PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12705 [old locus tag: SA2214 ]
• pan locus tag^?: SAUPAN005966000
• symbol: SA_RS12705
• pan gene symbol^?: bioA
• synonym:
• product: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12705 [old locus tag: SA2214 ]
• symbol: SA_RS12705
• product: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
• replicon: chromosome
• strand: -
• coordinates: 2484683..2486041
• length: 1359
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2484683..2486041) NCBI
• BioCyc: SA_RS12705 BioCyc
• MicrobesOnline: see SA2214

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATTACACACAACAACTTAAACAAAAAGACTCAGAATATGTTTGGCATCCATTTACA
  CAAATGGGTGTATATAGCAAAGAAGAAGCAATCATCATTGAAAAAGGAAAGGGTAGTTAC
  CTTTACGATACGAATGGCAATAAATATTTAGATGGTTATGCATCGTTGTGGGTCAATGTG
  CATGGTCATAATAACAAATACTTGAATAAGGTAATTAAAAAGCAACTCAATAAAATTGCC
  CATTCTACGCTGCTAGGATCATCAAATATTCCGTCAATAGAACTTGCGGAAAAATTAATC
  GAAATCACGCCAAGTAATCTAAGAAAAGTATTTTATTCTGATACAGGCAGTGCGTCTGTT
  GAAATCGCAATAAAGATGGCATATCAGTATTGGAAAAATATTGATAGAGAAAAATATGCC
  AAGAAAAACAAGTTTATAACGCTAAATCACGGTTATCATGGGGATACGATTGGTGCGGTA
  AGTGTTGGTGGTATCAAGACCTTTCATAAAATATTTAAAGACTTAATATTTGAGAATATT
  CAAGTAGAAAGCCCATCTTTCTATCGCAGTAATTACGATACTGAAAATGAAATTATGACA
  GCTATTTTAACGAATATAGAGCAAATTCTAATTGAAAGAAATGATGAAATCGCAGGGTTT
  ATATTGGAACCGTTGATTCAAGGTGCGACAGGCTTGTTTGTTCATCCTAAAGGCTTTTTG
  AAAGAAGTCGAGAAATTGTGCAAAAAATACGATGTCTTATTAATTTGTGATGAGGTAGCA
  GTTGGTTTTGGGAGAACTGGAAAGATGTTTGCATGCAATCATGAAGATGTTCAACCGGAT
  ATTATGTGTTTAGGTAAGGCGATTACTGGTGGCTACTTACCACTTGCAGCTACATTGACA
  TCTCAAAAAATATACAATACATTTTTAAGTGATTCGCATGGTGTTAATACCTTTTTCCAT
  GGTCATACATACACCGGAAATCAAATCGTTTGTACGGTTGCATTAGAAAATATAAGACTT
  TATGAAAAACGTAAGTTATTGTCACATATTGAAACGACATCATCAACACTTGAGAAACAG
  TTACATGCGCTGAAGCGTCATCGAAATGTTGGTGATGTAAGAGGACGAGGCTTAATGTTT
  GGAGTTGAATTAGTTACAGATAAAGATAGTAAAACGCCGTTAGAAATTGAAAAAGTTGAA
  CGTATTGTACGTAAATGTAAAGAAAACGGGCTAATGATTAGAAATTTAGAAAATGTCATT
  ACGTTTGTGCCAGTGTTAAGTATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTT
  AAAAAGGCAGTACATAACATTTTAGATAGGAAGTGTTAA
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⊟Protein

⊟General

• locus tag: SA_RS12705 [old locus tag: SA2214 ]
• symbol: SA_RS12705
• description: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
• length: 452
• theoretical pI: 9.20767
• theoretical MW: 51430
• GRAVY: -0.287168

⊟Function

• reaction:
  EC 2.6.1.62?  ExPASy
  Adenosylmethionine--8-amino-7-oxononanoate transaminase S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 551.2)
  and 11 more
  transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 269.2)
  ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 229.8)
  Metabolism Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 203.5)
  Metabolism Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 200.5)
  Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 190.8)
  Metabolism Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 188.8)
  Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 168.3)
  L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 143.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 128.4)
  Metabolism Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 99.2)
  Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 12.5)
• TheSEED: see SA2214
• PFAM:
  PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 385.9)
  and 1 more
  no clan defined Pox_L5; Poxvirus L5 protein family (PF04872; HMM-score: 13.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.046625
  • TAT(Tat/SPI): 0.000523
  • LIPO(Sec/SPII): 0.00274
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447032805 NCBI
• RefSeq: WP_001110061 NCBI
• UniProt: see SA2214

⊟Protein sequence

• MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSQKIYNTFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: BirA* see SA2214

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications