PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08475 [old locus tag: SA1505 ]
• pan locus tag^?: SAUPAN004301000
• symbol: SA_RS08475
• pan gene symbol^?: lysP
• synonym:
• product: gamma-aminobutyrate permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08475 [old locus tag: SA1505 ]
• symbol: SA_RS08475
• product: gamma-aminobutyrate permease
• replicon: chromosome
• strand: -
• coordinates: 1712104..1713597
• length: 1494
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1712104..1713597) NCBI
• BioCyc: SA_RS08475 BioCyc
• MicrobesOnline: see SA1505

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGTCAAAAGTTCAAAATGAAAGTAACAATGTTGTCAAAAGGGGACTTAAAGATCGTCAT
  ATTTCTATGATTGCGATTGGGGGTTGTATTGGTACAGGTTTATTTGTAACTTCTGGTGGA
  GCAATTCATGATGCAGGTGCTTTGGGTGCATTAATAGGATACGCAATTATCGGAATAATG
  GTATTTTTCTTAATGACGTCACTTGGTGAAATGGCTACGTATTTACCAGTATCAGGTTCA
  TTTAGTACATATGCTACAAGATTTGTTGATCCATCTTTAGGGTTTGCGCTTGGTTGGAAC
  TATTGGTTTAACTGGGTAGTAACTGTAGCAGCAGATATTACGATTGCAGCACAAGTCATT
  CAATATTGGACACCATTGCAAGGCATACCCGCTTGGGCATGGAGTGCGTTGTTTTTAGTT
  ATAATTTTTAGTCTGAATTCGTTATCAGTTCGCGTCTATGGTGAAAGTGAATACTGGTTG
  GCATTGATAAAAGTGATTACAGTTATTGTTTTCATTGCAATTGGTTTATTAACGATTGTA
  GGAATCATGGGTGGTCATGTTGTAGGATTTGAAATATTTAATAAAGGTGAAGGTCCTATT
  CTTGGTGGCAACTTAGGAGGAAGTTTGTTATCAATTCTAGGTGTATTCTTAATCGCTGGT
  TTCTCATTCCAAGGTACTGAGTTAATTGGTATTACGGCTGGTGAATCAGAAAATCCTGAA
  CGTGCTGTGCCGAAAGCAATTAAACAAGTATTCTGGAGAATTTTATTATTTTACATTTTA
  GCCATTTTTGTTATCGGTATGTTAATTCCTTATGATAGTAGTGCATTAATGGGTGGTAGT
  GATAATGTAGCAACGTCTCCATTCACATTAGTGTTTAAAAATGCTGGATTTGCGTTTGCA
  GCATCATTTATGAATGCAGTCATTTTAACGTCTGTGTTATCAGCAGGTAACTCAGGTATG
  TATGCTTCAACCCGAATGCTATATTCAATGAGTAAAGATAAATTAGCGTTTGAAGCATTT
  GGTAAAACAAATAAAAACGGTGTGCCATATATGTCGTTACTCGTTACAGCAATCATTGTA
  GTGATTATTTTCGTATTACAAAGCCTTACAAAAGGTGCATACGAATATATCGTTGCAGCA
  AGTGGTTTAACTGGTTTTATTGCTTGGGTAGGTATAGCAGTAAGTCATTATCGATTTAGA
  AGAGCATTTGATAAACAAAATTATGACAAATCTAAATTAAAATATACTGCTAAATTATTC
  CCATTCGGACCATTATTTGCTGGCTTTTTATGTATTGTAGTAATTATTGGTCAAGATGTT
  GACTTTATTAAAACAGGAGATTTTAATTTTAGTCGCTTCTTAATTACGTATATGGGAATT
  CCAGTATTTTTAGCATTCTTTATTTATCATAAGCTTCGATATAAAACGAAAAAAATTCCA
  TTAGAACAAGTAGATTTACGTCAAGACGTGTCAATGGATGAAATAAAAGGTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1494
  

⊟Protein

⊟General

• locus tag: SA_RS08475 [old locus tag: SA1505 ]
• symbol: SA_RS08475
• description: gamma-aminobutyrate permease
• length: 497
• theoretical pI: 9.51176
• theoretical MW: 54232.5
• GRAVY: 0.655936

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 468.2)
  and 10 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 308.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 96.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 69.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 55.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 52.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 52.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 51.5)
  histidine-histamine antiporter (TIGR04298; HMM-score: 25.9)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 14.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssC (TIGR03928; HMM-score: 12)
• TheSEED: see SA1505
• PFAM:
  APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 446.5)
  and 1 more
  AA_permease_2; Amino acid permease (PF13520; HMM-score: 159.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016178
  • TAT(Tat/SPI): 0.001639
  • LIPO(Sec/SPII): 0.129025
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 445971290 NCBI
• RefSeq: WP_000049145 NCBI
• UniProt: see SA1505

⊟Protein sequence

• MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHDAGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYWFNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWLALIKVITVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLIAGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSALMGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDKLAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGIAVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNFSRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: L-box see SA1505

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications