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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2346 [new locus tag: SA_RS13445 ]
  • pan locus tag?: SAUPAN006223000
  • symbol: SA2346
  • pan gene symbol?: —
  • synonym:
  • product: D-lactate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2346 [new locus tag: SA_RS13445 ]
  • symbol: SA2346
  • product: D-lactate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2635605..2636603
  • length: 999
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAAAAATTAAAATCATGAGTGTACGTGATGAAGATATGCCTTATATCAAGGCGTGG
    GCAGAGAAACATCATGTTGAGGTAGATATTACTAAAGAAGCATTAACTGATGACAATGTT
    GAAGGTGTCGCTGGTTATGATGGCTTATCGCTATCACAACAAATACCATTATCAGAGCAT
    GTTTATAAACGATTAAATGAACTAGGTATTAAACAAATCGCACAACGTAGTGCTGGATTT
    GATACATATGATTTGGAGTTAGCAAACAAGTATAACTTGATTGTGTCAAATGTGCCGTCA
    TACTCTCCGAATTCAATTGCGGAGTTTGCTGTGAACCAGGCTATTAATGTGGTTCGTCAT
    TTTAATCAAATTCAAACGAAAGTGCGGGAGCATGATTTTAGATGGGAACCTACTATTTTA
    TCGAAATCCATTAAAGATTTAAAAGTAGCTGTTATTGGTACAGGTCGAATTGGCCGTGTA
    GTAGCCGATATATTTGCCAATGGTTATCAAAGTGATGTGGTCGCATACGACCCGTTTCCT
    AATGCTAAAATTGCAACGTATGTCGATTACAAAGATACGATTGAGGAAGCGGTTGAAGGT
    GCTGATATCGTGACATTACATGTACCTGCAACGAAATATAATCATTATCTATTTAATGCT
    GAATTATTTAAACATTTTAAAAAGGGCGCAGTATTTGTCAATTGTGCGAGAGGTTCTTTA
    GTAGATACTAAGGCGTTATTAGACGCATTAGACAATGGTGTGATTAAAGGTGCAGCACTT
    GATACGTATGAATTTGAACGCAAACTTTTCCCAAGTGATCAAAGGGGCAAAACACCGAAC
    GATCCATTGTTAGAATCGTTGATTGACAGGGAAGATGTCATATTAACACCACATATTGCG
    TTTTATACTGAAGCTGCAGTTAAAAATCTAATTGTCGATGCATTAGATGCAACATTAGAT
    GTATTGCAGACTGGAGATACTAGGTTACGAGTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2346 [new locus tag: SA_RS13445 ]
  • symbol: SA2346
  • description: D-lactate dehydrogenase
  • length: 332
  • theoretical pI: 5.25933
  • theoretical MW: 37172.9
  • GRAVY: -0.209639

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 1.1.1.28?  ExPASy
    D-lactate dehydrogenase (R)-lactate + NAD+ = pyruvate + NADH
  • ⊞TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 168.9)
    and 6 more
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 18)
    2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 16.7)
    glycerol-3-phosphate dehydrogenase (NAD(+)) (TIGR03376; EC 1.1.1.8; HMM-score: 14)
    Metabolism Energy metabolism Sugars inositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 14)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 12.6)
    Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 12.2)
  • TheSEED  :
    • D-lactate dehydrogenase (EC 1.1.1.28)
    CBSS-196620.1.peg.2477  D-specific D-2-hydroxyacid dehydrogenase ddh homolog (EC 1.1.1.28)
  • ⊞PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 161.7)
    and 14 more
    2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 97)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 29)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 23.6)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 21.7)
    KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 21.1)
    HUP (CL0039) UDPG_MGDP_dh_C; UDP-glucose/GDP-mannose dehydrogenase family, UDP binding domain (PF03720; HMM-score: 19.8)
    NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 19)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 17.7)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 17.3)
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 14.7)
    PglD_N; PglD N-terminal domain (PF17836; HMM-score: 13.7)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13.5)
    no clan defined CRASP-2; Complement regulator-acquiring surface protein 2 (PF22487; HMM-score: 13.3)
    NADP_Rossmann (CL0063) TrkA_N; TrkA-N domain (PF02254; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9966
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0031
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008111
    • TAT(Tat/SPI): 0.000323
    • LIPO(Sec/SPII): 0.000661
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVAGYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYSPNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRVVADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLFNAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRGKTPNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CodY (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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