PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_2298 [new locus tag: NWMN_RS13215 ]
pan locus tag^?: SAUPAN005930000
symbol: cysG
pan gene symbol^?: nasF
• synonym:
• product: uroporphyrin-III C-methyl transferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2298 [new locus tag: NWMN_RS13215 ]
• symbol: cysG
• product: uroporphyrin-III C-methyl transferase
• replicon: chromosome
• strand: -
• coordinates: 2525722..2526699
• length: 978
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332196 NCBI
• RefSeq: YP_001333332 NCBI
• BioCyc:
• MicrobesOnline: 3707900 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTCTGTAGAGGAATATGGCAAGGTTTACTTGATAGGTGCGGGTCCAGGTAATCCGAAT
  TATTTGACGAAAAAGGCTGAGCGTTTAATACGTGAAGCGGATGTCATTCTATATGATCGA
  TTAGTGAATCCGTTAATTTTACAATATGCGAATTTGACTACTGAAATTATCGATGTTGGT
  AAAAAGCCGTATGCAAAACATATTCAGCAAGAAAAAATAAATGACTGTATCGTGGAAGCA
  GCGCGTCGATATAACAAGGTTGTTAGACTTAAAGGTGGCGATCCAGCGATATTTGGTCGT
  GTGCAAGAAGAAGTCGACACACTAAATGAACATCATATTGCGTTTGAAATTGTACCAGGG
  GTGACATCAGCGAGCGCAGCAGTTGCTACTATGCAGACAGGTTTGACAATGCGTACAGTT
  GCTAAAAGTGTGACATTTTCTACAGGTCACTTTAAAGATTCAGAAGAAAATGAAGTGGAT
  GTCAATTCCTTAGTGAATGGTGGCACGTTAGCAATTTATATGGGGGTAAAACGTTTAGGG
  AAAATTATCACACAGATACAACAATACACAGATATTGATTACCCGATAGCGATTGTCTTT
  CAAGCATCGTGTTTTAACGAGTTTGTTGTTAAGGGGCGTTTAAGTAATATTATTTCAAAA
  TTGCAACACTATTCAATTGAGGCGAAACCAGGTATATGTATTATAGGGGAAGTTGTTGAT
  TATACTGAAAACACTCCTAAATCATATGATCCTATGAAGCAATTTTATGTAGTAAGTGGT
  TCTAAACATGACGCCCTTATGCTCTGTGAACATTTATATGACGAAGGTTATGGCTGTTTG
  CTAAACCCAAATGATACATCGAATGGCACATATCATTCGTCGCAATATGATTACTATGAT
  GCATTTATTAAGCAGCAAGAAAATGTAACATATATTTCAACCGATCGTGCAGATGCTAAT
  ACAGTGTTATGTCACTAA
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⊟Protein

⊟General

• locus tag: NWMN_2298 [new locus tag: NWMN_RS13215 ]
• symbol: CysG
• description: uroporphyrin-III C-methyl transferase
• length: 325
• theoretical pI: 5.6022
• theoretical MW: 36335
• GRAVY: -0.237538

⊟Function

• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen-III C-methyltransferase (TIGR01469; EC 2.1.1.107; HMM-score: 297.6)
  and 6 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-4 C11-methyltransferase (TIGR01465; EC 2.1.1.133; HMM-score: 157.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B C17-methyltransferase (TIGR01466; EC 2.1.1.131; HMM-score: 47.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-2 C(20)-methyltransferase (TIGR01467; EC 2.1.1.130; HMM-score: 45)
  precorrin-6A synthase (deacetylating) (TIGR02434; EC 2.1.1.152; HMM-score: 40.6)
  precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit (TIGR02467; EC 2.1.1.132; HMM-score: 39.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03365; HMM-score: 12.7)
• TheSEED  :
  • Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)
• ⊞PFAM:
  no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 176.6)
  and 2 more
  IKBKB_SDD; IQBAL scaffold dimerization domain (PF18397; HMM-score: 16.3)
  DUF6200; Family of unknown function (DUF6200) (PF19702; HMM-score: 15.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9792
  • Cytoplasmic Membrane Score: 0.0097
  • Cell wall & surface Score: 0.001
  • Extracellular Score: 0.0101
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002926
  • TAT(Tat/SPI): 0.000133
  • LIPO(Sec/SPII): 0.001011
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222510 NCBI
• RefSeq: YP_001333332 NCBI
• UniProt: A0A0H3KAA0 UniProt

⊟Protein sequence

• MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQEEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVDVNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cysG < nirD < nirB < nirR

⊟Regulation

• ⊞regulators: Rex* (repression) regulon, NreC* (activation) regulon

  Rex*	(TF)	important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise
  NreC*	(TF)	important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications