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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1360 [new locus tag: SACOL_RS06925 ]
  • pan locus tag?: SAUPAN003698000
  • symbol: SACOL1360
  • pan gene symbol?: thrD
  • synonym:
  • product: aspartate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1360 [new locus tag: SACOL_RS06925 ]
  • symbol: SACOL1360
  • product: aspartate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1365620..1367002
  • length: 1383
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTT
    TTAAATATTGTTAATTCTGATCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAA
    AGACATGATAATGATATTAAAACAACTGATTTGTTAATCAGATTATATGAAAAGGTCATT
    AATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTATGATGATATTGTA
    AAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTAT
    ATTAATCAATTAAAAAATGAACCAAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAA
    GATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTCAAGGTATACCAACAAAATAT
    ATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAAGCACAAATATTA
    GATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAAAAACTAATTATCCCA
    GGATTTTTTGGCGTATCTCATGAAGGATATATAGTTACATTTCCACGTGGCGGATCAGAT
    ATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCCACAATTTATGAAAATTTCACCGAT
    GTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAATTAATCGAAGAA
    ATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAA
    GCCTTACAACCACTTTATAAAGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCT
    CAAGATAAGGGTACATTTATAGTGCATGATAGAGAAATAAATGCGAAAAATGTGATTAGC
    GGTATAAGTTGTGATAAAGATTTTACCGTTATTAATATCAAAAAGTATTTAATGAATAGA
    CAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGAT
    CATATGCCTTCAGGTATTGATACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAAT
    AAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTGATGTTGATGAGCTAAGTATT
    GAGCATGATTTAGCAATATTAATGATTGTCGGCGAAGGTATGAATAAAGTCATCGGTACT
    GCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAA
    GGTGCTTCGGAAATCTCGATGATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTA
    TTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAAAAAACTTGTGTGATAAAAAC
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1383

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1360 [new locus tag: SACOL_RS06925 ]
  • symbol: SACOL1360
  • description: aspartate kinase
  • length: 460
  • theoretical pI: 6.38678
  • theoretical MW: 52003.3
  • GRAVY: -0.28087

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.4?  ExPASy
    Aspartate kinase ATP + L-aspartate = ADP + 4-phospho-L-aspartate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 285.2)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 254.9)
    and 2 more
    Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 91.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 20.5)
  • TheSEED  :
    • Aspartokinase (EC 2.7.2.4)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine Biosynthesis DAP Pathway  Aspartokinase (EC 2.7.2.4)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Aspartokinase (EC 2.7.2.4)
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 142.4)
    and 2 more
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 36.2)
    ACT_7; ACT domain (PF13840; HMM-score: 31.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003722
    • TAT(Tat/SPI): 0.00028
    • LIPO(Sec/SPII): 0.001227
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 55 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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