Navigation

  • Main page
  • Downloads
  • Getting Started
  • Recent changes
  • Random page
  • What links here
  • Related changes
  • Special pages
  • Printable version
  • Permanent link
  • Page information

personal-loginout

  • Log in
  • Not logged in
  • Talk
  • Contributions
  • Create account

Search

?

Navigation menu

Namespaces
  • Page
  • Discussion
English
From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Views
  • Read
  • Edit
  • History
  • Edit source

⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03070 [old locus tag: SA0528 ]
  • pan locus tag?: SAUPAN002347000
  • symbol: SA_RS03070
  • pan gene symbol?: hxlA
  • synonym:
  • product: 3-hexulose-6-phosphate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03070 [old locus tag: SA0528 ]
  • symbol: SA_RS03070
  • product: 3-hexulose-6-phosphate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 622952..623584
  • length: 633
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (622952..623584) NCBI
  • BioCyc: SA_RS03070 BioCyc
  • MicrobesOnline: see SA0528

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    GTGGAATTACAATTAGCAATTGATTTATTAAACAAAGAAGACGCGGCTGAGTTAGCAAAT
    AAAGTAAAAGATTATGTAGATATCGTAGAAATCGGTACGCCAATCATTTACAACGAAGGT
    TTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATG
    AAAATTATGGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTA
    ATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCAT
    AAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCA
    AAAGAACTAGATGAAATGGGTGCTGATTATATTGCAGTACACACTGGTTATGATTTACAA
    GCAGAAGGGCAATCACCATTAGAAAGTTTAAGAACCGTTAAATCTGTTATTAAAAATTCT
    AAAGTTGCAGTAGCAGGTGGAATTAAACCAGATACAATTAAAGATATTGTCGCTGAAAGT
    CCTGATCTTGTTATTGTTGGTGGCGGAATCGCAAATGCAGATGATCCAGTAGAAGCTGCA
    AAACAATGTCGCGCTGCAATCGAAGGTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03070 [old locus tag: SA0528 ]
  • symbol: SA_RS03070
  • description: 3-hexulose-6-phosphate synthase
  • length: 210
  • theoretical pI: 4.3222
  • theoretical MW: 22435.5
  • GRAVY: -0.0185714

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 4.1.2.43?  ExPASy
    3-hexulose-6-phosphate synthase D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde
  • ⊞TIGRFAM:
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 297.6)
    and 4 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotidine 5'-phosphate decarboxylase (TIGR01740; EC 4.1.1.23; HMM-score: 55.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 26.5)
    Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 21.6)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 11.4)
  • TheSEED: see SA0528
  • ⊞PFAM:
    TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 152.4)
    and 6 more
    Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 20.2)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 16.6)
    PRAI; N-(5'phosphoribosyl)anthranilate (PRA) isomerase (PF00697; HMM-score: 15.2)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 15.2)
    PcrB; PcrB family (PF01884; HMM-score: 14.1)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 9.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9406
    • Cytoplasmic Membrane Score: 0.003
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.056
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00232
    • TAT(Tat/SPI): 0.000099
    • LIPO(Sec/SPII): 0.000488
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446345006 NCBI
  • RefSeq: WP_000422861 NCBI
  • UniProt: see SA0528

⊟Protein sequence[edit | edit source]

  • MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAVKHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKNNKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNSKVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for COL, NCTC8325, Newman

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: data available for COL

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS03070&oldid=83441"
  • This page was last edited on 11 March 2016, at 03:58.
  • Privacy and Cookies
  • About AureoWiki
  • Imprint
We use Matomo for user statistics and cookies. Privacy and Cookies X
CancelTry again