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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1819 [new locus tag: SA_RS10445 ]
  • pan locus tag?: SAUPAN005245000
  • symbol: tst
  • pan gene symbol?: tst
  • synonym:
  • product: toxic shock syndrome toxin-1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1819 [new locus tag: SA_RS10445 ]
  • symbol: tst
  • product: toxic shock syndrome toxin-1
  • replicon: chromosome
  • strand: +
  • coordinates: 2061076..2061780
  • length: 705
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAAATTACTAATGAATTTTTTTATCGTAAGCCCTTTGTTGCTTGCGACAATC
    GCTACAGATTTTACCCCTGTTCCCTTATCATCTAATCAAATAATCAAAACTGCAAAAGCA
    TCTACAAACGATAATATAAAGGATTTGCTAGACTGGTATAGTAGTGGGTCTGACACTTTT
    ACAAATAGTGAAGTTTTAGATAATTCCTTAGGATCTATGCGTATAAAAAACACAGATGGC
    AGCATCAGCCTTATAATTTTTCCGAGTCCTTATTATAGCCCTGCTTTTACAAAAGGGGAA
    AAAGTTGACTTAAACACAAAAAGAACTAAAAAAAGCCAACATACTAGCGAAGGAACTTAT
    ATCCATTTCCAAATAAGTGGCGTTACAAATACTGAAAAATTACCTACTCCAATAGAACTA
    CCTTTAAAAGTTAAGGTTCATGGTAAAGATAGCCCCTTAAAGTATTGGCCAAAGTTCGAT
    AAAAAACAATTAGCTATATCAACTTTAGACTTTGAAATTCGTCATCAGCTAACTCAAATA
    CATGGATTATATCGTTCAAGCGATAAAACGGGTGGTTATTGGAAAATAACAATGAATGAC
    GGATCCACATATCAAAGTGATTTATCTAAAAAGTTTGAATACAATACTGAAAAACCACCT
    ATAAATATTGATGAAATAAAAACTATAGAAGCAGAAATTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

Protein Data Bank: 3MFG

⊟General[edit | edit source]

  • locus tag: SA1819 [new locus tag: SA_RS10445 ]
  • symbol: Tst
  • description: toxic shock syndrome toxin-1
  • length: 234
  • theoretical pI: 9.33755
  • theoretical MW: 26446.9
  • GRAVY: -0.508547

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Toxic shock syndrome toxin 1 (TSST-1)
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Toxic shock syndrome toxin 1 (TSST-1)
  • PFAM:
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 23.7)
    and 2 more
    GBD (CL0202) DUF4465; Domain of unknown function (DUF4465) (PF14717; HMM-score: 16.8)
    no clan defined DIRP; DIRP (PF06584; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: KTAKASTN
  • SignalP: Signal peptide SP(Sec/SPI) length 40 aa
    • SP(Sec/SPI): 0.740254
    • TAT(Tat/SPI): 0.010302
    • LIPO(Sec/SPII): 0.202086
    • Cleavage Site: CS pos: 40-41. AKA-ST. Pr: 0.3170
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKKLLMNFFIVSPLLLATIATDFTPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYWPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEIN

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, SaeR (activation) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Beenu Moza, Ashok K Varma, Rebecca A Buonpane, Penny Zhu, Christine A Herfst, Melissa J Nicholson, Anne-Kathrin Wilbuer, Nilufer P Seth, Kai W Wucherpfennig, John K McCormick, David M Kranz, Eric J Sundberg
Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1.
EMBO J: 2007, 26(4);1187-97
[PubMed:17268555] [WorldCat.org] [DOI] (P p)

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