From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0175 [new locus tag: SACOL_RS00890 ]
  • pan locus tag?: SAUPAN001032000
  • symbol: SACOL0175
  • pan gene symbol?: glcA
  • synonym:
  • product: PTS system, IIABC components

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0175 [new locus tag: SACOL_RS00890 ]
  • symbol: SACOL0175
  • product: PTS system, IIABC components
  • replicon: chromosome
  • strand: -
  • coordinates: 200576..202621
  • length: 2046
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    GTGAGGAAGAAACTTTTCGGTCAATTGCAACGTATTGGTAAAGCGCTAATGTTACCTGTT
    GCGATTTTACCAGCAGCTGGTCTGTTATTAGCTATCGGTACAGCTATGCAAGGTGAATCA
    TTACAACACTACTTGCCGTTTATACAAAATGGTGGCGTACAAACTGTCGCTAAATTAATG
    ACAGGTGCTGGTGGTATCATTTTTGATAACTTGCCTATGATTTTCGCATTAGGTGTCGCA
    ATCGGATTAGCTGGCGGTGATGGCGTAGCAGCTATCGCAGCATTCGTCGGTTACATAATC
    ATGAACAAAACAATGGGCGACTTTTTACAAGTTACACCTAAGAATATTGGTGATCCAGCG
    AGTGGTTACGCTAGCATTTTAGGTATCCCAACATTACAAACAGGTGTGTTCGGCGGTATT
    ATAATCGGGGCCCTGGCAGCTTGGTGTTATAACAAGTTCTATAACATTAACTTACCATCT
    TATTTAGGTTTCTTCGCTGGTAAGCGTTTCGTACCTATTATGATGGCTACAACATCATTT
    ATTTTAGCATTCCCAATGGCATTAATTTGGCCAACGATTCAATCAGGATTAAATGCATTC
    AGTACAGGATTATTAGATTCAAATACTGGTGTTGCCGTATTCTTATTTGGTTTCATCAAG
    CGTTTATTAATTCCATTCGGTCTACATCACATTTTCCACGCACCGTTCTGGTTCGAGTTT
    GGTTCATGGAAAAATGCAGCTGGTGAAATTATTCACGGTGACCAACGTATCTTTATCGAA
    CAAATTCGTGAAGGCGCACATTTGACAGCTGGTAAATTCATGCAAGGTGAATTCCCTGTT
    ATGATGTTCGGTTTACCTGCAGCAGCTTTAGCAATTTATCACACAGCTAAACCTGAAAAT
    AAGAAAGTAGTAGCAGGTTTAATGGGTTCTGCTGCTTTAACATCATTCTTAACTGGTATT
    ACAGAACCATTAGAATTCTCATTCTTATTTGTAGCACCATTATTATTCTTTATTCACGCA
    GTACTTGATGGTTTATCATTCTTAACATTGTACTTATTAGATCTTCATCTAGGTTATACA
    TTCTCAGGTGGTTTCATCGACTACTTCTTACTCGGTATACTACCTAATAAGACACAATGG
    TGGTTAGTCATTCCTGTAGGTCTTGTATACGCAGTTATTTACTACTTCGTATTCCGATTC
    TTAATTGTAAAATTAAAATACAAAACACCAGGTCGTGAAGATAAACAATCACAAGCGGCT
    ACTGCTTCAGCAACTGAATTACCATATGCAGTATTAGAAGCTATGGGTGGCAAAGCAAAC
    ATTAAACATTTAGACGCTTGTATCACACGTCTACGTGTTGAAGTTAACGACAAATCTAAA
    GTTGATGTTCCTGGTTTGAAAGATTTAGGCGCATCTGGTGTATTAGAAGTCGGCAATAAT
    ATGCAAGCAATTTTTGGTCCTAAATCTGACCAAATCAAACATGAAATGCAACAGATTATG
    AATGGTCAAGTAGTAGAAAATCCTACTACTATGGAAGACGATAAAGACGAAACTGTTGTT
    GTTGCAGAAGATAAATCTGCAACAAGCGAATTGAGCCATATCGTGCATGCACCATTAACT
    GGTGAAGTAACACCATTATCAGAAGTGCCTGATCAAGTGTTCAGCGAAAAAATGATGGGT
    GACGGTATCGCTATCAAACCTTCACAAGGTGAAGTTCGTGCACCATTCAACGGTAAAGTA
    CAAATGATTTTCCCAACAAAACATGCAATTGGTCTTGTATCAGATAGTGGTTTAGAACTA
    TTAATCCACATCGGTTTAGACACTGTTAAATTAAACGGAGAAGGCTTTACTTTACATGTT
    GAGGAAGGTCAAGAAGTTAAACAAGGTGATTTATTAATCAACTTTGATTTAGACTACATC
    CGCAATCATGCAAAGAGTGATATTACGCCTATTATCGTGACACAAGGAAACATTACAAAC
    CTTGATTTTAAACAAGGTGAACATGGCAACATTTCATTTGGCGATCAATTATTTGAAGCT
    AAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2046

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0175 [new locus tag: SACOL_RS00890 ]
  • symbol: SACOL0175
  • description: PTS system, IIABC components
  • length: 681
  • theoretical pI: 6.33064
  • theoretical MW: 73924.6
  • GRAVY: 0.319383

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.199?  ExPASy
    Protein-N(pi)-phosphohistidine--D-glucose phosphotransferase [Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 800.6)
    and 15 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 477.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 396.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 284)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 279.2)
    Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 279.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 243.6)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 209.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.4)
    Signal transduction Signal transduction PTS PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.8)
    Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.8)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 57)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 16.5)
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 14.2)
  • TheSEED  :
    • PTS system, glucose-specific IIA component
    • PTS system, glucose-specific IIB component (EC 2.7.1.69)
    • PTS system, glucose-specific IIC component
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  PTS system, glucose-specific IIA component
    and 2 more
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  PTS system, glucose-specific IIB component (EC 2.7.1.69)
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  PTS system, glucose-specific IIC component
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 328.1)
    and 5 more
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 181.7)
    no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 53)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 20.1)
    no clan defined PIG-P; PIG-P (PF08510; HMM-score: 14.9)
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 38 aa
    • SP(Sec/SPI): 0.573742
    • TAT(Tat/SPI): 0.028485
    • LIPO(Sec/SPII): 0.075145
    • Cleavage Site: CS pos: 38-39. MQG-ES. Pr: 0.2044
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHYLPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMNKTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPSYLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGFIKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQGEFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAVIYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITRLRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENPTTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL0175&oldid=97688"