⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_RS07975 [old locus tag: NWMN_1413 ]
pan locus tag^?: SAUPAN004041000
symbol: NWMN_RS07975
pan gene symbol^?: —
• synonym:
• product: AraC family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS07975 [old locus tag: NWMN_1413 ]
• symbol: NWMN_RS07975
• product: AraC family transcriptional regulator
• replicon: chromosome
• strand: +
• coordinates: 1582034..1582927
• length: 894
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1582034..1582927) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1413

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTGAATTAATTGGAGTGAAAGTTTTGGACGTTATCAAGCAAATACAACAGGCAATT
  GTTTATATTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTT
  GGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAA
  GCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTACA
  CGACTTGTAGATATCGCTAAAAAATATCACTATGCAAATTCAAATGATTTTGCAAATGAT
  TTTAGTGATTTTCACGGCGTATCACCTATTCAAGCCTCTACTAAAAAAGATGAATTACAA
  ATTCAAGAGCGATTATATATCAAATTATCAACTACTGAGAGAGCACCTTATCCATACAGA
  TTAGAAGAGACAGATGATATTTCATTGGTTGGATATGCACGATTTATAGACACTAAGTAT
  TTGTCACATCCTTTTAATGTTCCGGATTTTTTAGAAGACTTGCTCATTGATGGTAAAATT
  AAAGAGTTACGACGATATAATGACGTTAGTCCATTTGAACTATTTGTTATTAGTTGTCCT
  CTTGAAAATGGTTTAGAAATATTTGTAGGTGTACCAAGTGAACGTTATCCTGCACACTTA
  GAAAGTCGATTTTTACCTGGCAAACATTGTGCGAAATTCAATTTACAAGGTGAAATTGAT
  TATGCAACTAATGAAGCTTGGTACTATATTGAATCAAGTTTGCAGTTAACATTGCCATAT
  GAACGAAATGATTTATATGTTGAAGTGTACCCTCTCGATATTTCATTTAATGACCCATTC
  ACTAAAATTCAGCTTTGGATTCCTGTTAAACAGAGTCCTTATGACGAAGATTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS07975 [old locus tag: NWMN_1413 ]
• symbol: NWMN_RS07975
• description: AraC family transcriptional regulator
• length: 297
• theoretical pI: 4.5112
• theoretical MW: 34373.7
• GRAVY: -0.282828

⊟Function

• TIGRFAM:
  YSIRK-targeted surface antigen transcriptional regulator (TIGR04094; HMM-score: 35.6)
• TheSEED: see NWMN_1413
• ⊞⊞PFAM:
  SHS2 (CL0319) Cass2; Integron-associated effector binding protein (PF14526; HMM-score: 97.7)
  and 2 more
  HTH (CL0123) HTH_18; Helix-turn-helix domain (PF12833; HMM-score: 45.3)
  HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 24.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8573
  • Cytoplasmic Membrane Score: 0.0976
  • Cell wall & surface Score: 0.0012
  • Extracellular Score: 0.044
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002234
  • TAT(Tat/SPI): 0.000602
  • LIPO(Sec/SPII): 0.000206
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446493254 NCBI
• RefSeq: WP_000571108 NCBI
• UniProt: see NWMN_1413

⊟Protein sequence

• MIELIGVKVLDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKMIVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPDFLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPGKHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQLWIPVKQSPYDED

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications