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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1497 [new locus tag: SACOL_RS07625 ]
  • pan locus tag?: SAUPAN003917000
  • symbol: papS
  • pan gene symbol?: papS
  • synonym:
  • product: tRNA CCA-pyrophosphorylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1497 [new locus tag: SACOL_RS07625 ]
  • symbol: papS
  • product: tRNA CCA-pyrophosphorylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1538264..1539466
  • length: 1203
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGT
    TTTGAAGCATATTATGTAGGTGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCAT
    GATATAGATATCACAACAAGTGCAACGCCGGATGAAATAGAATCTATCTTTAGTCATACG
    ATACCTGTAGGTAAAGAACATGGCACGATAAATGTAGTTTTTAATGATGAAAATTATGAA
    GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACA
    TTTGTTCGTGATTTATACGAAGATTTGCAACGACGAGATTTCACGATGAATGCGATAGCA
    ATGGATACAGCATACAAATTGTATGATTATTTTGATGGTCAACAAGATATTAATAATCGA
    ATAATAAGAACTGTAGGTATAGCTGAGGAACGATTCCAAGAAGATGCTTTACGTATGATT
    CGATGTTTAAGGTTCCAGTCACAATTATCATTTGATATTGCAACGGAAACATTCGAAGCG
    ATGCGTATACAAATGGCAGATATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTA
    ACTAAATTAATGCGAGGTATTAATGTTGAAAAGAGTTTTAATCATTTAAAATCGCTGAAA
    GCATTTAATTATATGCCGTATTTCGAACATCTTGATATGAATCAAATTAATGTAACTGAA
    GCAATTGATTTAGAATTGTTGATTGCTATAGTATCAGTTAAATTTGATATTAATTACTCA
    TTGAAGCCTTTAAAGCTAAGTAACCGACAAGTAAAAGATATCAATCAATATATTCAAATT
    ATGAATGCATTACCAAGTATTATTACAAAAGAACAATTAAAAATGTTTGTTTATGATTAT
    GATACGCATCTCATTAAAAATGTAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
    CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCGTTTA
    CCTATGCATAATAGAAAAGACATGATGGTTAATGGCGGTGTACTTATGGCACATTTAAAT
    GCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAATTGAGATAGCGATTGTAACA
    GGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1497 [new locus tag: SACOL_RS07625 ]
  • symbol: PapS
  • description: tRNA CCA-pyrophosphorylase
  • length: 400
  • theoretical pI: 5.1044
  • theoretical MW: 46435.9
  • GRAVY: -0.247

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.7.72?  ExPASy
    CCA tRNA nucleotidyltransferase A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate
  • TIGRFAM:
    poly(A) polymerase (TIGR01942; EC 2.7.7.19; HMM-score: 161.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification CCA tRNA nucleotidyltransferase (TIGR02692; EC 2.7.7.72; HMM-score: 161.1)
  • TheSEED  :
    • tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)
    RNA Metabolism RNA processing and modification tRNA nucleotidyltransferase  tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)
  • ⊞PFAM:
    NTP_transf (CL0260) PolyA_pol; Poly A polymerase head domain (PF01743; HMM-score: 132.5)
    and 2 more
    HD_PDEase (CL0237) tRNA_NucTran2_2; tRNA nucleotidyltransferase domain 2 putative (PF13735; HMM-score: 84.5)
    no clan defined PolyA_pol_RNAbd; Probable RNA and SrmB- binding site of polymerase A (PF12627; HMM-score: 37)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9607
    • Cytoplasmic Membrane Score: 0.0335
    • Cell wall & surface Score: 0.0013
    • Extracellular Score: 0.0045
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004999
    • TAT(Tat/SPI): 0.000857
    • LIPO(Sec/SPII): 0.000527
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • ⊟interaction partners:
    SACOL2222(rpsE)30S ribosomal protein S5  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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