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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2015 [new locus tag: NWMN_RS11650 ]
  • pan locus tag?: SAUPAN005402000
  • symbol: mnaA
  • pan gene symbol?: mnaA
  • synonym:
  • product: UDP-GlcNAc 2-epimerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2015 [new locus tag: NWMN_RS11650 ]
  • symbol: mnaA
  • product: UDP-GlcNAc 2-epimerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2232165..2233295
  • length: 1131
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGATGAAAAAGATTATGACCATATTTGGAACAAGACCCGAAGCCATAAAGATGGCTCCT
    TTAGTTAAAGCTTTGGAACAAGAAAAAATGCTCGAGCCAATTGTTGTTGTTACAGCACAG
    CATAGAGAAATGCTTGATTCAGTATTAAGTACCTTTGAAATCAAACCAAAATACGATTTG
    AATATTATGAAATCAGGACAGACACTTTCTGAGATAACTTCTAAATCAATCACTCAATTA
    GAACAAGTGATTCAATTAGAAAAACCAGACATGGTACTTGTACATGGTGATACGATGACG
    ACATTTGCAGGTGGTTTAGCGGCATTTTATAACCAAGTGCCGATTGGACACGTTGAAGCA
    GGGCTTAGAAGTTATGATAAATATTCACCTTTTCCAGAAGAGGTGAATAGACAATTAGTT
    GGTGTATTAGCTGACTTACATTTTGCACCTACTAAAAATGCTGCATCGCATTTGTTAAGT
    GAAGGGAAATATTCAGAAAGTGTTGTTGTCACTGGTAATACTGCGATTGATGCTATGAAA
    TATACAGTTGATGACAATTATAAATCTAACATCATGGATAAATACCATGACAAAAAATTC
    ATTCTTATGACAGCACACCGACGAGAAAATATCGGGAAACCCATGGAAAATATATTTAAA
    GCGGTTAGACGCTTAATAGATGAGTATACTGATTTAGCACTGGTATATCCAATGCATAAA
    AACCCGAAAGTTCGAGAGGTGGCTCAAAAGATTTTAGGTAGTCATGACAGAATTGAATTG
    ATTGAACCTTTGGACGTAGTAGATTTCCATAATTTTGCTAAAAAATCTTATTTTATTTTG
    ACAGATTCAGGTGGAATTCAGGAAGAAGCGCCATCATTTAATAAGCCAGTTTTAGTTTTG
    CGAAGTGTTACAGAACGTCCTGAAGGTGTTGAAGCTGGCACATTAAAAGTCATTGGAACA
    AATAAGCAAAATGTGTATCAAGCTGCAAAAGAATTAATCGATGATGAGAGATTATATCAT
    CAAATGTCTGAAGCATCTAATCCTTACGGTGATGGATTTGCTTCAGAAAGAATAGTTAAT
    CATATTAAGTATTATTTGAATTTAATCACTGAAAAACCAAGCGATTTCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1131

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2015 [new locus tag: NWMN_RS11650 ]
  • symbol: MnaA
  • description: UDP-GlcNAc 2-epimerase
  • length: 376
  • theoretical pI: 6.52172
  • theoretical MW: 42543.6
  • GRAVY: -0.31383

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine 2-epimerase (TIGR00236; EC 5.1.3.14; HMM-score: 529.4)
    and 5 more
    UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing (TIGR03568; EC 3.1.4.-,5.1.3.-; HMM-score: 122.5)
    putative cofactor-binding repeat (TIGR03807; HMM-score: 15.7)
    Metabolism Energy metabolism Amino acids and amines aspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 15)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid-A-disaccharide synthase (TIGR00215; EC 2.4.1.182; HMM-score: 13.9)
    conserved hypothetical protein (TIGR03492; HMM-score: 13.8)
  • TheSEED  :
    • UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)
  • ⊞PFAM:
    GT-B (CL0113) Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 424)
    and 2 more
    no clan defined DUF5420; Family of unknown function (DUF5420) (PF17457; HMM-score: 15.8)
    GT-A (CL0110) NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9912
    • Cytoplasmic Membrane Score: 0.0062
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0024
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007367
    • TAT(Tat/SPI): 0.000222
    • LIPO(Sec/SPII): 0.000479
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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