PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1276 [new locus tag: SA_RS07235 ]
pan locus tag^?: SAUPAN003899000
symbol: SA1276
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1276 [new locus tag: SA_RS07235 ]
• symbol: SA1276
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1482017..1482349
• length: 333
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124114 NCBI
• RefSeq: NP_374557 NCBI
• BioCyc: see SA_RS07235
• MicrobesOnline: 103583 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
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  301
  
  ATGGGTAAAAAAATGGGTCTAGGTTTATCTATAGCATTGGTTGTTATTGGTATTGCCGTT
  GTATGTTTAATGATTTTTTCTAGTCAAAAAACGACTTATTTTGGTTATATGAATAGTAAA
  ACAAATGCAGAAAAAGTTGTCAGTGAAAAAGATGGATTAGTCAAACATAATATCAAAGTA
  GAACCATCAAATGATTTTAAACCGAAAAAAGGAGACTTTGTAAAATTAGTTTCTAAAGAT
  GATGGGAAGACATTTTATAAACAAGAGATTGTTAAACATGATGACGTCCCACACGGTTTA
  ATGATGAAAATTCACGACATGCATATGAATTAA
  60
  120
  180
  240
  300
  333
  

⊟Protein

⊟General

• locus tag: SA1276 [new locus tag: SA_RS07235 ]
• symbol: SA1276
• description: hypothetical protein
• length: 110
• theoretical pI: 9.90462
• theoretical MW: 12330.5
• GRAVY: -0.217273

⊟Function

• TIGRFAM:
  type II secretion system protein I (TIGR01707; HMM-score: 13.1)
• TheSEED  :
  • FIG01108065: hypothetical protein
• ⊞PFAM:
  no clan defined DUF4889; Domain of unknown function (DUF4889) (PF16230; HMM-score: 128.3)
  and 2 more
  BssC_TutF; BssC/TutF protein (PF08201; HMM-score: 12.7)
  OB (CL0021) YqiJ_OB; Inner membrane protein YqiJ, OB-fold (PF07290; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helix: 1
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.8759
  • Cell wall & surface Score: 0.001
  • Extracellular Score: 0.123
• ⊞LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 5
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: Signal peptide LIPO(Sec/SPII) length 21 aa
  • SP(Sec/SPI): 0.327738
  • TAT(Tat/SPI): 0.001323
  • LIPO(Sec/SPII): 0.348173
  • Cleavage Site: CS pos: 21-22. AVV-CL. Pr: 0.2409
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15927024 NCBI
• RefSeq: NP_374557 NCBI
• UniProt: A0A0H3JKZ7 UniProt

⊟Protein sequence

• MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSKTNAEKVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMMKIHDMHMN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications