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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1283 [new locus tag: NWMN_RS07230 ]
  • pan locus tag?: SAUPAN003766000
  • symbol: trpF
  • pan gene symbol?: trpF
  • synonym:
  • product: N-(5'-phosphoribosyl)anthranilate isomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1283 [new locus tag: NWMN_RS07230 ]
  • symbol: trpF
  • product: N-(5'-phosphoribosyl)anthranilate isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 1414146..1414781
  • length: 636
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGATGAAATTGAAATTTTGTGGCTTTACATCAATAAAGGATGTTACAGCGGCCAGTCAA
    TTACCTATTGATGCGATAGGTTTCATCCATTATGAAAAAAGTAAAAGGCATCAAACAATT
    ACCCAAATAAAAAAGTTAGCGTCTGCTGTTCCAAATCATATCGATAAAGTATGTGTCATG
    GTAAATCCTGATTTAACAACAATTGAACACGTATTAAGCAATACGTCAATTAACACAATA
    CAGTTACACGGCACAGAATCTATTGATTTTATACAGGAAATTAAAAAGAAATATTCAAGC
    ATTAAAATCACTAAAGCTTTAGCTGCAGATGAAAACATAATCCAAAACATAAATAAATAT
    AAAGGGTTCGTAGATTTATTTATTATCGACACACCCTCAGTGTCGTATGGTGGTACCGGT
    CAAACATATGACTGGACTATTTTGAAGCACATAAAAGACATACCTTATTTGATAGCAGGA
    GGCATTAACTCTGAAAATATTCAAACAGTTAATCAACTTAAATTATCACATCAAGGTTAT
    GATCTTGCATCAGGTATAGAAGTAAATGGGCGAAAAGATATAGAAAAAATGACAGCAATT
    GTAAATATTGTGAAAGGAGATAGAGACAATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    636

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1283 [new locus tag: NWMN_RS07230 ]
  • symbol: TrpF
  • description: N-(5'-phosphoribosyl)anthranilate isomerase
  • length: 211
  • theoretical pI: 8.22155
  • theoretical MW: 23519.9
  • GRAVY: -0.191469

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 5.3.1.24?  ExPASy
    Phosphoribosylanthranilate isomerase N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
  • TIGRFAM:
    His-Xaa-Ser system putative quinone modification maturase (TIGR03981; HMM-score: 12.3)
  • ⊞TheSEED  :
    • Phosphoribosylanthranilate isomerase (EC 5.3.1.24)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Tryptophan synthesis  Phosphoribosylanthranilate isomerase (EC 5.3.1.24)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Phosphoribosylanthranilate isomerase (EC 5.3.1.24)
  • ⊞PFAM:
    TIM_barrel (CL0036) PRAI; N-(5'phosphoribosyl)anthranilate (PRA) isomerase (PF00697; HMM-score: 130)
    and 3 more
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 14.1)
    no clan defined DUF1995; Domain of unknown function (DUF1995) (PF09353; HMM-score: 13.3)
    SAM (CL0003) SAM_EPH; Ephrin receptor 1 SAM domain-like (PF22993; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9967
    • Cytoplasmic Membrane Score: 0.0013
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0018
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.67
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010438
    • TAT(Tat/SPI): 0.000629
    • LIPO(Sec/SPII): 0.001046
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKKLASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKITKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGGINSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulators: T-box(Trp) (transcription antitermination) regulon, CodY (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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