PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_00017
pan locus tag^?: SAUPAN000025000
symbol: rplI
pan gene symbol^?: rplI
• synonym:
• product: 50S ribosomal protein L9

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_00017
• symbol: rplI
• product: 50S ribosomal protein L9
• replicon: chromosome
• strand: +
• coordinates: 20282..20734
• length: 453
• essential: no  DEG other strains

⊟Accession numbers

• Gene ID: 3919188 NCBI
• RefSeq: YP_498623 NCBI
• BioCyc: G1I0R-15 BioCyc
• MicrobesOnline: 1288515 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAGTAATTTTTACACAAGATGTTAAAGGTAAAGGTAAAGGTAAAAAAGGTGAAGTT
  AAAGAAGTACCAGTAGGTTATGCAAATAACTTCTTATTGAAAAAGAATTATGCTGTAGAA
  GCAACACCAGGTAACCTTAAACAATTAGAGTTACAGAAAAAACGTGCAAAACAAGAACGC
  CAACAAGAAATTGAAGATGCTAAAGCATTAAAAGAAACGTTATCAAACATTGAAGTTGAA
  GTATCAGCAAAAACTGGTGAAGGTGGTAAATTGTTTGGGTCAGTAAGTACAAAACAAATT
  GCCGAAGCACTAAAAGCACAACATGATATTAAAATTGATAAACGTAAAATGGATTTACCA
  AATGGAATTCATTCCCTAGGATATACGAATGTACCTGTTAAATTAGATAAAGAAGTTGAA
  GGTACAATTCGCGTACACACAGTTGAACAATAA
  60
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  453
  

⊟Protein

⊟General

• locus tag: SAOUHSC_00017
• symbol: RplI
• description: 50S ribosomal protein L9
• length: 150
• theoretical pI: 10.1533
• theoretical MW: 16639.1
• GRAVY: -0.701333

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bL9 (TIGR00158; HMM-score: 144)
• TheSEED  :
  • LSU ribosomal protein L9p
  Protein Metabolism Protein biosynthesis Ribosome LSU bacterial  LSU ribosomal protein L9p
• ⊞PFAM:
  no clan defined Ribosomal_L9_C; Ribosomal protein L9, C-terminal domain (PF03948; HMM-score: 99.8)
  and 1 more
  L9_N_sp (CL0885) Ribosomal_L9_N; Ribosomal protein L9, N-terminal domain (PF01281; HMM-score: 69.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9816
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0179
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008698
  • TAT(Tat/SPI): 0.000399
  • LIPO(Sec/SPII): 0.002571
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 88193838 NCBI
• RefSeq: YP_498623 NCBI
• UniProt: Q2G2T3 UniProt
• STRING: 93061.SAOUHSC_00017 STRING

⊟Protein sequence

• MKVIFTQDVKGKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNLKQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEALKAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• ⊟interaction partners:

  SAOUHSC_02511	(rplD)	50S ribosomal protein L4   (data from MRSA252)
  SAOUHSC_00520	(rplJ)	50S ribosomal protein L10   (data from MRSA252)
  SAOUHSC_00518	(rplK)	50S ribosomal protein L11   (data from MRSA252)
  SAOUHSC_00426		ABC transporter substrate-binding protein   (data from MRSA252)
  SAOUHSC_00679		hypothetical protein   (data from MRSA252)
  SAOUHSC_01806		pyruvate kinase   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAOUHSC_00014 > SAOUHSC_00015 > rplI > SAOUHSC_00018
  predicted SigA promoter  : S7 > SAOUHSC_00013 > S8 > SAOUHSC_00014 > SAOUHSC_00015 > rplI > SAOUHSC_00018
  predicted SigA promoter  : SAOUHSC_00014 > SAOUHSC_00015 > rplI > SAOUHSC_00018

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications