorldCat.org] [DOI] (P p)Yan Q Xiong, Julie Willard, Michael R Yeaman, Ambrose L Cheung, Arnold S Bayer
Regulation of Staphylococcus aureus alpha-toxin gene (hla) expression by agr, sarA, and sae in vitro and in experimental infective endocarditis.
J Infect Dis: 2006, 194(9);1267-75
[PubMed:17041853] [WorldCat.org] [DOI] (P p)A T Giraudo, C G Raspanti, A Calzolari, R Nagel
Characterization of a Tn551-mutant of Staphylococcus aureus defective in the production of several exoproteins.
Can J Microbiol: 1994, 40(8);677-81
[PubMed:7922890] [WorldCat.org] [DOI] (P p)A T Giraudo, H Rampone, A Calzolari, R Nagel
Phenotypic characterization and virulence of a sae- agr- mutant of Staphylococcus aureus.
Can J Microbiol: 1996, 42(2);120-3
[PubMed:8742355] [WorldCat.org] [DOI] (P p)A T Giraudo, A L Cheung, R Nagel
The sae locus of Staphylococcus aureus controls exoprotein synthesis at the transcriptional level.
Arch Microbiol: 1997, 168(1);53-8
[PubMed:9211714] [WorldCat.org] [DOI] (P p)

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_00714&oldid=96447"