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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1836 [new locus tag: SACOL_RS09420 ]
  • pan locus tag?: SAUPAN004500000
  • symbol: SACOL1836
  • pan gene symbol?: smdA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1836 [new locus tag: SACOL_RS09420 ]
  • symbol: SACOL1836
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1890534..1891442
  • length: 909
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTTATCTTCACCGATAGTCATTGGACTCATTATTGCAGTAGTCATTGCATTAATC
    TTTTTTGTCCTTTTTTTAGTTGCCTTAGGTAGTAAGAAAAAAGTTAAGCGACAAACAGAA
    GAAAAGTATGAACAACAAGAACAAAACATTAAAAAGTCTCATGAAGAAGCTTTAGAGAAA
    GAGCGTATTCAAAATAAAAAGACAATTACAAAACAACAAGAAGATTATAATCACATGGTT
    AGTACAAAAGATCGTGAGATTGACGCATTAAAATTGTTCTCGAAAAATCATAGTGAATAT
    GTAACGGACATGAGATTAATTGGTATTCGTGAAAGATTAGTTAAAGAAAAACGAATTCGA
    CCAGAAGATATGCACATTATGGCAAATATCTTTTTACCAAAGGATGGCTTTAATAATATT
    GAACGAATTAGTCATTTAGTATTAACAAGAACTGGTCTTTATATTATTGATTCTCAATTG
    TTAAAAGGTCATGTTTATAATGGTATTAGTGGCGGTCAATTTAAAGATTTACCACCAATG
    GAACAAGTATTTGATACATTGGATTTAGATAAATCTAGACCGCAAACTATTGTGATGGAT
    CAAAATGATGACAAACGTTCATTATCATTTGTAAATTATTCAGATCAAATTGAGGCGATT
    AAACAATTGGCTGAAGACTTACAAAAAGAATTAGGTGCCAAATATACACCAACTTCAATT
    TTATACTTTAATCTTAAAAATGAAGGCGATGTAACGATTTCAAATTATAATCAAAATAGT
    GCTGTCAAAGTGCTTGTCGGTGCTGAACAATTAGACGAGTTCTTTAACAAATTTGTATTC
    CATGGTCGTATTCAATACAACGTGGAAGACTTACAACAAATGATGGATAAAATTGAATCA
    TTCAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    909

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1836 [new locus tag: SACOL_RS09420 ]
  • symbol: SACOL1836
  • description: hypothetical protein
  • length: 302
  • theoretical pI: 6.41787
  • theoretical MW: 35052.9
  • GRAVY: -0.457616

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 15.3)
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.3)
    and 5 more
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 12)
    Cellular processes Cellular processes Sporulation and germination transcription factor, RsfA family (TIGR02894; HMM-score: 11.5)
    Signal transduction Regulatory functions DNA interactions transcription factor, RsfA family (TIGR02894; HMM-score: 11.5)
    Unknown function General TIGR00374 family protein (TIGR00374; HMM-score: 11.3)
    Metabolism Energy metabolism Methanogenesis tetrahydromethanopterin S-methyltransferase, subunit B (TIGR04166; EC 2.1.1.86; HMM-score: 7)
  • TheSEED  :
    • Hypothetical protein SAV1789
  • ⊞PFAM:
    PDDEXK (CL0236) NERD; Nuclease-related domain (PF08378; HMM-score: 25.3)
    no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 25.3)
    FlaX; FlaX (PF24151; HMM-score: 22.8)
    and 30 more
    Oxa1 (CL0376) EMC3_TMCO1; Integral membrane protein EMC3/TMCO1-like (PF01956; HMM-score: 17.6)
    MFS (CL0015) CLN3; CLN3 protein (PF02487; HMM-score: 16.2)
    no clan defined Tmemb_9; TMEM9 (PF05434; HMM-score: 15.6)
    FAM176; FAM176 family (PF14851; HMM-score: 15.6)
    IT (CL0182) ArsP_1; Predicted permease (PF03773; HMM-score: 15.2)
    no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 15.2)
    TPR (CL0020) DUF6377; Domain of unknown function (DUF6377) (PF19904; HMM-score: 14.9)
    no clan defined DUF6779; Domain of unknown function (DUF6779) (PF20570; HMM-score: 14.5)
    Polo-box (CL0708) C5orf34_2nd; C5orf34-like, second domain (PF22833; HMM-score: 14.3)
    FixH-like (CL0488) FixH; FixH (PF05751; HMM-score: 13.9)
    no clan defined PFF1_TM; Vacuolar membrane protease, transmembrane domain (PF22251; HMM-score: 13.7)
    DMT (CL0184) CSG2; Ceramide synthase regulator (PF16965; HMM-score: 13.6)
    no clan defined DUF7394; Family of unknown function (DUF7394) (PF24126; HMM-score: 13.6)
    HTH (CL0123) WH_AprA; AprA winged helix domain (PF23589; HMM-score: 13.5)
    no clan defined EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 13.3)
    DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 13.3)
    DUF7807; Family of unknown function (DUF7807) (PF25093; HMM-score: 13.3)
    Cadherin_C_2; Cadherin cytoplasmic C-terminal (PF16492; HMM-score: 13.1)
    DUF7261; Family of unknown function (DUF7261) (PF23922; HMM-score: 13)
    Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 12.6)
    FraC; Filament integrity protein FraC (PF24301; HMM-score: 12.5)
    LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 12.4)
    DUF3169; Protein of unknown function (DUF3169) (PF11368; HMM-score: 12.3)
    Transporter (CL0375) Connexin; Connexin (PF00029; HMM-score: 11.8)
    no clan defined DUF4006; Family of unknown function (DUF4006) (PF13179; HMM-score: 11.7)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 10.8)
    no clan defined DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 10.8)
    Piezo_TM1-24; Piezo TM1-24 (PF24871; HMM-score: 10.7)
    MtrB; Tetrahydromethanopterin S-methyltransferase subunit B (PF05440; HMM-score: 7.4)
    O-anti_assembly (CL0499) O-antigen_lig; O-antigen ligase like membrane protein (PF13425; HMM-score: 7.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0005
    • Cytoplasmic Membrane Score: 0.9635
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0345
  • ⊞LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017489
    • TAT(Tat/SPI): 0.000786
    • LIPO(Sec/SPII): 0.130279
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYEQQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVTDMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQLLKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIEAIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFNKFVFHGRIQYNVEDLQQMMDKIESFN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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