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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001310
  • pan locus tag?: SAUPAN003698000
  • symbol: JSNZ_001310
  • pan gene symbol?: thrD
  • synonym:
  • product: aspartate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001310
  • symbol: JSNZ_001310
  • product: aspartate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1317652..1319034
  • length: 1383
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTT
    TTAAATATTGTTAATTCTGATCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAA
    AGACATGATAATGATATTAAAACAACTGATTTGTTAATCAGATTATATGAAAAGGTCATT
    AATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTATGATGATATTGTA
    AAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTAT
    ATTAATCAATTAAAAAATGAACCAAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAA
    GATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTCAAGGTATACCAACAAAATAT
    ATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAAGCACAAATATTA
    GATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAGAAACTAATTATCCCA
    GGATTTTTCGGCGTATCTCATGAAGGATATATAGTTACATTTCCACGTGGCGGATCAGAT
    ATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCTACAATTTATGAAAATTTCACCGAT
    GTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAATTAATCGAAGAA
    ATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAA
    GCCTTACAACCACTTTATAAAGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCT
    CAAGATAAAGGTACATTTATAGTGCATGATAGAGAAATAAATGCGAAAAATGTGATTAGC
    GGTATAAGTTGTGATAAAGATTTTACTGTTATTAATATCAAGAAGTATTTAATGAATAGA
    CAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGAT
    CATATGCCTTCAGGTATTGATACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAAT
    AAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTGATGTTGATGAGCTAAGTATT
    GAGCATGATTTAGCAATTTTAATGATTGTCGGCGAAGGTATGAATAAAGTCATCGGTACT
    GCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAA
    GGTGCTTCGGAAATCTCGATGATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTA
    TTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAAAAAACTTGTGTGATAAAAAC
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1383

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001310
  • symbol: JSNZ_001310
  • description: aspartate kinase
  • length: 460
  • theoretical pI: 6.38678
  • theoretical MW: 52003.3
  • GRAVY: -0.28087

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.4?  ExPASy
    Aspartate kinase ATP + L-aspartate = ADP + 4-phospho-L-aspartate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 285.2)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 254.9)
    and 2 more
    Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 91.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 20.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 140.3)
    and 6 more
    ACT (CL0070) ACT_9; ACT domain (PF22468; HMM-score: 76.2)
    ACT; ACT domain (PF01842; HMM-score: 34.8)
    ACT_7; ACT domain (PF13840; HMM-score: 32.1)
    no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 12.7)
    E-set (CL0159) ScpA_C; C5a peptidase, fibronectin type III domain (PF22143; HMM-score: 12.5)
    no clan defined PI_PP_C; Phosphoinositide phosphatase C-terminal domain (PF18365; HMM-score: 9.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9388
    • Cytoplasmic Membrane Score: 0.0179
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0426
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003722
    • TAT(Tat/SPI): 0.00028
    • LIPO(Sec/SPII): 0.001227
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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