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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01095 [old locus tag: SA0183 ]
  • pan locus tag?: SAUPAN001032000
  • symbol: SA_RS01095
  • pan gene symbol?: glcA
  • synonym:
  • product: PTS glucose EIICBA component

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS01095 [old locus tag: SA0183 ]
  • symbol: SA_RS01095
  • product: PTS glucose EIICBA component
  • replicon: chromosome
  • strand: -
  • coordinates: 214814..216859
  • length: 2046
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (214814..216859) NCBI
  • BioCyc: SA_RS01095 BioCyc
  • MicrobesOnline: see SA0183

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    GTGAGGAAGAAACTTTTCGGTCAATTGCAACGTATTGGTAAAGCGCTAATGTTACCTGTT
    GCAATTTTACCAGCAGCGGGTCTGTTATTAGCTATCGGTACAGCTATACAAGGCGAAGCA
    TTACAACACTACTTGCCGTTTATACAAAATGGTGGCGTTCAAAATGTCGCTAAATTAATG
    ACAGCTGCCGGTAGTATTATTTTTGAAAACTTACCTATGATTTTCGCATTAGGTGTCGCA
    ATCGGATTAGCTGGCGGTGATGGCGTAGCAGCTATCGCAGCATTCGTCGGTTACATAATC
    ATGAACAAAACAATGGGCGACTTTTTACAAGTTACACCTAAAAATGTTACTGATCCAGCG
    AGTGGTTACGCTAGCATTTTAGGTATTCCAACATTACAAACAGGTGTGTTCGGCGGTATT
    ATTATCGGGGCCCTGGCAGCTTGGTGTTATAACAAGTTCTATAACATTAACTTACCATCT
    TATTTAGGTTTCTTCGCTGGTAAGCGTTTCGTACCTATTATGATGGCTACAACATCATTT
    ATTTTAGCATTTCCAATGGCATTAATTTGGCCAACGATTCAATCAGGATTAAATGCATTC
    AGTACAGGATTATTAGATTCAAATACTGGTGTTGCCGTATTCTTATTTGGTTTCATCAAG
    CGTTTATTAATTCCATTCGGTCTACATCACATTTTCCACGCACCGTTCTGGTTCGAGTTT
    GGTTCATGGAAAAATGCAGCTGGTGAAATTATTCACGGTGACCAACGTATCTTTATCGAA
    CAAATTCGTGAAGGCGCACATTTGACAGCTGGTAAATTCATGCAAGGTGAATTCCCTGTT
    ATGATGTTCGGTTTACCTGCAGCAGCTTTAGCAATTTATCACACAGCTAAACCTGAAAAT
    AAGAAAGTAGTAGCAGGTTTAATGGGTTCTGCTGCTTTAACATCATTCTTAACTGGTATT
    ACAGAACCTTTAGAATTCTCATTCTTATTCGTAGCACCATTATTATTCTTTATTCACGCA
    GTACTTGATGGTTTATCATTCTTAACATTGTACTTATTGGATGTTCACTTAGGTTATACT
    TTCTCAGGTGGTTTCATCGACTACGTTTTACTTGGCGTACTACCTAATAAGACACAATGG
    TGGTTAGTCATTCCTGTAGGTCTTGTATACGCAGTTATTTACTACTTCGTATTCCGATTC
    TTAATTGTAAAATTAAAATACAAAACACCAGGTCGTGAAGATAAGCAATCACAAGCTGTT
    ACTGCTTCAGCAACTGAATTACCATATGCAGTATTAGAAGCTATGGGTGGCAAAGCAAAC
    ATTAAACATTTAGACGCTTGTATCACACGTCTACGTGTTGAAGTTAACGACAAATCTAAA
    GTTGATGTTCCTGGTTTGAAAGATTTAGGCGCATCTGGTGTATTAGAAGTCGGCAATAAT
    ATGCAAGCAATTTTTGGTCCTAAATCTGACCAAATCAAACATGAAATGCAACAGATTATG
    AATGGTCAAGTAGTAGAAAACCCTACTACTATGGAAGACGATAAAGACGAAACTGTTGTT
    GTTGCAGAAGATAAATCTGCAACAAGCGAATTGAGCCATATCGTTCATGCACCATTAACT
    GGTGAAGTAACACCTTTATCAGAAGTGCCTGATCAAGTGTTCAGCGAAAAAATGATGGGT
    GACGGTATCGCTATTAAACCTTCACAAGGTGAAGTTCGTGCACCATTCAACGGTAAAATA
    CAAATGATTTTCCCAACAAAACATGCAATTGGTCTTGTATCAGATAGTGGTTTAGAACTA
    TTAATCCACATCGGTTTAGACACTGTTAAATTAAACGGAGAAGGCTTTACTTTACATGTT
    GAGGAAGGTCAAGAAGTTAAACAAGGTGATTTATTAATCAACTTTGATTTAGACTACATC
    CGCAATCATGCAAAGAGTGATATTACGCCTATTATCGTGACACAAGGAAACATTACAAAC
    CTTGATTTTAAACAAGGTGAACATGGCAACATTTCATTTGGCGATCAATTATTTGAAGCT
    AAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2046

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS01095 [old locus tag: SA0183 ]
  • symbol: SA_RS01095
  • description: PTS glucose EIICBA component
  • length: 681
  • theoretical pI: 6.33084
  • theoretical MW: 73957.7
  • GRAVY: 0.330837

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.1.199?  ExPASy
    Protein-N(pi)-phosphohistidine--D-glucose phosphotransferase [Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 799.9)
    and 15 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 478.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 401.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 286.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 278.7)
    Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 278.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 243.9)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 210)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.8)
    Signal transduction Signal transduction PTS PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 62.6)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 16.1)
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 14.6)
  • TheSEED: see SA0183
  • ⊞PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 321.1)
    and 5 more
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 183.3)
    no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 57.5)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 20.8)
    no clan defined PIG-P; PIG-P (PF08510; HMM-score: 14.7)
    RDD; RDD family (PF06271; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0003
    • Cytoplasmic Membrane Score: 0.999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0006
  • LocateP:
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 38 aa
    • SP(Sec/SPI): 0.565966
    • TAT(Tat/SPI): 0.021723
    • LIPO(Sec/SPII): 0.044643
    • Cleavage Site: CS pos: 38-39. IQG-EA. Pr: 0.2232
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

  • GI: 447150453 NCBI
  • RefSeq: WP_001227709 NCBI
  • UniProt: see SA0183

⊟Protein sequence[edit | edit source]

  • MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHYLPFIQNGGVQNVAKLMTAAGSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMNKTMGDFLQVTPKNVTDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPSYLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGFIKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQGEFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLTLYLLDVHLGYTFSGGFIDYVLLGVLPNKTQWWLVIPVGLVYAVIYYFVFRFLIVKLKYKTPGREDKQSQAVTASATELPYAVLEAMGGKANIKHLDACITRLRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENPTTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKIQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA see SA0183

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS01095&oldid=85226"
  • This page was last edited on 11 March 2016, at 05:11.
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