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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01753
  • pan locus tag?: SAUPAN004250000
  • symbol: obgE
  • pan gene symbol?: obgE
  • synonym:
  • product: GTPase ObgE

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01753
  • symbol: obgE
  • product: GTPase ObgE
  • replicon: chromosome
  • strand: -
  • coordinates: 1656924..1658216
  • length: 1293
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTTTGTCGATCAAGTCAAAATATCTCTTAAAGCCGGTGATGGTGGTAATGGTATTACC
    GCATACAGAAGAGAAAAATATGTACCATTTGGTGGACCAGCTGGCGGTGACGGTGGTAAA
    GGTGCTTCAGTCGTATTTGAAGTGGATGAAGGTTTAAGAACGTTATTAGATTTTAGATAT
    CAACGTCATTTTAAAGCAAGCAAAGGTGAAAATGGCCAAAGTAGTAATATGCATGGTAAA
    AATGCGGAAGATTTAGTATTAAAAGTTCCACCTGGTACAATTATTAAAAATGTTGAAACA
    GACGAAGTGTTAGCAGATCTTGTTGAAGATGGTCAAAGAGCTGTAGTAGCGAAGGGCGGT
    CGAGGTGGCCGAGGTAATTCACGTTTTGCAACACCTAGAAACCCTGCACCTGACTTCAGT
    GAAAAAGGTGAACCAGGTGAGGAATTAGATGTATCTTTAGAATTGAAATTATTAGCTGAT
    GTAGGATTAGTAGGTTTCCCTAGTGTGGGTAAATCGACTTTATTATCTATCGTTTCAAAA
    GCTAAGCCTAAAATTGGGGCATATCATTTTACAACGATTAAACCAAATCTAGGTGTTGTT
    TCAACGCCTGATCAACGTAGTTTTGTTATGGCAGATTTACCAGGTTTAATTGAAGGTGCA
    TCTGATGGCGTTGGATTAGGACATCAATTTTTAAGACATGTAGAGAGAACAAAAGTTATT
    GTTCACATGATTGATATGAGCGGTTCTGAAGGTAGAGAACCTATTGAAGATTATAAAGTC
    ATTAATCAAGAATTAGCTGCGTACGAGCAACGTTTAGAAGATAGACCTCAAATCGTAGTA
    GCTAACAAGATGGATTTACCTGAATCACAAGATAATTTAAACTTGTTTAAAGAAGAAATT
    GGCGAAGATGTGCCAGTTATTCCAGTTTCAACAATAACGCGTGATAATATTGATCAATTA
    TTATATGCAATAGCAGATAAATTAGAAGAATATAAAGATGTTGACTTCACAGTTGAAGAA
    GAGGAGTCAGTTGGCATTAACCGAGTATTATATAAACATACACCGTCACAAGATAAATTT
    ACAATTTCAAGAGATGATGATGGTGCTTATGTGGTAAGTGGTAATGCTATTGAAAGAATG
    TTTAAAATGACTGACTTTAACAGTGATCCAGCAGTACGTCGATTTGCTCGTCAAATGCGT
    TCGATGGGTATTGATGATGCGCTTAGAGAACGTGGTTGTAAAAATGGTGATATCGTTAGA
    ATTCTTGGCGGAGAATTTGAATTCGTTGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1293

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01753
  • symbol: ObgE
  • description: GTPase ObgE
  • length: 430
  • theoretical pI: 4.79119
  • theoretical MW: 47234.9
  • GRAVY: -0.413488

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.5.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 467.3)
    and 14 more
    Obg family GTPase CgtA, C-terminal extension (TIGR03595; HMM-score: 92.8)
    Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 62)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 51.8)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 42.1)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 40.2)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 33)
    Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 24.1)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 21.6)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 21.6)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 21.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 13.6)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 13.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.9)
    Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.9)
  • TheSEED  :
    • GTP-binding protein Obg
    Protein Metabolism Protein biosynthesis Universal GTPases  GTP-binding protein Obg
  • PFAM:
    no clan defined GTP1_OBG; GTP1/OBG (PF01018; HMM-score: 203.8)
    and 13 more
    DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 89.5)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 83.1)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 40.1)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 17.3)
    no clan defined DUF3145; Protein of unknown function (DUF3145) (PF11343; HMM-score: 17.1)
    P-loop_NTPase (CL0023) Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 16.8)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 16.6)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 16.5)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 15.1)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.4)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 13.8)
    P-loop_NTPase (CL0023) MMR_HSR1_C; GTPase of unknown function C-terminal (PF08438; HMM-score: 13.6)
    NOG1; Nucleolar GTP-binding protein 1 (NOG1) (PF06858; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015559
    • TAT(Tat/SPI): 0.001934
    • LIPO(Sec/SPII): 0.002154
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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