PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2459 [new locus tag: NWMN_RS14130 ]
• pan locus tag^?: SAUPAN006223000
• symbol: NWMN_2459
• pan gene symbol^?: —
• synonym:
• product: D-lactate dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2459 [new locus tag: NWMN_RS14130 ]
• symbol: NWMN_2459
• product: D-lactate dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2705648..2706646
• length: 999
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331521 NCBI
• RefSeq: YP_001333493 NCBI
• BioCyc:
• MicrobesOnline: 3708061 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACAAAAATTAAAATCATGAGTGTACGTGATGAAGATATGCCTTATATCAAGGCGTGG
  GCAGAGAAACATCATGTTGAGGTAGATATTACTAAAGAAGCATTAACTGATGACAATGTT
  GAAGGTGTCGCTGGTTATGATGGCTTATCGCTATCACAACAAATACCATTATCAGAGCAT
  GTTTATAAACGATTAAATGAACTAGGTATTAAACAAATCGCACAACGTAGTGCTGGATTT
  GATACATATGATTTGGAGTTAGCAAACAAGTATAACTTTATTGTGTCAAATGTGCCGTCA
  TACTCTCCGAATTCAATTGCGGAGTTTGCTGTGAACCAGGCTATTAATGTGGTTCGTCAT
  TTTAATCAAATTCAAACGAAAGTGCGGGAGCATGATTTTAGATGGGAACCTACTATTTTA
  TCGAAATCCATTAAAGATTTAAAAGTAGCTGTTATTGGTACAGGTCGAATTGGCCGTGTA
  GTAGCCGATATATTTGCCAATGGTTATCAAAGTGATGTGGTCGCATACGACCCGTTTCCT
  AATGCTAAAATTGCAACGTATGTCGATTACAAAGATACGATTGAGGAAGCGGTTGAAGGT
  GCTGATATCGTGACATTACATGTACCTGCAACGAAATATAATCATTATCTATTTAATGCT
  GAATTATTTAAACATTTTAAAAAGGGCGCAGTATTTGTCAATTGTGCGAGAGGTTCTTTA
  GTAGATACTAAGGCGTTATTAGACGCATTAGACAATGGTGTGATTAAAGGTGCAGCACTT
  GATACGTATGAATTTGAACGCAAACTTTTCCCAAGTGATCAAAGGGGCAAAACACTGAAC
  GATCCATTGTTAGAATCGTTGATTGACAGGGAAGATGTCATATTAACACCACATATTGCG
  TTTTATACTGAAGCTGCAGTTAAAAATCTAATTGTCGATGCATTAGATGCAACATTAGAT
  GTATTGCAGACTGGAGATACTAGGTTACGAGTAAATTAA
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⊟Protein

⊟General

• locus tag: NWMN_2459 [new locus tag: NWMN_RS14130 ]
• symbol: NWMN_2459
• description: D-lactate dehydrogenase
• length: 332
• theoretical pI: 5.25933
• theoretical MW: 37222.9
• GRAVY: -0.196386

⊟Function

• reaction:
  EC 1.1.1.28?  ExPASy
  D-lactate dehydrogenase (R)-lactate + NAD⁺ = pyruvate + NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 167.6)
  and 6 more
  Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 18)
  2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 16.7)
  glycerol-3-phosphate dehydrogenase (NAD(+)) (TIGR03376; EC 1.1.1.8; HMM-score: 14)
  Metabolism Energy metabolism Sugars inositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 14)
  glutamate synthase, small subunit (TIGR01318; HMM-score: 12.6)
  Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 12.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 172.2)
  and 13 more
  Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 100.5)
  NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 29)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 23)
  NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 21.2)
  IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 20.9)
  HUP (CL0039) UDPG_MGDP_dh_C; UDP-glucose/GDP-mannose dehydrogenase family, UDP binding domain (PF03720; HMM-score: 18.7)
  NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 18.1)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 17.8)
  AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 16.5)
  Form_Glyc_dh (CL0325) DpaA_N; Dipicolinate synthase subunit A N-terminal domain (PF16924; HMM-score: 14.8)
  NADP_Rossmann (CL0063) GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 14.7)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 14)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008111
  • TAT(Tat/SPI): 0.000323
  • LIPO(Sec/SPII): 0.000661
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222671 NCBI
• RefSeq: YP_001333493 NCBI
• UniProt: A0A0H3KAJ0 UniProt

⊟Protein sequence

• MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVAGYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYSPNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRVVADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLFNAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRGKTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2459 < NWMN_2460

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications