⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS02900 [old locus tag: SA0494 ]
pan locus tag^?: SAUPAN002305000
symbol: SA_RS02900
pan gene symbol^?: nusG
• synonym:
• product: transcription termination/antitermination protein NusG

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS02900 [old locus tag: SA0494 ]
• symbol: SA_RS02900
• product: transcription termination/antitermination protein NusG
• replicon: chromosome
• strand: +
• coordinates: 575388..575936
• length: 549
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (575388..575936) NCBI
• BioCyc: SA_RS02900 BioCyc
• MicrobesOnline: see SA0494

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  ATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAA
  AATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATC
  TTTAGAGTAGTCATACCGGAAGAAGAAGAAACTCAAGTAAAAGATGGCAAAGCTAAAACG
  ACTGTTAAAAAAACATTCCCTGGATATGTTTTAGTGGAATTAATCATGACAGATGAATCA
  TGGTATGTGGTAAGAAATACACCAGGCGTTACTGGTTTTGTAGGTTCTGCAGGTGCAGGG
  TCTAAGCCAAATCCATTGTTACCAGAAGAAGTTCGCTTCATCTTAAAACAAATGGGTCTT
  AAAGAAAAGACTATCGATGTTGAACTCGAAGTTGGCGAGCAAGTTCGTATTAAATCAGGT
  CCATTTGCGAATCAAGTTGGTGAAGTTCAAGAAATTGAAACAGATAAGTTTAAGCTAACA
  GTATTAGTAGATATGTTTGGCCGAGAAACACCAGTAGAAGTTGAATTCGATCAAATAGAA
  AAGCTTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  549
  

⊟Protein

⊟General

• locus tag: SA_RS02900 [old locus tag: SA0494 ]
• symbol: SA_RS02900
• description: transcription termination/antitermination protein NusG
• length: 182
• theoretical pI: 4.78638
• theoretical MW: 20663.5
• GRAVY: -0.423626

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing Transcription Transcription factors transcription termination/antitermination factor NusG (TIGR00922; HMM-score: 203.6)
  and 3 more
  NusG family protein (TIGR01956; HMM-score: 106.4)
  Genetic information processing Transcription Transcription factors transcription elongation factor/antiterminator RfaH (TIGR01955; HMM-score: 61.1)
  transcription elongation factor Spt5 (TIGR00405; HMM-score: 27)
• TheSEED: see SA0494
• ⊞⊞PFAM:
  NusG-like (CL0439) NusG; Transcription termination factor nusG (PF02357; HMM-score: 103.4)
  and 5 more
  SH3 (CL0010) KOW; KOW motif (PF00467; HMM-score: 30.5)
  SH3_KIN17_C; KIN17 C-terminal SH3 domain (PF25092; HMM-score: 14.3)
  no clan defined DUF3912; Protein of unknown function (DUF3912) (PF13051; HMM-score: 14.2)
  SH3 (CL0010) KOW5_SPT5; Transcription elongation factor SPT5, fifth KOW domain (PF23290; HMM-score: 13.7)
  KOW2_Spt5; Transcription elongation factor SPT5, second KOW domain (PF23284; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9853
  • Cytoplasmic Membrane Score: 0.0011
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0136
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005909
  • TAT(Tat/SPI): 0.000544
  • LIPO(Sec/SPII): 0.00119
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447211046 NCBI
• RefSeq: WP_001288302 NCBI
• UniProt: see SA0494

⊟Protein sequence

• MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVVIPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSKPNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLTVLVDMFGRETPVEVEFDQIEKL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications