PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS07985 [old locus tag: SACOL1568 ]
pan locus tag^?: SAUPAN004078000
symbol: SACOL_RS07985
pan gene symbol^?: xseA
• synonym:
• product: exodeoxyribonuclease 7 large subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07985 [old locus tag: SACOL1568 ]
• symbol: SACOL_RS07985
• product: exodeoxyribonuclease 7 large subunit
• replicon: chromosome
• strand: -
• coordinates: 1603767..1605104
• length: 1338
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1603767..1605104) NCBI
• BioCyc: SACOL_RS07985 BioCyc
• MicrobesOnline: see SACOL1568

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTCAGATTATTTAAGTGTTTCAGCTTTAACGAAATATATTAAATATAAATTTGATCAA
  GATCCACATCTCCAATCTGTATTGATTAAAGGTGAGCTTTCAAATTTCAAAAAGCATTCA
  AGTGGTCATTTATACTTTAATGTGAAAGATAAAGAAAGCGTTATAAGTGCCATGATGTTC
  AAAGGTAGTGCTTCTAAATTAAACTTCGAACCTAAAGAAGGAGATGAAGTCTTATTAGAA
  GCACGTGTTTCTGTTTTTGAACGTCGTGGAAACTATCAAATTTATGTAAATAAAATGCAA
  TTAGATGGTATAGGGAACTTATATCAAAAATTAGAAGCATTAAAGAAAAAATTAACTGAA
  GAAGGTTGTTTTGATAAAGCGAATAAGAAATCAATACCTAAGTTTCCTAAAAAAATTGCA
  GTTTTAACAGCGAGTACAGGTGCCGCAATTCGAGATATCCATTCAACGATAAATAGTCGT
  TTTCCATTAGCTGAACAAATACAAATTAGTACTTTGGTTCAAGGTGAAAAAGCAAAAGAC
  GACATTATTGAAAAAATTGAATATGCAGATAGTTTAGGTGTAGATACCATTATTGTAGGT
  CGAGGCGGTGGATCCATAGAAGATTTATGGAATTTCAACGAAGAAGCTGTCGTCCGTGCA
  ATATATAATTGTAAGACACCGATTATATCAGCAGTTGGTCATGAAACAGACTTTACATTA
  AGTGATTTTGCTGCAGACATCAGAGCTGCGACTCCAACTCAAGCTGCTGTTATTGCAACA
  CCAGACCAATATGAATTGCTGCAACAAATACAGCAATATCAGTTTACATTGACTCGTTTC
  ATCAAGAAACATTTAGAACAACAACGTAAACATGTTGAACATTTGTCATCGTATTACAAG
  TTTAAACAACCAACTTTATTGTATGATCAACAGATACAACGTCGAGATGATTTAGAAAAG
  AGACTGAAACAACAAATTCAGGCAACTTTTGAACAACAGAGACATCGATTAATGCTGTTG
  CAACAACGTTATAATTTAAAAGCATTACTAAGTAGTGTTAATCAAGAACAACAAAATAAT
  CTTCAATTGACAAACCAACTTGTTAAATTATTGAATTCTAAGATATTAAGTTATAAAAAT
  GATTTGAAAAATAAAGTAGAAAACTTAAACAATTTAAGTCCAACTAATACAATGTTGCGT
  GGATATGCAATTGTTAATAAAAAAGACGAAGTCATTACGAGTACTAAAGATTTAACAGAA
  AATGATCAATTGACGTTAACAATGAAAGATGGCTTAGTAGATGCAAAAGTTACGAAAGTA
  AGGTGTAATAATGACTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS07985 [old locus tag: SACOL1568 ]
• symbol: SACOL_RS07985
• description: exodeoxyribonuclease 7 large subunit
• length: 445
• theoretical pI: 9.48419
• theoretical MW: 50893.8
• GRAVY: -0.52809

⊟Function

• ⊞reaction:
  EC 3.1.11.6?  ExPASy
  Exodeoxyribonuclease VII Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates
• TIGRFAM:
  Genetic information processing DNA metabolism Degradation of DNA exodeoxyribonuclease VII, large subunit (TIGR00237; EC 3.1.11.6; HMM-score: 489)
• TheSEED: see SACOL1568
• ⊞PFAM:
  no clan defined Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 340)
  and 8 more
  OB (CL0021) tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 102.9)
  tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 45.8)
  Cache (CL0165) dCache_1; Cache domain (PF02743; HMM-score: 15.1)
  OB (CL0021) RecJ_OB; RecJ OB domain (PF17768; HMM-score: 14.5)
  no clan defined DUF4279; Domain of unknown function (DUF4279) (PF14106; HMM-score: 11.8)
  NUC; Nuclease domain (PF18510; HMM-score: 11.8)
  PepSY_TM-like (CL0490) PepSY_TM_like_2; Putative PepSY_TM-like (PF16357; HMM-score: 11.6)
  no clan defined CHASE6_C; C-terminal domain of two-partite extracellular sensor domain (PF17150; HMM-score: 8.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8304
  • Cytoplasmic Membrane Score: 0.1268
  • Cell wall & surface Score: 0.0327
  • Extracellular Score: 0.01
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002793
  • TAT(Tat/SPI): 0.000164
  • LIPO(Sec/SPII): 0.000268
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447209672 NCBI
• RefSeq: WP_001286928 NCBI
• UniProt: see SACOL1568

⊟Protein sequence

• MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND

⊟Experimental data

• experimentally validated: see SACOL1568
• protein localization: see SACOL1568
• quantitative data / protein copy number per cell: see SACOL1568
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications