PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1628 [new locus tag: SAUSA300_RS08880 ]
• pan locus tag^?: SAUPAN004301000
• symbol: lysP
• pan gene symbol^?: lysP
• synonym:
• product: lysine-specific permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1628 [new locus tag: SAUSA300_RS08880 ]
• symbol: lysP
• product: lysine-specific permease
• replicon: chromosome
• strand: -
• coordinates: 1780634..1782127
• length: 1494
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914553 NCBI
• RefSeq: YP_494323 NCBI
• BioCyc: see SAUSA300_RS08880
• MicrobesOnline: 1293143 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTCAAAAGTTCAAAATGAAAGTAACAATGTTGTCAAAAGGGGACTTAAAGATCGTCAT
  ATTTCTATGATTGCGATTGGGGGTTGTATTGGTACAGGTTTATTTGTAACTTCTGGTGGA
  GCAATTCATGATGCAGGTGCTTTGGGTGCATTAATAGGATACGCAATTATCGGAATAATG
  GTATTTTTCTTAATGACGTCACTTGGCGAAATGGCTACGTATTTGCCAGTATCAGGTTCA
  TTTAGTACATATGCTACAAGATTTGTTGATCCATCTTTAGGGTTTGCGCTTGGTTGGAAC
  TATTGGTTTAACTGGGTAGTGACTGTAGCAGCAGATATTACGATTGCAGCACAAGTCATT
  CAATATTGGACACCATTGCAAGGCATACCCGCTTGGGCATGGAGTGCGTTGTTCTTAGTT
  ATAATTTTTAGTCTGAATTCGTTATCAGTTCGCGTCTATGGTGAAAGTGAATACTGGTTG
  GCATTGATAAAAGTGGTTACAGTTATTGTTTTCATTGCAATTGGTTTATTAACGATTGTC
  GGAATCATGGGTGGTCATGTTGTAGGATTCGAAATATTTAATAAAGGTGAAGGTCCAATT
  CTTGGTGGCAACTTAGGAGGAAGTTTGTTATCAATTCTAGGTGTATTCTTAATCGCTGGT
  TTCTCATTCCAAGGTACTGAGTTAATTGGTATTACGGCTGGTGAATCAGAAAATCCTGAA
  CGTGCTGTGCCGAAAGCAATTAAACAAGTATTCTGGAGAATTTTATTATTTTACATTTTA
  GCCATTTTTGTTATCGGTATGTTAATTCCTTATGATAGTAGTGCATTAATGGGGGGTAGT
  GATAATGTAGCAACGTCTCCATTCACATTAGTGTTTAAAAATGCTGGATTTGCGTTTGCA
  GCATCATTTATGAATGCAGTCATTTTAACGTCTGTGTTATCAGCAGGTAACTCAGGTATG
  TATGCTTCAACTCGAATGCTATATTCAATGAGTAAAGATAAATTAGCGTTTGAAGCATTT
  GGTAAAACAAATAAAAACGGTGTGCCATATATGTCGTTACTCGTTACAGCAATCATTGTA
  GTGATAATTTTCGTATTACAAAGCCTTACAAAAGGTGCATACGAATATATCGTTGCAGCA
  AGTGGTTTAACTGGTTTTATTGCTTGGGTAGGTATAGCAGTAAGTCATTATCGATTTAGA
  AGAGCATTTGATAAACAAAATTATGACAAATCTAAATTAAAATATACTGCTAAATTATTC
  CCATTCGGACCATTATTTGCTGGCTTTTTATGTATTGTAGTAATTATTGGTCAAGATGTT
  GACTTTATTAAAACAGGAGATTTTAATTTTAGTCGCTTCTTAATTACGTATATGGGAATT
  CCAGTATTTTTAGCATTCTTTATTTATCATAAGCTTCGATATAAAACGAAAAAAATTCCA
  TTAGAACAAGTAGATTTACGTCAAGACGTGTCAATGGATGAAATAAAAGGTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1628 [new locus tag: SAUSA300_RS08880 ]
• symbol: LysP
• description: lysine-specific permease
• length: 497
• theoretical pI: 9.51176
• theoretical MW: 54218.4
• GRAVY: 0.655332

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 467.6)
  and 10 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 308.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 97.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 69.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 56.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 53.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 53.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 51.4)
  histidine-histamine antiporter (TIGR04298; HMM-score: 26.1)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 14.3)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssC (TIGR03928; HMM-score: 12)
• TheSEED  :
  • Lysine-specific permease
• PFAM:
  APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 446.1)
  and 1 more
  AA_permease_2; Amino acid permease (PF13520; HMM-score: 159.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016178
  • TAT(Tat/SPI): 0.001639
  • LIPO(Sec/SPII): 0.129025
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87161865 NCBI
• RefSeq: YP_494323 NCBI
• UniProt: A0A0H2XJI8 UniProt

⊟Protein sequence

• MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHDAGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYWFNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWLALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLIAGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSALMGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDKLAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGIAVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNFSRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: L-box (transcription termination) regulon

  L-box

  (RNA)

  important in Lysine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications