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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04070 [old locus tag: SA0715 ]
  • pan locus tag?: SAUPAN002679000
  • symbol: SA_RS04070
  • pan gene symbol?: hprK
  • synonym:
  • product: HPr kinase/phosphorylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04070 [old locus tag: SA0715 ]
  • symbol: SA_RS04070
  • product: HPr kinase/phosphorylase
  • replicon: chromosome
  • strand: +
  • coordinates: 818444..819376
  • length: 933
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (818444..819376) NCBI
  • BioCyc: SA_RS04070 BioCyc
  • MicrobesOnline: see SA0715

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTAACGACAGAAAAACTAGTTGAAACATTAAAGTTAGATTTAATCGCTGGTGAAGAA
    GGACTATCGAAGCCAATTAAAAATGCTGATATATCAAGACCAGGCTTAGAGATGGCAGGT
    TATTTTTCACATTATGCGTCAGATAGAATACAACTATTAGGAACAACGGAACTATCGTTT
    TACAATTTATTACCAGATAAGGATCGCGCAGGTCGTATGCGTAAACTATGCAGACCAGAA
    ACGCCTGCAATTATTGTGACACGTGGATTGCAGCCACCAGAAGAATTAGTTGAAGCTGCA
    AAAGAATTAAATACCCCACTTATAGTTGCTAAAGATGCGACTACAAGTTTAATGAGTCGC
    TTAACAACGTTTTTAGAGCATGCACTTGCAAAGACGACATCTTTACATGGTGTTTTAGTA
    GATGTTTACGGTGTTGGTGTACTAATTACCGGTGATTCAGGAATAGGTAAAAGTGAGACT
    GCGTTGGAATTAGTTAAACGTGGGCATAGATTAGTAGCAGATGATAATGTAGAAATACGT
    CAAATTAATAAAGATGAACTAATAGGGAAACCACCAAAGTTAATAGAACATCTATTAGAA
    ATACGTGGACTAGGTATTATCAATGTTATGACTTTATTTGGCGCGGGTTCAATATTAACT
    GAAAAACGAATTAGATTAAATATTAATTTGGAAAACTGGAACAAGCAAAAGTTATATGAC
    CGCGTAGGTCTTAATGAAGAGACGCTAAGTATTTTAGATACTGAAATCACTAAAAAAACA
    ATACCTGTAAGACCTGGTAGAAATGTTGCGGTAATTATTGAGGTCGCTGCAATGAACTAT
    CGATTAAATATCATGGGCATTAACACTGCCGAAGAATTTAGTGAAAGATTAAATGAAGAA
    ATTATCAAGAACAGTCATAAGAGTGAGGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS04070 [old locus tag: SA0715 ]
  • symbol: SA_RS04070
  • description: HPr kinase/phosphorylase
  • length: 310
  • theoretical pI: 6.27773
  • theoretical MW: 34481.7
  • GRAVY: -0.173226

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.4.-?  ExPASy    EC 2.7.11.-?  ExPASy
    ATP + HPr = ADP + P-Ser-HPr? P-Ser-HPr + phosphate = HPr + diphosphate?
  • ⊞TIGRFAM:
    Signal transduction Regulatory functions Protein interactions HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
    Signal transduction Signal transduction PTS HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
    and 3 more
    HprK-related kinase B (TIGR04355; HMM-score: 23.5)
    HprK-related kinase A (TIGR04352; HMM-score: 15)
    SynChlorMet cassette protein ScmC (TIGR04249; HMM-score: 12.6)
  • TheSEED: see SA0715
  • ⊞PFAM:
    PEP-carboxyk (CL0374) Hpr_kinase_C; HPr Serine kinase C-terminal domain (PF07475; HMM-score: 250.7)
    and 7 more
    MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 135.7)
    no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 24.1)
    MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 21.6)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.5)
    AAA_18; AAA domain (PF13238; HMM-score: 13.1)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 12.7)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9675
    • Cytoplasmic Membrane Score: 0.0071
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0253
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00494
    • TAT(Tat/SPI): 0.000283
    • LIPO(Sec/SPII): 0.000768
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446880968 NCBI
  • RefSeq: WP_000958224 NCBI
  • UniProt: see SA0715

⊟Protein sequence[edit | edit source]

  • MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSHYASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKELNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSETALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSILTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVAAMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: data available for COL

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS04070&oldid=72338"
  • This page was last edited on 10 March 2016, at 21:40.
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