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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus N315

locus tag: SA1025 [new locus tag: SA_RS05840 ]

pan locus tag^?: SAUPAN003452000

symbol: mraY

pan gene symbol^?: mraY

synonym:
product: phospho-N-acetylmuramoyl-pentapeptide- transferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SA1025 [new locus tag: SA_RS05840 ]
symbol: mraY
product: phospho-N-acetylmuramoyl-pentapeptide- transferase
replicon: chromosome
strand: +
coordinates: 1160580..1161545
length: 966
essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

Gene ID: 1123856 NCBI
RefSeq: NP_374298 NCBI
BioCyc: see SA_RS05840
MicrobesOnline: 103324 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
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961
ATGATTTTTGTATATGCGTTATTAGCGCTAGTGATTACATTTGTTTTGGTACCTGTTTTA
ATACCTACATTAAAAAGGATGAAATTTGGTCAAAGTATTCGAGAAGAAGGTCCACAAAGC
CATATGAAGAAGACTGGTACACCAACGATGGGTGGACTAACATTTCTATTAAGTATTGTG
ATAACGTCTTTGGTGGCTATTATATTTGTAGATCAAGCTAATCCAATCATACTGTTATTA
TTTGTGACGATTGGTTTTGGGTTAATTGGTTTTATAGATGATTATATTATTGTTGTTAAA
AAGAATAACCAAGGTTTAACAAGTAAACAGAAGTTTTTGGCGCAAATTGGTATTGCGATT
ATTTTCTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATT
CCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGG
CAAGTAGGTTTTTCTAATGCGGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGA
CTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCA
ATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATT
AACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCT
ACGATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTA
ATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATA
TTTAAAATGAGTCCGATTCATCATCATTTTGAATTGATAGGATGGAGCGAATGGAAAGTA
GTTACAGTATTTTGGGCTGTTGGTCTGATTTCAGGTTTAATCGGTTTATGGATTGGAGTG
CATTAA
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966

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SA1025 [new locus tag: SA_RS05840 ]
symbol: MraY
description: phospho-N-acetylmuramoyl-pentapeptide- transferase
length: 321
theoretical pI: 9.37663
theoretical MW: 35232.2
GRAVY: 1.09564

⊟Function[edit | edit source]

⊞reaction:
EC 2.7.8.13? ExPASy
Phospho-N-acetylmuramoyl-pentapeptide-transferase UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
⊞TIGRFAM:
Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan phospho-N-acetylmuramoyl-pentapeptide-transferase (TIGR00445; EC 2.7.8.13; HMM-score: 340.8)
and 1 more
Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase (TIGR02380; EC 2.7.8.33; HMM-score: 46)
TheSEED :
- Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)
Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)
⊞PFAM:
no clan defined Glycos_transf_4; Glycosyl transferase family 4 (PF00953; HMM-score: 138.9)
and 2 more
MraY_sig1; Phospho-N-acetylmuramoyl-pentapeptide-transferase signature 1 (PF10555; HMM-score: 14.8)
DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 10
⊞
DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.9999
- Cell wall & surface Score: 0
- Extracellular Score: 0.0001
⊞
LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.030264
- TAT(Tat/SPI): 0.000548
- LIPO(Sec/SPII): 0.027617
predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

GI: 15926765 NCBI
RefSeq: NP_374298 NCBI
UniProt: P68783 UniProt

⊟Protein sequence[edit | edit source]

MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKKTGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKNNQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFWQVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPYNINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLTGKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH

⊟Experimental data[edit | edit source]

experimentally validated: data available for NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: mraY > murD > div1b > ftsA > ftsZ

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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