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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08810 [old locus tag: SA1561 ]
  • pan locus tag?: SAUPAN004407000
  • symbol: SA_RS08810
  • pan gene symbol?: murC
  • synonym:
  • product: UDP-N-acetylmuramate--L-alanine ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08810 [old locus tag: SA1561 ]
  • symbol: SA_RS08810
  • product: UDP-N-acetylmuramate--L-alanine ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1789505..1790818
  • length: 1314
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1789505..1790818) NCBI
  • BioCyc: SA_RS08810 BioCyc
  • MicrobesOnline: see SA1561

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACACACTATCATTTTGTCGGAATTAAAGGTTCTGGCATGAGTTCATTAGCACAAATC
    ATGCATGATTTAGGACATGAAGTTCAAGGATCGGATATTGAGAACTACGTATTTACAGAA
    GTTGCTCTTAGAAATAAGGGGATAAAAATATTACCATTTGATGCTAATAACATAAAAGAA
    GATATGGTAGTTATACAAGGTAATGCATTCGCGAGTAGCCATGAAGAAATAGTACGTGCA
    CATCAATTGAAATTAGATGTTGTAAGTTATAATGATTTTTTAGGACAGATTATTGATCAA
    TATACTTCAGTAGCTGTAACTGGTGCACATGGTAAAACTTCTACAACAGGTTTATTATCA
    CATGTTATGAATGGTGATAAAAAGACTTCATTTTTAATTGGTGATGGCACAGGTATGGGA
    TTGCCTGAAAGTGATTATTTCGCTTTTGAGGCATGTGAATATAGACGTCACTTTTTAAGT
    TATAAACCTGATTACGCAATTATGACAAATATTGATTTCGATCATCCTGATTATTTTAAA
    GATATTAATGATGTTTTTGATGCATTCCAAGAAATGGCACATAATGTTAAAAAAGGTATT
    ATTGCTTGGGGTGATGATGAACATCTACGTAAAATTGAAGCAGATGTTCCAATTTATTAT
    TATGGATTTAAAGATTCGGATGACATTTATGCTCAAAATATTCAAATTACGGATAAAGGT
    ACTGCTTTTGATGTGTATGTGGATGGTGAGTTTTATGATCACTTCCTGTCTCCACAATAT
    GGTGACCATACAGTTTTAAATGCATTAGCTGTAATTGCGATTAGTTATTTAGAGAAGCTA
    GATGTTACAAATATTAAAGAAGCATTAGAAACGTTTGGTGGTGTTAAACGTCGTTTCAAT
    GAAACTACAATTGCAAATCAAGTTATTGTAGATGATTATGCACACCATCCAAGAGAAATT
    AGTGCTACAATTGAAACAGCACGAAAGAAATATCCACATAAAGAAGTTGTTGCAGTATTT
    CAACCACACACTTTCTCTAGAACACAAGCATTTTTAAATGAATTTGCAGAAAGTTTAAGT
    AAAGCAGATCGTGTATTCTTATGTGAAATTTTTGGATCAATTAGAGAAAATACTGGCGCA
    TTAACGATACAAGATTTAATTGATAAAATTGAAGGTGCATCGTTAATTAATGAAGATTCT
    ATTAATGTATTAGAACAATTTGATAATGCTGTTGTTTTATTTATGGGTGCAGGTGATATT
    CAAAAATTACAAAATGCATATTTAGATAAATTAGGCATGAAAAATGCGTTTTAA
    60
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    1314

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08810 [old locus tag: SA1561 ]
  • symbol: SA_RS08810
  • description: UDP-N-acetylmuramate--L-alanine ligase
  • length: 437
  • theoretical pI: 4.77031
  • theoretical MW: 49173.9
  • GRAVY: -0.222883

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.3.2.8?  ExPASy
    UDP-N-acetylmuramate--L-alanine ligase ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
  • ⊞TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 416.2)
    and 6 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 171.7)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 78.6)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 75.6)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 67.2)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 25.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 21.2)
  • TheSEED: see SA1561
  • ⊞PFAM:
    MurF-HprK_N (CL0365) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 85.2)
    P-loop_NTPase (CL0023) Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 84.6)
    and 4 more
    Mur_ligase_C_sf (CL0794) Mur_ligase_C; Mur ligase, glutamate ligase domain (PF02875; HMM-score: 62.2)
    P-loop_NTPase (CL0023) YqeC; YqeC protein (PF19842; HMM-score: 13.6)
    no clan defined DUF5751; Family of unknown function (DUF5751) (PF19025; HMM-score: 12.8)
    DUF6409; Family of unknown function (DUF6409) (PF19947; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9955
    • Cytoplasmic Membrane Score: 0.0006
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0036
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018125
    • TAT(Tat/SPI): 0.001102
    • LIPO(Sec/SPII): 0.001738
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALRNKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYTSVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLSYKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPIYYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISYLEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHKEVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEGASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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