PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_01643
pan locus tag^?: SAUPAN004123000
symbol: SAOUHSC_01643
pan gene symbol^?: comGA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_01643
• symbol: SAOUHSC_01643
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1559316..1560290
• length: 975
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 3920095 NCBI
• RefSeq: YP_500155 NCBI
• BioCyc: G1I0R-1528 BioCyc
• MicrobesOnline: 1290069 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  301
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  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTA
  CATTTTATTCCAGTTAAAAATGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAG
  CAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTATTAGTTTATATGAAGTTTCAA
  GCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
  AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGT
  GTTATCAGAATTGTACCTCAATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGAT
  TTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGTTTAGTGGACCAACTGGTTCA
  GGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
  GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTG
  AATGATAAAGCTGGCATAAACTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCT
  GATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTGCCAAGTGTGTTATACAGGCT
  AGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
  TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATT
  ATAAACCAACGACTTGTAACTACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTA
  TCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATTCATTACCATCATCATTTAAG
  AACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
  CATAAATACATTTAA
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⊟Protein

⊟General

• locus tag: SAOUHSC_01643
• symbol: SAOUHSC_01643
• description: hypothetical protein
• length: 324
• theoretical pI: 9.07987
• theoretical MW: 36864.8
• GRAVY: -0.0317901

⊟Function

• ⊞TIGRFAM:
  Cellular processes Cellular processes Pathogenesis type II secretion system protein E (TIGR02533; HMM-score: 221.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein E (TIGR02533; HMM-score: 221.8)
  Cell structure Cell envelope Surface structures type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
  and 14 more
  Cell structure Cell envelope Surface structures twitching motility protein (TIGR01420; HMM-score: 146.5)
  Cellular processes Cellular processes Chemotaxis and motility twitching motility protein (TIGR01420; HMM-score: 146.5)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid transfer ATPase TraJ (TIGR02525; HMM-score: 84.9)
  Dot/Icm secretion system ATPase DotB (TIGR02524; HMM-score: 58.2)
  Cellular processes Cellular processes Conjugation P-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 57.5)
  P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 54.5)
  helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 35.9)
  Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AA (TIGR02858; HMM-score: 17.1)
  phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 14.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 14.4)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 14.1)
  Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 12.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 12.8)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 12.2)
• ⊞TheSEED  :
  • Late competence protein ComGA, access of DNA to ComEA
  DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComGA, access of DNA to ComEA
  and 1 more
  DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComGA, access of DNA to ComEA
• ⊞PFAM:
  P-loop_NTPase (CL0023) T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 251.4)
  and 21 more
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 25.7)
  nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 19.7)
  Rad17; Rad17 P-loop domain (PF03215; HMM-score: 18.7)
  DAP3; Mitochondrial ribosomal death-associated protein 3 (PF10236; HMM-score: 18.2)
  AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 18)
  AAA_14; AAA domain (PF13173; HMM-score: 17.6)
  NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 17.2)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.3)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16.2)
  AAA_23; AAA domain (PF13476; HMM-score: 15.9)
  AAA_22; AAA domain (PF13401; HMM-score: 15.2)
  ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 15.1)
  AAA_30; AAA domain (PF13604; HMM-score: 14.9)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 14.4)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 14.4)
  G-alpha; G-protein alpha subunit (PF00503; HMM-score: 14)
  NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.2)
  cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 13.2)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 13)
  MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.5)
  AAA_25; AAA domain (PF13481; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.89
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.6324
  • Cytoplasmic Membrane Score: 0.3537
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0139
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003912
  • TAT(Tat/SPI): 0.000073
  • LIPO(Sec/SPII): 0.000327
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 88195351 NCBI
• RefSeq: YP_500155 NCBI
• UniProt: Q2FY30 UniProt
• STRING: 93061.SAOUHSC_01643 STRING

⊟Protein sequence

• MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLNVISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAOUHSC_01639 < SAOUHSC_01640 < SAOUHSC_01641 < SAOUHSC_01643

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications