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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1496 [new locus tag: SACOL_RS07620 ]
  • pan locus tag?: SAUPAN003916000
  • symbol: birA
  • pan gene symbol?: birA
  • synonym:
  • product: birA bifunctional protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1496 [new locus tag: SACOL_RS07620 ]
  • symbol: birA
  • product: birA bifunctional protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1537306..1538277
  • length: 972
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCAAAATATAGTCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATA
    TCTGGACAAAGCATTGCGGAATCACTTAATATTTCACGCACTGCAGTAAAAAAAGTGATT
    GACCAATTAAAGTTAGAGGGATGTAAAATAGATTCAGTAAATCATAAAGGGCATTTATTA
    CAACAGCTCCCAGATATTTGGTATCAAGGTATAATAGACCAATATACAAAAAGTTCTGCT
    TTGTTTGATTTTAGTGAAGTATACGATTCAATAGATTCTACACAACTTGCTGCGAAAAAG
    TCACTTGTTGGAAATCAATCTTCATTTTTTATCTTGAGTGATGAACAAACGAAAGGTCGT
    GGGCGATTTAATAGACATTGGAGTTCTTCAAAAGGGCAAGGACTTTGGATGTCTGTCGTG
    TTAAGACCTAACGTTGCATTCTCAATGATATCTAAATTTAATTTATTTATTGCATTAGGG
    ATAAGAGATGCGATTCAACATTTTAGTCAAGATGAAGTCAAAGTGAAATGGCCGAATGAT
    ATATATATTGATAATGGTAAAGTGTGTGGTTTCTTAACTGAAATGGTTGCTAATAATGAT
    GGTATAGAAGCAATAATATGTGGTATAGGTATTAATTTGACGCAACAACTAGAAAACTTT
    GATGAAAGTATTAGACATAGAGCAACAAGTATACAATTACATGATAAAAATAAATTAGAT
    AGATATCAATTTTTAGAGATATTACTTCAAGAAATTGAAAAAAGATATAATCAATTTTTA
    ACGTTACCTTTTTCTGAAATTCGTGAAGAATATACTGCAGCTTCTAATATTTGGAATAGA
    ACGTTGCTATTTACAGAAAATGATAAACAGTTTAAAGGACAAGCAATTGATTTAGATTAC
    GATGGCTATCTAATTGTTAGAGATGAAGCGGGTGAATCACACCGTTTAATTAGTGCAGAT
    ATAGATTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    972

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1496 [new locus tag: SACOL_RS07620 ]
  • symbol: BirA
  • description: birA bifunctional protein
  • length: 323
  • theoretical pI: 5.92816
  • theoretical MW: 37086.7
  • GRAVY: -0.360681

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.15?  ExPASy
    Biotin--[acetyl-CoA-carboxylase] ligase ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair biotin--[acetyl-CoA-carboxylase] ligase (TIGR00121; EC 6.3.4.15; HMM-score: 180.1)
    and 4 more
    Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 32.2)
    Signal transduction Regulatory functions DNA interactions TyrR family helix-turn-helix domain (TIGR04381; HMM-score: 15.1)
    Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 12.5)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 11.7)
  • TheSEED  :
    • Biotin operon repressor
    • Biotin--protein ligase (EC 6.3.4.9)(EC 6.3.4.10)(EC 6.3.4.11)(EC 6.3.4.15)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Biotin operon repressor
    and 2 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Biotin--protein ligase (EC 6.3.4.9)(EC 6.3.4.10)(EC 6.3.4.11)(EC 6.3.4.15)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Biotin--protein ligase (EC 6.3.4.9, EC 6.3.4.10, EC 6.3.4.11, EC 6.3.4.15)
  • PFAM:
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 53.4)
    tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 48.7)
    and 13 more
    BPL_LplA_LipB_2; Biotin/lipoate A/B protein ligase family (PF16917; HMM-score: 21.8)
    TRB (CL0206) BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 19.7)
    HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.3)
    Rrf2; Transcriptional regulator (PF02082; HMM-score: 17.1)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 15.8)
    MarR; MarR family (PF01047; HMM-score: 14.8)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.7)
    RPA_C; Replication protein A C terminal (PF08784; HMM-score: 13.6)
    Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 13.2)
    no clan defined PulG; Type II secretory pathway pseudopilin (PF11773; HMM-score: 12.8)
    HTH (CL0123) HTH_46; Winged helix-turn-helix DNA binding (PF15977; HMM-score: 12.3)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010951
    • TAT(Tat/SPI): 0.000494
    • LIPO(Sec/SPII): 0.001853
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [2] [3] [4]
  • quantitative data / protein copy number per cell: 39 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jingheng Wang, Dorothy Beckett
    A conserved regulatory mechanism in bifunctional biotin protein ligases.
    Protein Sci: 2017, 26(8);1564-1573
    [PubMed:28466579] [WorldCat.org] [DOI] (I p)
  2. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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