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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0183 [new locus tag: SA_RS01095 ]
- pan locus tag?: SAUPAN001032000
- symbol: glcA
- pan gene symbol?: glcA
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0183 [new locus tag: SA_RS01095 ]
- symbol: glcA
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 214814..216859
- length: 2046
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1122959 NCBI
- RefSeq: NP_373426 NCBI
- BioCyc: see SA_RS01095
- MicrobesOnline: 102452 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2041GTGAGGAAGAAACTTTTCGGTCAATTGCAACGTATTGGTAAAGCGCTAATGTTACCTGTT
GCAATTTTACCAGCAGCGGGTCTGTTATTAGCTATCGGTACAGCTATACAAGGCGAAGCA
TTACAACACTACTTGCCGTTTATACAAAATGGTGGCGTTCAAAATGTCGCTAAATTAATG
ACAGCTGCCGGTAGTATTATTTTTGAAAACTTACCTATGATTTTCGCATTAGGTGTCGCA
ATCGGATTAGCTGGCGGTGATGGCGTAGCAGCTATCGCAGCATTCGTCGGTTACATAATC
ATGAACAAAACAATGGGCGACTTTTTACAAGTTACACCTAAAAATGTTACTGATCCAGCG
AGTGGTTACGCTAGCATTTTAGGTATTCCAACATTACAAACAGGTGTGTTCGGCGGTATT
ATTATCGGGGCCCTGGCAGCTTGGTGTTATAACAAGTTCTATAACATTAACTTACCATCT
TATTTAGGTTTCTTCGCTGGTAAGCGTTTCGTACCTATTATGATGGCTACAACATCATTT
ATTTTAGCATTTCCAATGGCATTAATTTGGCCAACGATTCAATCAGGATTAAATGCATTC
AGTACAGGATTATTAGATTCAAATACTGGTGTTGCCGTATTCTTATTTGGTTTCATCAAG
CGTTTATTAATTCCATTCGGTCTACATCACATTTTCCACGCACCGTTCTGGTTCGAGTTT
GGTTCATGGAAAAATGCAGCTGGTGAAATTATTCACGGTGACCAACGTATCTTTATCGAA
CAAATTCGTGAAGGCGCACATTTGACAGCTGGTAAATTCATGCAAGGTGAATTCCCTGTT
ATGATGTTCGGTTTACCTGCAGCAGCTTTAGCAATTTATCACACAGCTAAACCTGAAAAT
AAGAAAGTAGTAGCAGGTTTAATGGGTTCTGCTGCTTTAACATCATTCTTAACTGGTATT
ACAGAACCTTTAGAATTCTCATTCTTATTCGTAGCACCATTATTATTCTTTATTCACGCA
GTACTTGATGGTTTATCATTCTTAACATTGTACTTATTGGATGTTCACTTAGGTTATACT
TTCTCAGGTGGTTTCATCGACTACGTTTTACTTGGCGTACTACCTAATAAGACACAATGG
TGGTTAGTCATTCCTGTAGGTCTTGTATACGCAGTTATTTACTACTTCGTATTCCGATTC
TTAATTGTAAAATTAAAATACAAAACACCAGGTCGTGAAGATAAGCAATCACAAGCTGTT
ACTGCTTCAGCAACTGAATTACCATATGCAGTATTAGAAGCTATGGGTGGCAAAGCAAAC
ATTAAACATTTAGACGCTTGTATCACACGTCTACGTGTTGAAGTTAACGACAAATCTAAA
GTTGATGTTCCTGGTTTGAAAGATTTAGGCGCATCTGGTGTATTAGAAGTCGGCAATAAT
ATGCAAGCAATTTTTGGTCCTAAATCTGACCAAATCAAACATGAAATGCAACAGATTATG
AATGGTCAAGTAGTAGAAAACCCTACTACTATGGAAGACGATAAAGACGAAACTGTTGTT
GTTGCAGAAGATAAATCTGCAACAAGCGAATTGAGCCATATCGTTCATGCACCATTAACT
GGTGAAGTAACACCTTTATCAGAAGTGCCTGATCAAGTGTTCAGCGAAAAAATGATGGGT
GACGGTATCGCTATTAAACCTTCACAAGGTGAAGTTCGTGCACCATTCAACGGTAAAATA
CAAATGATTTTCCCAACAAAACATGCAATTGGTCTTGTATCAGATAGTGGTTTAGAACTA
TTAATCCACATCGGTTTAGACACTGTTAAATTAAACGGAGAAGGCTTTACTTTACATGTT
GAGGAAGGTCAAGAAGTTAAACAAGGTGATTTATTAATCAACTTTGATTTAGACTACATC
CGCAATCATGCAAAGAGTGATATTACGCCTATTATCGTGACACAAGGAAACATTACAAAC
CTTGATTTTAAACAAGGTGAACATGGCAACATTTCATTTGGCGATCAATTATTTGAAGCT
AAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0183 [new locus tag: SA_RS01095 ]
- symbol: GlcA
- description: hypothetical protein
- length: 681
- theoretical pI: 6.33084
- theoretical MW: 73957.7
- GRAVY: 0.330837
⊟Function[edit | edit source]
- reaction: EC 2.7.1.199? ExPASyProtein-N(pi)-phosphohistidine--D-glucose phosphotransferase [Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
- TIGRFAM: Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 799.9)and 15 moreTransport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 478.9)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 401.2)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 286.3)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 278.7)Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 278.7)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 243.9)PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 210)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.8)Signal transduction PTS PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.8)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 62.6)PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28.4)Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 16.1)Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 14.6)
- TheSEED :
- PTS system, glucose-specific IIA component (EC 2.7.1.199)
- PTS system, glucose-specific IIB component (EC 2.7.1.199)
- PTS system, glucose-specific IIC component
Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization PTS system, glucose-specific IIA componentand 2 more - PFAM: PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 329.9)and 4 moreHybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 181.7)no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 53.2)Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 20)no clan defined PIG-P; PIG-P (PF08510; HMM-score: 16.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 10
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide SP(Sec/SPI) length 38 aa
- SP(Sec/SPI): 0.565966
- TAT(Tat/SPI): 0.021723
- LIPO(Sec/SPII): 0.044643
- Cleavage Site: CS pos: 38-39. IQG-EA. Pr: 0.2232
- predicted transmembrane helices (TMHMM): 10
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHYLPFIQNGGVQNVAKLMTAAGSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMNKTMGDFLQVTPKNVTDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPSYLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGFIKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQGEFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLTLYLLDVHLGYTFSGGFIDYVLLGVLPNKTQWWLVIPVGLVYAVIYYFVFRFLIVKLKYKTPGREDKQSQAVTASATELPYAVLEAMGGKANIKHLDACITRLRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENPTTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKIQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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