From AureoWiki
Revision as of 10:12, 11 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "* <aureodatabase>protein Genbank</aureodatabase> " to "")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07845 [old locus tag: SA1387 ]
  • pan locus tag?: SAUPAN004139000
  • symbol: SA_RS07845
  • pan gene symbol?: cshB
  • synonym:
  • product: DEAD/DEAH box family ATP-dependent RNA helicase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07845 [old locus tag: SA1387 ]
  • symbol: SA_RS07845
  • product: DEAD/DEAH box family ATP-dependent RNA helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 1592010..1593356
  • length: 1347
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1592010..1593356) NCBI
  • BioCyc: SA_RS07845 BioCyc
  • MicrobesOnline: see SA1387

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAAAACATCCATTCGAACAATTTAATCTAGAATCTAGTTTAATTGACGCTGTGAAA
    GACCTTAATTTTGAAAAACCAACTGAAATTCAGAATCGAATTATTCCAAGAATACTAAAG
    AGAACAAATTTAATTGGTCAATCTCAAACGGGTACAGGGAAATCTCATGCATTTTTATTA
    CCATTAATGCAGTTAATTGATAGTGAAATAAAAGAACCACAAGCAATCGTAGTTGCACCA
    ACAAGAGAACTTGCACAACAACTATACGATGCAGCGAACCATTTAAGCCAATTTAAAGCT
    GGTGTTTCAGTTAAAGTTTTTATTGGTGGTACAGATATAGAGAAAGATAGACAACGTTGT
    AATGCACAACCACAATTGATTATAGGCACCCCTACTAGAATTAATGACTTAGCTAAAACG
    GGACATTTACATGTGCACTTAGCATCATATTTAGTTATTGATGAAGCGGATCTTATGATT
    GACTTAGGATTAATTGAAGATGTAGATTACATTGCTGCAAGATTGGAAGATAATGCAAAT
    ATTGCGGTGTTTAGTGCTACAATTCCACAACAGTTACAACCATTTTTAAATAAATATTTA
    AGTCATCCAGAATATGTAGCTGTCGACAGTAAAAAACAAAATAAAAAGAACATCGAATTC
    TTTTTAATACCTACTAAAGGTGCAGCTAAAGTTGAAAAAACTTTAAATTTAATTGATATA
    CTAAATCCATACTTATGTATTATTTTCTGTAATAGTAGAGATAATGCAAATGATTTAGCA
    CGTTCACTAAATGAAGCTGGTATTAAAGTTGGTATGATTCATGGTGGTTTAACGCCACGT
    GAACGTAAACAACAAATGAAACGTATACGTAATTTAGAATTCCAATACGTTATTGCCAGC
    GATTTAGCATCTCGTGGTATTGATATTGAAGGTGTTAGTCATGTCATCAATTTTGATGTG
    CCAAATGATATTGACTTCTTTACGCATAGAGTTGGACGAACTGGTCGTGGGAATTATAAA
    GGTGTAGCAATTACGCTTTATAGTCCTGATGAAGAACACAATATTTCATTAATAGAAGAT
    CGCGGTTTTGTATTCAATACTGTTGATATTAAAGATGGTGAGTTAAAAGAAGTTAAAGCG
    CACAATCAGCGTCAAGCAAGAATGCGCAAAGATGACCATTTAACTAATCAAGTGAAGGAC
    AAAGTTCGAAGTAAAATTAAAAACAAAGTTAAACCAGGTTATAAGAAGAAATTTAAACAA
    GAAGTTGAAAAAATGAAACGTCAAGAGCGTAAACAATTTAGTAAGCAGCAAAATAGACAA
    AAACGTAAGCAAAACAAAAAAGGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS07845 [old locus tag: SA1387 ]
  • symbol: SA_RS07845
  • description: DEAD/DEAH box family ATP-dependent RNA helicase
  • length: 448
  • theoretical pI: 10.0841
  • theoretical MW: 51065.3
  • GRAVY: -0.556027

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 3.6.4.13?  ExPASy
    RNA helicase ATP + H2O = ADP + phosphate
  • ⊞TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 79.9)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 68.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 65.4)
    and 7 more
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 47.7)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 40.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 30.2)
    CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 18.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 17.3)
    CRISPR type AFERR-associated DEAD/DEAH-box helicase Csf4 (TIGR03117; HMM-score: 13.9)
    probable extracellular repeat, HAF family (TIGR02913; HMM-score: 12.1)
  • TheSEED: see SA1387
  • ⊞PFAM:
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 141)
    and 2 more
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 99.3)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 29.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9928
    • Cytoplasmic Membrane Score: 0.0031
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0037
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00254
    • TAT(Tat/SPI): 0.001049
    • LIPO(Sec/SPII): 0.000231
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNLIGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGVSVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMIDLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNIEFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGGLTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRTGRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDDHLTNQVKDKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS07845&oldid=94821"