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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA1505 [new locus tag: SA_RS08475 ]
  • pan locus tag?: SAUPAN004301000
  • symbol: lysP
  • pan gene symbol?: lysP
  • synonym:
  • product: lysine-specific permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1505 [new locus tag: SA_RS08475 ]
  • symbol: lysP
  • product: lysine-specific permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1712104..1713597
  • length: 1494
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 1124350 NCBI
  • RefSeq: NP_374793 NCBI
  • BioCyc: see SA_RS08475
  • MicrobesOnline: 103819 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCAAAAGTTCAAAATGAAAGTAACAATGTTGTCAAAAGGGGACTTAAAGATCGTCAT
    ATTTCTATGATTGCGATTGGGGGTTGTATTGGTACAGGTTTATTTGTAACTTCTGGTGGA
    GCAATTCATGATGCAGGTGCTTTGGGTGCATTAATAGGATACGCAATTATCGGAATAATG
    GTATTTTTCTTAATGACGTCACTTGGTGAAATGGCTACGTATTTACCAGTATCAGGTTCA
    TTTAGTACATATGCTACAAGATTTGTTGATCCATCTTTAGGGTTTGCGCTTGGTTGGAAC
    TATTGGTTTAACTGGGTAGTAACTGTAGCAGCAGATATTACGATTGCAGCACAAGTCATT
    CAATATTGGACACCATTGCAAGGCATACCCGCTTGGGCATGGAGTGCGTTGTTTTTAGTT
    ATAATTTTTAGTCTGAATTCGTTATCAGTTCGCGTCTATGGTGAAAGTGAATACTGGTTG
    GCATTGATAAAAGTGATTACAGTTATTGTTTTCATTGCAATTGGTTTATTAACGATTGTA
    GGAATCATGGGTGGTCATGTTGTAGGATTTGAAATATTTAATAAAGGTGAAGGTCCTATT
    CTTGGTGGCAACTTAGGAGGAAGTTTGTTATCAATTCTAGGTGTATTCTTAATCGCTGGT
    TTCTCATTCCAAGGTACTGAGTTAATTGGTATTACGGCTGGTGAATCAGAAAATCCTGAA
    CGTGCTGTGCCGAAAGCAATTAAACAAGTATTCTGGAGAATTTTATTATTTTACATTTTA
    GCCATTTTTGTTATCGGTATGTTAATTCCTTATGATAGTAGTGCATTAATGGGTGGTAGT
    GATAATGTAGCAACGTCTCCATTCACATTAGTGTTTAAAAATGCTGGATTTGCGTTTGCA
    GCATCATTTATGAATGCAGTCATTTTAACGTCTGTGTTATCAGCAGGTAACTCAGGTATG
    TATGCTTCAACCCGAATGCTATATTCAATGAGTAAAGATAAATTAGCGTTTGAAGCATTT
    GGTAAAACAAATAAAAACGGTGTGCCATATATGTCGTTACTCGTTACAGCAATCATTGTA
    GTGATTATTTTCGTATTACAAAGCCTTACAAAAGGTGCATACGAATATATCGTTGCAGCA
    AGTGGTTTAACTGGTTTTATTGCTTGGGTAGGTATAGCAGTAAGTCATTATCGATTTAGA
    AGAGCATTTGATAAACAAAATTATGACAAATCTAAATTAAAATATACTGCTAAATTATTC
    CCATTCGGACCATTATTTGCTGGCTTTTTATGTATTGTAGTAATTATTGGTCAAGATGTT
    GACTTTATTAAAACAGGAGATTTTAATTTTAGTCGCTTCTTAATTACGTATATGGGAATT
    CCAGTATTTTTAGCATTCTTTATTTATCATAAGCTTCGATATAAAACGAAAAAAATTCCA
    TTAGAACAAGTAGATTTACGTCAAGACGTGTCAATGGATGAAATAAAAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1494

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1505 [new locus tag: SA_RS08475 ]
  • symbol: LysP
  • description: lysine-specific permease
  • length: 497
  • theoretical pI: 9.51176
  • theoretical MW: 54232.5
  • GRAVY: 0.655936

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 468.2)
    and 10 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 308.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 96.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 69.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 55.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 52.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 52.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 51.5)
    histidine-histamine antiporter (TIGR04298; HMM-score: 25.9)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 14.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssC (TIGR03928; HMM-score: 12)
  • TheSEED  :
    • Lysine-specific permease
  • ⊞PFAM:
    APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 473.3)
    and 1 more
    AA_permease_2; Amino acid permease (PF13520; HMM-score: 150.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9991
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0008
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016178
    • TAT(Tat/SPI): 0.001639
    • LIPO(Sec/SPII): 0.129025
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

  • GI: 15927260 NCBI
  • RefSeq: NP_374793 NCBI
  • UniProt: A0A0H3JQD6 UniProt

⊟Protein sequence[edit | edit source]

  • MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHDAGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYWFNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWLALIKVITVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLIAGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSALMGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDKLAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGIAVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNFSRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • ⊞regulator: L-box (transcription termination) regulon
    L-box(RNA)important in Lysine biosynthesis; RegPrecise 

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1505&oldid=95240"
  • This page was last edited on 11 March 2016, at 10:25.
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