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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07620 [old locus tag: SACOL1496 ]
  • pan locus tag?: SAUPAN003916000
  • symbol: SACOL_RS07620
  • pan gene symbol?: birA
  • synonym:
  • product: bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07620 [old locus tag: SACOL1496 ]
  • symbol: SACOL_RS07620
  • product: bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 1537306..1538277
  • length: 972
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1537306..1538277) NCBI
  • BioCyc: SACOL_RS07620 BioCyc
  • MicrobesOnline: see SACOL1496

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCAAAATATAGTCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATA
    TCTGGACAAAGCATTGCGGAATCACTTAATATTTCACGCACTGCAGTAAAAAAAGTGATT
    GACCAATTAAAGTTAGAGGGATGTAAAATAGATTCAGTAAATCATAAAGGGCATTTATTA
    CAACAGCTCCCAGATATTTGGTATCAAGGTATAATAGACCAATATACAAAAAGTTCTGCT
    TTGTTTGATTTTAGTGAAGTATACGATTCAATAGATTCTACACAACTTGCTGCGAAAAAG
    TCACTTGTTGGAAATCAATCTTCATTTTTTATCTTGAGTGATGAACAAACGAAAGGTCGT
    GGGCGATTTAATAGACATTGGAGTTCTTCAAAAGGGCAAGGACTTTGGATGTCTGTCGTG
    TTAAGACCTAACGTTGCATTCTCAATGATATCTAAATTTAATTTATTTATTGCATTAGGG
    ATAAGAGATGCGATTCAACATTTTAGTCAAGATGAAGTCAAAGTGAAATGGCCGAATGAT
    ATATATATTGATAATGGTAAAGTGTGTGGTTTCTTAACTGAAATGGTTGCTAATAATGAT
    GGTATAGAAGCAATAATATGTGGTATAGGTATTAATTTGACGCAACAACTAGAAAACTTT
    GATGAAAGTATTAGACATAGAGCAACAAGTATACAATTACATGATAAAAATAAATTAGAT
    AGATATCAATTTTTAGAGATATTACTTCAAGAAATTGAAAAAAGATATAATCAATTTTTA
    ACGTTACCTTTTTCTGAAATTCGTGAAGAATATACTGCAGCTTCTAATATTTGGAATAGA
    ACGTTGCTATTTACAGAAAATGATAAACAGTTTAAAGGACAAGCAATTGATTTAGATTAC
    GATGGCTATCTAATTGTTAGAGATGAAGCGGGTGAATCACACCGTTTAATTAGTGCAGAT
    ATAGATTTTTAA
    60
    120
    180
    240
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    360
    420
    480
    540
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    660
    720
    780
    840
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    960
    972

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS07620 [old locus tag: SACOL1496 ]
  • symbol: SACOL_RS07620
  • description: bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor
  • length: 323
  • theoretical pI: 5.92816
  • theoretical MW: 37086.7
  • GRAVY: -0.360681

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.3.4.15?  ExPASy
    Biotin--[acetyl-CoA-carboxylase] ligase ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair biotin--[acetyl-CoA-carboxylase] ligase (TIGR00121; EC 6.3.4.15; HMM-score: 180.1)
    and 4 more
    Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 32.2)
    Signal transduction Regulatory functions DNA interactions TyrR family helix-turn-helix domain (TIGR04381; HMM-score: 15.1)
    Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 12.5)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 11.7)
  • TheSEED: see SACOL1496
  • ⊞PFAM:
    tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 63.7)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 54.9)
    and 11 more
    SH3 (CL0010) BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 24.2)
    tRNA_synt_II (CL0040) BPL_LplA_LipB_2; Biotin/lipoate A/B protein ligase family (PF16917; HMM-score: 21.5)
    HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 18)
    MarR; MarR family (PF01047; HMM-score: 14.9)
    Rrf2; Iron-dependent Transcriptional regulator (PF02082; HMM-score: 14.6)
    RPA_C; Replication protein A C terminal (PF08784; HMM-score: 14.1)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14)
    MarR_2; MarR family (PF12802; HMM-score: 12.9)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.9)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 11.6)
    Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by BirA, TF important in Biotin biosynthesis: see SACOL1496

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9944
    • Cytoplasmic Membrane Score: 0.0018
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0032
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010951
    • TAT(Tat/SPI): 0.000494
    • LIPO(Sec/SPII): 0.001853
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1496
  • protein localization: see SACOL1496
  • quantitative data / protein copy number per cell: see SACOL1496
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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