⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS06605 [old locus tag: SA1163 ]
pan locus tag^?: SAUPAN003698000
symbol: SA_RS06605
pan gene symbol^?: thrD
• synonym:
• product: aspartate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS06605 [old locus tag: SA1163 ]
• symbol: SA_RS06605
• product: aspartate kinase
• replicon: chromosome
• strand: -
• coordinates: 1323919..1325301
• length: 1383
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1323919..1325301) NCBI
• BioCyc: SA_RS06605 BioCyc
• MicrobesOnline: see SA1163

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTT
  TTAAATATTGTTAATTCTGATCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAA
  AGACATGATAATGATATTAAAACAACTGATTTGTTAATCAGATTATATGAAAAGGTCATT
  AATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTATGATGATATTGTA
  AAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTAT
  ATTAATCAATTAAAAAATGAACCAAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAA
  GATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTCAAGGTATACCAACAAAATAT
  ATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAAGCACAAATATTA
  GATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAAAAACTAATTATCCCA
  GGATTTTTTGGCGTATCTCATGAAGGATATATAGTTACATTTCCACGTGGCGGATCAGAT
  ATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCCACAATTTATGAAAATTTCACCGAT
  GTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAATTAATCGAAGAA
  ATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAA
  GCCTTACAACCACTTTATAAAGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCT
  CAAGATAAGGGTACATTTATAGTGCATGATAGAGAAATAAATGCGAAAAATGTGATTAGC
  GGTATAAGTTGTGATAAAGATTTTACTGTCATTAATATCAAAAAGTATTTAATGAATAGA
  CAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGAT
  CATATGCCTTCAGGTATTGATACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAAT
  AAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTGATGTTGATGAGCTAAGTATT
  GAGCATGATTTAGCAATATTAATGATTGTCGGCGAAGGTATGAATAAAGTCATCGGTACT
  GCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAA
  GGTGCTTCGGAAATCTCGATGATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTA
  TTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAAAAAACTTGTGTGATAAAAAC
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1383
  

⊟Protein

⊟General

• locus tag: SA_RS06605 [old locus tag: SA1163 ]
• symbol: SA_RS06605
• description: aspartate kinase
• length: 460
• theoretical pI: 6.38678
• theoretical MW: 52003.3
• GRAVY: -0.28087

⊟Function

• ⊞⊞reaction:
  EC 2.7.2.4?  ExPASy
  Aspartate kinase ATP + L-aspartate = ADP + 4-phospho-L-aspartate
• ⊞⊞TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 285.2)
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 254.9)
  and 2 more
  Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 91.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 20.5)
• TheSEED: see SA1163
• ⊞⊞PFAM:
  no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 140.3)
  and 6 more
  ACT (CL0070) ACT_9; ACT domain (PF22468; HMM-score: 76.2)
  ACT; ACT domain (PF01842; HMM-score: 34.8)
  ACT_7; ACT domain (PF13840; HMM-score: 32.1)
  no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 12.7)
  E-set (CL0159) ScpA_C; C5a peptidase, fibronectin type III domain (PF22143; HMM-score: 12.5)
  no clan defined PI_PP_C; Phosphoinositide phosphatase C-terminal domain (PF18365; HMM-score: 9.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9388
  • Cytoplasmic Membrane Score: 0.0179
  • Cell wall & surface Score: 0.0006
  • Extracellular Score: 0.0426
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003722
  • TAT(Tat/SPI): 0.00028
  • LIPO(Sec/SPII): 0.001227
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446791389 NCBI
• RefSeq: WP_000868645 NCBI
• UniProt: see SA1163

⊟Protein sequence

• MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SA1163

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications