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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1363 [new locus tag: SACOL_RS06935 ]
  • pan locus tag?: SAUPAN003701000
  • symbol: thrC
  • pan gene symbol?: thrC
  • synonym:
  • product: threonine synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1363 [new locus tag: SACOL_RS06935 ]
  • symbol: thrC
  • product: threonine synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1368479..1369540
  • length: 1062
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGAAGATGGCAAGGATTAGTAGAAGAGTTTAAAGCACATTTACCAGTAAATGAAAAT
    ACACCAAAATTAACATTGAACGAGGGAAATACACCACTCATTCATTGTGAAAATATGTCT
    AAAATACTAGGCATAGATTTATATGTGAAGTATGAAGGTGCCAATCCGACAGGTTCATTT
    AAAGATCGCGGTATGGTAATGGCTGTGACAAAAGCAAAAGAGCAAGGTAAGAAAATTGTA
    ATATGCGCTTCGACTGGAAATACATCAGCGTCTGCAGCAGCATATGCAGCGAGAGCAGGT
    TTAAAAGCTATCGTCGTAATACCAGAAGGTAAAATTGCATTAGGTAAATTGTCGCAAGCA
    GTAATGTATGGTGCAGAAATCGTTTCTATTGAAGGAAACTTTGATGAAGCTTTAGAAATT
    GTAAAAGAAATTGCAAAAAGTGGCGAAATCGAGCTTGTAAACTCTGTCAATCCATTTAGA
    ATCGAAGGACAAAAGACAGGCTCATTTGAAATTGTACAACAATTAGACGGTGAAGCACCT
    GATATTTTAGCGATTCCTGTAGGTAATGCAGGTAATATTACTGCATATTGGAAAGGCTTT
    AAAGAATATCATGAAGCTAAAGGATCACAATTGCCGAAAATGTTTGGCTTCCAAGCTGAA
    GGCGCATCACCAATTGTTCAAAATAAAGTCATTAAAAATCCTGAAACGATTGCAACTGCT
    ATTCGAATTGGTAATCCTGCTAGTTGGGATAAGGCGACTAATGCTCTTAAAGAATCAAAT
    GGATTAATAGATAGTGTTACTGATGATGAAATTCTAGAAGCATATCAGTTAATGACAACT
    AAAGAAGGTGTCTTTAGTGAACCAGCGAGTAATGCTTCTATTGCAGGTTTAATTAAATTG
    CATAGACAAGGTAAATTACCTCAAGGTAAAAAAGTAGTTGCTATTTTAACTGGTAATGGA
    TTAAAAGATCCTGATACTGCTATTTCACTACTAGATAATCCGATAAAGCCATTGCCAAAT
    GATAAAGATAGCATTATCGATTATATTAAAGGAGCTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1062

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1363 [new locus tag: SACOL_RS06935 ]
  • symbol: ThrC
  • description: threonine synthase
  • length: 353
  • theoretical pI: 6.1777
  • theoretical MW: 37869.2
  • GRAVY: -0.172805

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 4.2.3.1?  ExPASy
    Threonine synthase O-phospho-L-homoserine + H2O = L-threonine + phosphate
  • ⊞TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 352.3)
    and 17 more
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 105.6)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 85.8)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 78.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 78.7)
    Metabolism Energy metabolism Methanogenesis cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 78.7)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 77)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 72.7)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 69.4)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 60.3)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 54.2)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 52.3)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 48.5)
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 36.8)
    Metabolism Central intermediary metabolism Other 1-aminocyclopropane-1-carboxylate deaminase (TIGR01274; EC 3.5.99.7; HMM-score: 29.8)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 26.1)
    Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 23)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 20.1)
  • TheSEED  :
    • Threonine synthase (EC 4.2.3.1)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Threonine synthase (EC 4.2.3.1)
  • ⊞PFAM:
    NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 267.9)
    and 4 more
    Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 13.4)
    no clan defined PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 13.2)
    DUF4687; Domain of unknown function (DUF4687) (PF15747; HMM-score: 12.6)
    Leu-IlvD (CL0364) PA; PA domain (PF02225; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9766
    • Cytoplasmic Membrane Score: 0.0061
    • Cell wall & surface Score: 0.0007
    • Extracellular Score: 0.0166
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008553
    • TAT(Tat/SPI): 0.000483
    • LIPO(Sec/SPII): 0.000737
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILGIDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLKAIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFRIEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQAEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQLMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNPIKPLPNDKDSIIDYIKGAL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 2435 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 28.69 h [5]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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