PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10445 [old locus tag: SA1819 ]
• pan locus tag^?: SAUPAN005245000
• symbol: SA_RS10445
• pan gene symbol^?: tst
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10445 [old locus tag: SA1819 ]
• symbol: SA_RS10445
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2061076..2061780
• length: 705
• essential: no DEG

⊟Accession numbers

• Location: NC_002745 (2061076..2061780) NCBI
• BioCyc: SA_RS10445 BioCyc
• MicrobesOnline: see SA1819

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATAAAAAATTACTAATGAATTTTTTTATCGTAAGCCCTTTGTTGCTTGCGACAATC
  GCTACAGATTTTACCCCTGTTCCCTTATCATCTAATCAAATAATCAAAACTGCAAAAGCA
  TCTACAAACGATAATATAAAGGATTTGCTAGACTGGTATAGTAGTGGGTCTGACACTTTT
  ACAAATAGTGAAGTTTTAGATAATTCCTTAGGATCTATGCGTATAAAAAACACAGATGGC
  AGCATCAGCCTTATAATTTTTCCGAGTCCTTATTATAGCCCTGCTTTTACAAAAGGGGAA
  AAAGTTGACTTAAACACAAAAAGAACTAAAAAAAGCCAACATACTAGCGAAGGAACTTAT
  ATCCATTTCCAAATAAGTGGCGTTACAAATACTGAAAAATTACCTACTCCAATAGAACTA
  CCTTTAAAAGTTAAGGTTCATGGTAAAGATAGCCCCTTAAAGTATTGGCCAAAGTTCGAT
  AAAAAACAATTAGCTATATCAACTTTAGACTTTGAAATTCGTCATCAGCTAACTCAAATA
  CATGGATTATATCGTTCAAGCGATAAAACGGGTGGTTATTGGAAAATAACAATGAATGAC
  GGATCCACATATCAAAGTGATTTATCTAAAAAGTTTGAATACAATACTGAAAAACCACCT
  ATAAATATTGATGAAATAAAAACTATAGAAGCAGAAATTAATTAA
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⊟Protein

• 3MFG
  PDB

Protein Data Bank: 3MFG

⊟General

• locus tag: SA_RS10445 [old locus tag: SA1819 ]
• symbol: SA_RS10445
• description: hypothetical protein
• length: 234
• theoretical pI: 9.33755
• theoretical MW: 26446.9
• GRAVY: -0.508547

⊟Function

• TIGRFAM:
• TheSEED: see SA1819
• PFAM:
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 23.7)
  and 2 more
  GBD (CL0202) DUF4465; Domain of unknown function (DUF4465) (PF14717; HMM-score: 16.8)
  no clan defined DIRP; DIRP (PF06584; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 40 aa
  • SP(Sec/SPI): 0.740254
  • TAT(Tat/SPI): 0.010302
  • LIPO(Sec/SPII): 0.202086
  • Cleavage Site: CS pos: 40-41. AKA-ST. Pr: 0.3170
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446958343 NCBI
• RefSeq: WP_001035599 NCBI
• UniProt: see SA1819

⊟Protein sequence

• MNKKLLMNFFIVSPLLLATIATDFTPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYWPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEIN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, SaeR see SA1819

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications