PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08665 [old locus tag: SACOL1699 ]
• pan locus tag^?: SAUPAN004250000
• symbol: SACOL_RS08665
• pan gene symbol^?: obgE
• synonym:
• product: GTPase ObgE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08665 [old locus tag: SACOL1699 ]
• symbol: SACOL_RS08665
• product: GTPase ObgE
• replicon: chromosome
• strand: -
• coordinates: 1730884..1732176
• length: 1293
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1730884..1732176) NCBI
• BioCyc: SACOL_RS08665 BioCyc
• MicrobesOnline: see SACOL1699

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTTGTCGATCAAGTCAAAATATCTCTTAAAGCCGGTGATGGTGGTAATGGTATTACC
  GCATACAGAAGAGAAAAATATGTACCATTTGGTGGACCAGCTGGCGGTGACGGTGGTAAA
  GGTGCTTCAGTCGTATTTGAAGTGGATGAAGGTTTAAGAACGTTATTAGATTTTAGATAT
  CAACGTCATTTTAAAGCAAGCAAAGGTGAAAATGGCCAAAGTAGTAATATGCATGGTAAA
  AATGCGGAAGATTTAGTATTAAAAGTTCCACCTGGTACAATTATTAAAAATGTTGAAACA
  GACGAAGTGTTAGCAGATCTTGTTGAAGATGGTCAAAGAGCTGTAGTAGCGAAGGGCGGT
  CGAGGTGGCCGAGGTAATTCACGTTTTGCAACACCTAGAAACCCTGCACCTGACTTCAGT
  GAAAAAGGTGAACCAGGTGAGGAATTAGATGTATCTTTAGAATTGAAATTATTAGCTGAT
  GTAGGATTAGTAGGTTTCCCTAGTGTGGGTAAATCGACTTTATTATCTATCGTTTCAAAA
  GCTAAGCCTAAAATTGGGGCATATCATTTTACAACGATTAAACCAAATCTAGGTGTTGTT
  TCAACGCCTGATCAACGTAGTTTTGTTATGGCAGATTTACCAGGTTTAATTGAAGGTGCA
  TCTGATGGCGTTGGATTAGGACATCAATTTTTAAGACATGTAGAGAGAACAAAAGTTATT
  GTTCACATGATTGATATGAGCGGTTCTGAAGGTAGAGAACCTATTGAAGATTATAAAGTC
  ATTAATCAAGAATTAGCTGCGTACGAGCAACGTTTAGAAGATAGACCTCAAATCGTAGTA
  GCTAACAAGATGGATTTACCTGAATCACAAGATAATTTAAACTTGTTTAAAGAAGAAATT
  GGCGAAGATGTGCCAGTTATTCCAGTTTCAACAATAACGCGTGATAATATTGATCAATTA
  TTATATGCAATAGCAGATAAATTAGAAGAATATAAAGATGTTGACATCACAGTTGAAGAA
  GAGGAGTCAGTTGGCATTAACCGAGTATTATATAAACATACACCGTCACAAGATAAATTT
  ACAATTTCAAGAGATGATGATGGTGCTTATGTGGTAAGTGGTAATGCTATTGAAAGAATG
  TTTAAAATGACTGACTTTAACAGTGATCCAGCAGTACGTCGATTTGCTCGTCAAATGCGT
  TCGATGGGTATTGATGATGCGCTTAGAGAACGTGGTTGTAAAAATGGTGATATCGTTAGA
  ATTCTTGGCGGAGAATTTGAATTCGTTGAATAG
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⊟Protein

⊟General

• locus tag: SACOL_RS08665 [old locus tag: SACOL1699 ]
• symbol: SACOL_RS08665
• description: GTPase ObgE
• length: 430
• theoretical pI: 4.79119
• theoretical MW: 47200.9
• GRAVY: -0.409535

⊟Function

• reaction:
  EC 3.6.5.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 467.3)
  and 15 more
  Obg family GTPase CgtA, C-terminal extension (TIGR03595; HMM-score: 92.8)
  Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 61.9)
  Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 51.8)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 42.1)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 38.6)
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 33.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 24.3)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 21.6)
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 21.6)
  translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 21.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 13.7)
  Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 13.4)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage tail component, N-terminal domain (TIGR01633; HMM-score: 12.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.7)
  Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.7)
• TheSEED: see SACOL1699
• PFAM:
  no clan defined GTP1_OBG; GTP1/OBG (PF01018; HMM-score: 203.8)
  and 13 more
  DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 89.4)
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 83.2)
  FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 40.1)
  Arf; ADP-ribosylation factor family (PF00025; HMM-score: 17.3)
  Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 16.9)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 16.5)
  ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 16.4)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 15.1)
  ABC_tran; ABC transporter (PF00005; HMM-score: 14.1)
  no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 13.8)
  DUF3145; Protein of unknown function (DUF3145) (PF11343; HMM-score: 13.6)
  P-loop_NTPase (CL0023) MMR_HSR1_C; GTPase of unknown function C-terminal (PF08438; HMM-score: 13.5)
  NOG1; Nucleolar GTP-binding protein 1 (NOG1) (PF06858; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.015559
  • TAT(Tat/SPI): 0.001934
  • LIPO(Sec/SPII): 0.002154
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446418247 NCBI
• RefSeq: WP_000496102 NCBI
• UniProt: see SACOL1699

⊟Protein sequence

• MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDITVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE

⊟Experimental data

• experimentally validated: see SACOL1699
• protein localization: see SACOL1699
• quantitative data / protein copy number per cell: see SACOL1699
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications