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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS12080 [old locus tag: SACOL2296 ]
  • pan locus tag?: SAUPAN005775000
  • symbol: SACOL_RS12080
  • pan gene symbol?: —
  • synonym:
  • product: 2-hydroxyacid dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS12080 [old locus tag: SACOL2296 ]
  • symbol: SACOL_RS12080
  • product: 2-hydroxyacid dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2356914..2357867
  • length: 954
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2356914..2357867) NCBI
  • BioCyc: SACOL_RS12080 BioCyc
  • MicrobesOnline: see SACOL2296

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAAAAGTTTATGTTGCTGGTGCAATACCAGAAGTAGGTTTAAAACTTTTACAAGAA
    CATTTTGAAGTTGAAATGTATGAAGGTAAAGGATTAGTCGATAAAGACACTTTAATTAAA
    GGTGTTAAAAACGCGACTGCCTTAATTAGTTTATTATCTACAAACGTTGATAAAGATGTT
    ATCGATGCTGGTAAAGACTTAAAAATCATTGCCAACTATGGCGCTGGTTTTAATAATATT
    GATATCGAGTATGCCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACA
    AACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAA
    GGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGT
    AGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTA
    GCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAATCGCAAAGAA
    GAAAAAGAACGAGAAATCGGTGCAAAATATGTAGATTTAGATACACTATTAAAGAATGCA
    GATTTTATCACTATCAACGCTGCTTATAATCCTAAAATGCATCATTTAATTGATACAGAA
    CAATTTAAAATGATGAAATCTACGGCGTATTTAATCAATGCCTCTCGTGGTCCAATCGTG
    CACGAACAAGCACTAGTGCAAGCATTGAAAGATAATGAAATTGAAGGTGCTGCACTTGAT
    GTATATGAATTTGAACCAGATATTACCGATGACTTAAAATCACTTAATAATGTAGTATTA
    ACACCGCATATTGGTAATGCTACATTTGAAGCACGTGACATGATGTCTAAAATTGTTGCT
    AATGCTGCAATTAGTGCCGTTCAAGGTGAGAAACCACAATTTGTCGTTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS12080 [old locus tag: SACOL2296 ]
  • symbol: SACOL_RS12080
  • description: 2-hydroxyacid dehydrogenase
  • length: 317
  • theoretical pI: 4.91492
  • theoretical MW: 34674.3
  • GRAVY: -0.127445

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.-?  ExPASy
  • ⊞TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 268)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 17.5)
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 12.1)
  • TheSEED: see SACOL2296
  • ⊞PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 202.5)
    and 5 more
    2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 113.4)
    KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 20.3)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 20.1)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 16.1)
    AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9885
    • Cytoplasmic Membrane Score: 0.0008
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0104
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001313
    • TAT(Tat/SPI): 0.000138
    • LIPO(Sec/SPII): 0.000235
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2296
  • protein localization: see SACOL2296
  • quantitative data / protein copy number per cell: see SACOL2296
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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