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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
- pan locus tag?: SAUPAN005402000
- symbol: SACOL2103
- pan gene symbol?: mnaA
- synonym:
- product: UDP-GlcNAc 2-epimerase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
- symbol: SACOL2103
- product: UDP-GlcNAc 2-epimerase
- replicon: chromosome
- strand: -
- coordinates: 2163782..2164909
- length: 1128
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237484 NCBI
- RefSeq: YP_186918 NCBI
- BioCyc: see SACOL_RS11005
- MicrobesOnline: 913579 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1081ATGAAAAAGATTATGACCATATTTGGAACAAGACCCGAAGCCATAAAGATGGCTCCTTTA
GTTAAAGCTTTGGAACAAGAAAAAATGCTCGAGCCAATTGTTGTTGTTACAGCACAGCAT
AGAGAAATGCTTGATTCAGTATTAAGTACCTTTGAAATCAAACCAAAATACGATTTGAAT
ATTATGAAATCAGGACAGACACTTTCTGAGATAACTTCTAAATCAATCACTCAATTAGAA
CAAGTGATTCAATTAGAAAAACCAGACATGGTACTTGTACATGGTGATACGATGACGACA
TTTGCAGGTGGTTTAGCGGCATTTTATAACCAAGTGCCGATTGGACACGTTGAAGCAGGG
CTTAGAAGTTATGATAAATATTCACCTTTTCCAGAAGAGGTGAATAGACAATTAGTTGGT
GTATTAGCTGACTTACATTTTGCACCTACTAAAAATGCTGCATCGCATTTGTTAAGTGAA
GGGAAATATTCAGAAAGTGTTGTTGTCACTGGTAATACTGCGATTGATGCTATGAAATAT
ACAGTTGATGACAATTATAAATCTAACATCATGGATAAATACCATGACAAAAAATTCATT
CTTATGACAGCACACCGACGAGAAAATATCGGGAAACCCATGGAAAATATATTTAAAGCG
GTTAGACGCTTAATAGATGAGTATACTGATTTAGCACTGGTATATCCAATGCATAAAAAC
CCGAAAGTTCGAGAGGTGGCTCAAAAGATTTTAGGTAGTCATGACAGAATTGAATTGATT
GAACCTTTGGACGTAGTAGATTTCCATAATTTTGCTAAAAAATCTTATTTTATTTTGACA
GATTCAGGTGGAATTCAGGAAGAAGCGCCATCATTTAATAAGCCAGTTTTAGTTTTGCGA
AGTGTTACAGAACGTCCTGAAGGTGTTGAAGCTGGCACATTAAAAGTCATTGGAACAAAT
AAGCAAAATGTGTATCAAGCTGCAAAAGAATTAATCGATGATGAGAGATTATATCATCAA
ATGTCTGAAGCATCTAATCCTTACGGTGATGGATTTGCTTCAGAAAGAATAGTTAATCAT
ATTAAGTATTATTTGAATTTAATCACTGAAAAACCAAGCGATTTCTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
- symbol: SACOL2103
- description: UDP-GlcNAc 2-epimerase
- length: 375
- theoretical pI: 6.52172
- theoretical MW: 42412.4
- GRAVY: -0.319733
⊟Function[edit | edit source]
- ⊞reaction: EC 5.1.3.14? ExPASy
- ⊞TIGRFAM: Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine 2-epimerase (TIGR00236; EC 5.1.3.14; HMM-score: 529.4)and 5 more
- TheSEED :
- UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)
- ⊞PFAM: GT-B (CL0113) Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 424)and 2 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell: 90 [4]
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SACOL2103 < upp < glyA < SACOL2106
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e)