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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1199 [new locus tag: SA_RS06820 ]
  • pan locus tag?: SAUPAN003762000
  • symbol: SA1199
  • pan gene symbol?: trpE
  • synonym:
  • product: anthranilate synthase component I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1199 [new locus tag: SA_RS06820 ]
  • symbol: SA1199
  • product: anthranilate synthase component I
  • replicon: chromosome
  • strand: +
  • coordinates: 1372565..1373971
  • length: 1407
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGATATATTTTATAAAAAAATAAAAGCAAATGTAACGCCCGAAGTTTTAGCACAACTT
    CATTCCAAGAAGATCATTTTGGAAAGTACAAATCAACAACAAACTAAAGGTCGCTATTCA
    GTTGTTATTTTTGATATTTATGGCACTTTAACTTTAGATAATGATGTATTATCAGTAAGC
    ACTTTAAAAGAATCGTATCAAATCATTGAAAGACCGTACCATTATTTAACGACTAAAATA
    AATGAAGACTACCATAATATTCAAGATGAGCAACTTAAGTCATTACCGTTTATATCTGGA
    TATGTTGGGACGTGTAGCTTTGATTTAGTAAGACATGAATTTCCTAAATTGCAATCAATA
    CAATTAGAAGATCACAAGCAGCACGATGTAAGGTTATATATGGTTGAACAAGTTTATGTA
    TTTGACCATTACAAAGATGAGTTATATATCATCGCGACGAATCAATTTTCAAATTCAACA
    AAATCAGATCTTGAGAATCGAGTTAATAAGTCTATCGAAGACTTAACTAAAATCCAACCA
    TTCATGCCTACACAAGATTTTGATTTTAAAACTAAAGAAATTCAATCAAATATTTCTGAA
    GAAAGATTTATCGAAATGATTCAGTATTTCAAAGAGAAAATAACAGAAGGGGATATGTTC
    CAAGTTGTGCCATCAAGAATTTACAAATATGCGCATCATGCTAGTCAGCATTTAAATCAA
    CTTTCGTTTCAACTGTATCAAAATTTAAAACGACAAAACCCAAGTCCATATATGTATTAT
    CTTAATATCGATCAACCATATATTGTCGGTAGTTCTCCCGAAAGTTTCGTAAGTGTCAAA
    GATCAAATTGTAACAACTAATCCTATTGCAGGTACGATTCAACGTGGTGAGACGACACAA
    ATAGATAATGAGAATATGAAACAACTACTTAATGATCCAAAAGAATGCAGCGAACATCGT
    ATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAA
    ATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGAAATC
    ACAGGTAAAATAAATCAAAATTTATCACCAATGACAGTTATTGCGAATTTATTACCAACA
    GGTACCGTTTCAGGTGCACCAAAATTACGTGCAATTGAAAGAATATATGAACAATATCCA
    CATAAACGAGGCGTTTATAGTGGTGGTGTTGGATACATAAATTGTAATCATAACTTAGAT
    TTTGCTTTAGCAATTCGAACGATGATGATAGATGAGCAGTATATCAACGTAGAAGCTGGT
    TGTGGCGTTGTATATGATTCTATTCCTGAAAAAGAACTGAATGAAACGAAATTGAAAGCT
    AAAAGCTTATTGGAGGTGAGGCCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1407

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1199 [new locus tag: SA_RS06820 ]
  • symbol: SA1199
  • description: anthranilate synthase component I
  • length: 468
  • theoretical pI: 6.61777
  • theoretical MW: 54206.4
  • GRAVY: -0.465598

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 442)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 375.6)
    and 7 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 270.2)
    aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 260.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 241.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 192.3)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 182.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone isochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 181)
    salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 148.6)
  • TheSEED  :
    • Anthranilate synthase, aminase component (EC 4.1.3.27)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Tryptophan synthesis  Anthranilate synthase, aminase component (EC 4.1.3.27)
  • ⊞PFAM:
    no clan defined Chorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 290)
    and 2 more
    Anth_synt_I_N; Anthranilate synthase component I, N terminal region (PF04715; HMM-score: 100.5)
    HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.92
    • Cytoplasmic Membrane Score: 0.0579
    • Cell wall & surface Score: 0.0023
    • Extracellular Score: 0.0197
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002526
    • TAT(Tat/SPI): 0.000135
    • LIPO(Sec/SPII): 0.000459
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIFDIYGTLTLDNDVLSVSTLKESYQIIERPYHYLTTKINEDYHNIQDEQLKSLPFISGYVGTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNSTKSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGDMFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESFVSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVSKIGTSKITKLMVIEKYEHVMHIVSEITGKINQNLSPMTVIANLLPTGTVSGAPKLRAIERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPEKELNETKLKAKSLLEVRP

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulators: T-box(Trp) (transcription antitermination) regulon, CodY (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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