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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman

locus tag: NWMN_RS08385 [old locus tag: NWMN_1489 ]

pan locus tag^?: SAUPAN004173000

symbol: NWMN_RS08385

pan gene symbol^?: holA

synonym:
product: DNA polymerase III subunit delta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_RS08385 [old locus tag: NWMN_1489 ]
symbol: NWMN_RS08385
product: DNA polymerase III subunit delta
replicon: chromosome
strand: -
coordinates: 1657201..1658175
length: 975
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_009641 (1657201..1658175) NCBI
BioCyc:
MicrobesOnline: see NWMN_1489

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGAGCGACAATATTGTAGCTATTTATGGAGATGTGCCTGAATTGGTTGAAAAACAAAGT
GCAGAAATCATATCACAATTTTTGAAAAGTGATAGAGATGACTTTAACTTTGTGAAATAT
AATTTATACGAAACAGAGATTGCACCAATTGTTGAAGAAACATTAACATTGCCTTTCTTT
TCAGATAAAAAAGCAATTTTGGTTAAAAATGCATATATATTTACAGGTGAAAAAGCGCCA
AAAGATATGGCTCATAATGTAGATCAATTAATAGAATTTATTGAAAAATATGATGGCGAA
AATTTGATTGTCTTTGAGATATATCAAAATAAACTTGATGAAAGAAAAAAGTTAACTAAA
ACTCTAAAAAAGCATGCAAGGCTTAAAAAAATAGAGCAAATGTCTGAAGAAGAAATAAAA
AAATGGATTCAAAGTAAATTAAATGAGAATTTCAAAGATATCAAAAGAGATGCATTAGAT
TTATTTATTGAGTTGACAGGTATTAACTTTAATATTGTCTCACAAGAGATAGAAAAGTTG
ATTTTATTTTTAGGCGATAGACCAACAATTAATAAGCAGGATGTTAACCAAATTATTAAT
AGAAGTTTAGAGCAAAATGTATTTTTACTGACTGAATACATTCAGAAAAGAAAGAAAGAA
CAAGCAATTCATTTAGTAAAAGATTTAATAACTATGAAAGAAGAACCAATTAAATTACTT
GCACTAATTACAAGTAATTACCGATTATTTTATCAATGTAAGATTCTGAGTCAAAAAGGA
TATAGTGGACAGCAAATTGCTAAAACAATAGGAGTACATCCATACAGAGTAAAATTAGCG
TTAGGACAAGTAAGACATTATCAACTTGATGAATTATTGAATATTATAGATGCTTGTGCG
GAAACTGATTATAAACTTAAATCATCATATATGGATAAACAGTTAATACTGGAATTATTC
ATTCTATCTTTATAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_RS08385 [old locus tag: NWMN_1489 ]
symbol: NWMN_RS08385
description: DNA polymerase III subunit delta
length: 324
theoretical pI: 6.69588
theoretical MW: 37935.8
GRAVY: -0.300309

⊟Function[edit | edit source]

⊞TIGRFAM:
Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta subunit (TIGR01128; EC 2.7.7.7; HMM-score: 300.7)
and 1 more
Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 15.2)
TheSEED: see NWMN_1489
⊞PFAM:
post-AAA (CL0604) DNA_pol3_delta_C; DNA polymerase III delta subunit, C-terminal domain (PF21694; HMM-score: 149.7)
P-loop_NTPase (CL0023) DNA_pol3_delta; DNA polymerase III, delta subunit (PF06144; HMM-score: 120.4)
and 9 more
MmeI_HD-like (CL0884) YtxK_like; YtxK N-terminal helical domain (PF21106; HMM-score: 17.9)
no clan defined DUF2018; Domain of unknown function (DUF2018) (PF09442; HMM-score: 15.5)
DisA-linker; DisA bacterial checkpoint controller linker region (PF10635; HMM-score: 13.7)
KNTase_C (CL0291) YgxA-like_sub_bind; YgxA-like substrate binding domain (PF22339; HMM-score: 13.3)
no clan defined DUF3781; Protein of unknown function (DUF3781) (PF12636; HMM-score: 12.5)
P-loop_NTPase (CL0023) Sigma54_activ_2; Sigma-54 interaction domain (PF14532; HMM-score: 11.9)
no clan defined DUF7529; Family of unknown function (DUF7529) (PF24373; HMM-score: 11.7)
P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 11.1)
no clan defined DUF3819; CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819 (PF12842; HMM-score: 11.1)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9711
- Cytoplasmic Membrane Score: 0.022
- Cell wall & surface Score: 0.0036
- Extracellular Score: 0.0033
LocateP:
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001604
- TAT(Tat/SPI): 0.000156
- LIPO(Sec/SPII): 0.000284
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 447205306 NCBI
RefSeq: WP_001282562 NCBI
UniProt: see NWMN_1489

⊟Protein sequence[edit | edit source]

MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYETEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENLIVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALDLFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRKKEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYRVKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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