PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1925 [new locus tag: NWMN_RS11110 ]
pan locus tag^?: SAUPAN005188000
symbol: int
pan gene symbol^?: int3
• synonym:
• product: phage integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1925 [new locus tag: NWMN_RS11110 ]
• symbol: int
• product: phage integrase
• replicon: chromosome
• strand: +
• coordinates: 2131160..2132221
• length: 1062
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331189 NCBI
• RefSeq: YP_001332959 NCBI
• BioCyc:
• MicrobesOnline: 3707513 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  TTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAA
  TATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAA
  GCTAATTCTGCTGGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAAC
  TATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCTGTTGTT
  AAAGAAAATACCTACCGTCATTATAGAAATGCATTACAACATATACAAAAACATAAAATA
  GGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTAT
  TCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTA
  GATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAA
  GCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAATATGAAATA
  CTAAAAGATCACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGCTATTCATAATGATT
  TGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGATTGAGCATATCAACCAAGTG
  AAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATC
  GCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCTATGAT
  GGTTACATTTTCAAAGAAGGCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTG
  AAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACT
  CATTGTTCTTATTTACTAGCAAAAGGTGTATCTATACATTACATTTCTAAAAGATTAGGT
  CATAAAAATATAGCAATAACTACATCTGTGTATTCTCATTTGTTAGAAGAAAAATTTAAT
  GAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAA
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⊟Protein

⊟General

• locus tag: NWMN_1925 [new locus tag: NWMN_RS11110 ]
• symbol: Int
• description: phage integrase
• length: 353
• theoretical pI: 10.3453
• theoretical MW: 41340.4
• GRAVY: -0.694901

⊟Function

• ⊞TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 70.4)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 44)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 44)
• TheSEED  :
  • Phage integrase
• ⊞PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 77.7)
  and 6 more
  l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 41.7)
  Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 35.1)
  Phage_int_M; Phage integrase central domain (PF22022; HMM-score: 27.4)
  Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 18.3)
  MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 16.1)
  no clan defined DUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 15.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8789
  • Cytoplasmic Membrane Score: 0.0045
  • Cell wall & surface Score: 0.0045
  • Extracellular Score: 0.112
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007371
  • TAT(Tat/SPI): 0.00365
  • LIPO(Sec/SPII): 0.003029
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222137 NCBI
• RefSeq: YP_001332959 NCBI
• UniProt: A0A0H3K9L2 UniProt

⊟Protein sequence

• MPILRRERMKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNELRSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTNTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEGGSIINLHAINNALKSACRVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1924 > int > NWMN_1926

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications