From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08665 [old locus tag: SACOL1699 ]
  • pan locus tag?: SAUPAN004250000
  • symbol: SACOL_RS08665
  • pan gene symbol?: obgE
  • synonym:
  • product: GTPase ObgE

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS08665 [old locus tag: SACOL1699 ]
  • symbol: SACOL_RS08665
  • product: GTPase ObgE
  • replicon: chromosome
  • strand: -
  • coordinates: 1730884..1732176
  • length: 1293
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1730884..1732176) NCBI
  • BioCyc: SACOL_RS08665 BioCyc
  • MicrobesOnline: see SACOL1699

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTTTGTCGATCAAGTCAAAATATCTCTTAAAGCCGGTGATGGTGGTAATGGTATTACC
    GCATACAGAAGAGAAAAATATGTACCATTTGGTGGACCAGCTGGCGGTGACGGTGGTAAA
    GGTGCTTCAGTCGTATTTGAAGTGGATGAAGGTTTAAGAACGTTATTAGATTTTAGATAT
    CAACGTCATTTTAAAGCAAGCAAAGGTGAAAATGGCCAAAGTAGTAATATGCATGGTAAA
    AATGCGGAAGATTTAGTATTAAAAGTTCCACCTGGTACAATTATTAAAAATGTTGAAACA
    GACGAAGTGTTAGCAGATCTTGTTGAAGATGGTCAAAGAGCTGTAGTAGCGAAGGGCGGT
    CGAGGTGGCCGAGGTAATTCACGTTTTGCAACACCTAGAAACCCTGCACCTGACTTCAGT
    GAAAAAGGTGAACCAGGTGAGGAATTAGATGTATCTTTAGAATTGAAATTATTAGCTGAT
    GTAGGATTAGTAGGTTTCCCTAGTGTGGGTAAATCGACTTTATTATCTATCGTTTCAAAA
    GCTAAGCCTAAAATTGGGGCATATCATTTTACAACGATTAAACCAAATCTAGGTGTTGTT
    TCAACGCCTGATCAACGTAGTTTTGTTATGGCAGATTTACCAGGTTTAATTGAAGGTGCA
    TCTGATGGCGTTGGATTAGGACATCAATTTTTAAGACATGTAGAGAGAACAAAAGTTATT
    GTTCACATGATTGATATGAGCGGTTCTGAAGGTAGAGAACCTATTGAAGATTATAAAGTC
    ATTAATCAAGAATTAGCTGCGTACGAGCAACGTTTAGAAGATAGACCTCAAATCGTAGTA
    GCTAACAAGATGGATTTACCTGAATCACAAGATAATTTAAACTTGTTTAAAGAAGAAATT
    GGCGAAGATGTGCCAGTTATTCCAGTTTCAACAATAACGCGTGATAATATTGATCAATTA
    TTATATGCAATAGCAGATAAATTAGAAGAATATAAAGATGTTGACATCACAGTTGAAGAA
    GAGGAGTCAGTTGGCATTAACCGAGTATTATATAAACATACACCGTCACAAGATAAATTT
    ACAATTTCAAGAGATGATGATGGTGCTTATGTGGTAAGTGGTAATGCTATTGAAAGAATG
    TTTAAAATGACTGACTTTAACAGTGATCCAGCAGTACGTCGATTTGCTCGTCAAATGCGT
    TCGATGGGTATTGATGATGCGCTTAGAGAACGTGGTTGTAAAAATGGTGATATCGTTAGA
    ATTCTTGGCGGAGAATTTGAATTCGTTGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1293

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS08665 [old locus tag: SACOL1699 ]
  • symbol: SACOL_RS08665
  • description: GTPase ObgE
  • length: 430
  • theoretical pI: 4.79119
  • theoretical MW: 47200.9
  • GRAVY: -0.409535

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.5.-?  ExPASy
  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 467.3)
    and 15 more
    Obg family GTPase CgtA, C-terminal extension (TIGR03595; HMM-score: 92.8)
    Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 61.9)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 51.8)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 42.1)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 38.6)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 33.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 24.3)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 21.6)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 21.6)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 21.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 13.7)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 13.4)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage tail component, N-terminal domain (TIGR01633; HMM-score: 12.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.7)
    Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.7)
  • TheSEED: see SACOL1699
  • ⊞PFAM:
    GlyR (CL0762) GTP1_OBG; GTP1/OBG (PF01018; HMM-score: 215.9)
    and 11 more
    no clan defined DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 89.4)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 86.4)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 38.3)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 20.6)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 17.5)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 16.5)
    P-loop_NTPase (CL0023) Arf; ADP-ribosylation factor family (PF00025; HMM-score: 16.1)
    Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 16)
    no clan defined DUF3145; Protein of unknown function (DUF3145) (PF11343; HMM-score: 13.5)
    P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 13.1)
    NOG1; Nucleolar GTP-binding protein 1 (NOG1) (PF06858; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9839
    • Cytoplasmic Membrane Score: 0.003
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.013
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015559
    • TAT(Tat/SPI): 0.001934
    • LIPO(Sec/SPII): 0.002154
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDITVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1699
  • protein localization: see SACOL1699
  • quantitative data / protein copy number per cell: see SACOL1699
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS08665&oldid=99666"