PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10050 [old locus tag: SA1749 ]
• pan locus tag^?: SAUPAN005004000
• symbol: SA_RS10050
• pan gene symbol^?: aspB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10050 [old locus tag: SA1749 ]
• symbol: SA_RS10050
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2001463..2002749
• length: 1287
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2001463..2002749) NCBI
• BioCyc: SA_RS10050 BioCyc
• MicrobesOnline: see SA1749

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
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  841
  901
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  1021
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  1141
  1201
  1261
  
  ATGAATCCTTTAGCCCAAAGCTTAAATGAACAACTTCAACAGTCAAATGCAACTGCCTTT
  ACAATGTTATCTGACTTAGGTCAAAATATGTTTTATCCAAAAGGCATTTTATCTCAATCT
  GCTGAAGCAAAGAGTACAACATATAATGCAACTATAGGTATGGCGACAAACAAAGACGGA
  AAAATGTTTGCATCATCTTTAGATGCAATGTTTAATGATTTAACTCCAGATGAAATATTC
  CCTTATGCGCCACCACAAGGCATCGAAGAATTACGTGATTTATGGCAACAAAAAATGTTG
  CGTGACAATCCAGAGCTATCAATCGACAACATGTCACTACCAATTGTTACGAATGCATTA
  ACACATGGTTTATCTTTAGTTGGCGATTTATTTGTAAATCAAGGTGACACTATCTTGTTA
  CCAGAGCATAATTGGGGTAATTACAAACTTGTTTTCAATACGAGAAATGGTGCAAACCTT
  CAAACATATCCTATCTTTGATAAAGACGGGCATTATACTACTGATTCACTTGTAGAAGCT
  TTACAATCATACAATAAAGATAAAGTCATTATGATTTTAAATTATCCTAATAATCCGACA
  GGTTACACACCTACGCATAAAGAAGTGACTACCATCGTCGATGCAATTAAAGCATTAGCT
  GATAAAGGTACAAAAGTTATAGCTGTTGTGGATGACGCATACTATGGTTTATTCTATGAA
  GATGTGTATACTCAATCATTATTTACTGCATTATCTAATTTAAATTCAAATGCAATATTA
  CCTGTTCGTTTAGATGGTGCAACAAAAGAATTTTTCGCATGGGGATTCCGTGTTGGGTTT
  ATGACATTTGGAACGTCTGATCAAACAACTAAAGAAGTATTAGAAGCCAAAGTAAAAGGT
  CTTATACGAAGTAACATTTCTAGTGGACCTCTTCCAACACAAAGCGCTGTTAAGCATGTA
  TTAAAAAATAATAAACAATTCGATAAAGAAATCGAACAAAATATTCAAACATTAAAAGAA
  CGCTATGAAGTCACTAAGGAAGTTGTCTATGCTGATCAATATCATTCACATTGGCAAGCC
  TATGACTTTAACTCTGGATACTTTATGGCAATAAAAGTTCATGATGTTGACCCTGAAGAA
  CTTCGTAAACATTTAATTGAAAAATATTCAATTGGTGTTATTGCACTTAATGCAACAGAT
  ATTCGTATTGCGTTTAGCTGTGTAGAAAAAGAAGATATCCCACATGTATTTGATTCAATT
  GCTAAAGCGATTGATGACTTAAGATAA
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  1287
  

⊟Protein

⊟General

• locus tag: SA_RS10050 [old locus tag: SA1749 ]
• symbol: SA_RS10050
• description: hypothetical protein
• length: 428
• theoretical pI: 4.88204
• theoretical MW: 48119
• GRAVY: -0.316355

⊟Function

• TIGRFAM:
  tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 64.4)
  Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 62.8)
  LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 56.3)
  and 9 more
  Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 43.3)
  putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 35.3)
  beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 32.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 26.2)
  Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 25.8)
  succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 24.8)
  LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 19.9)
  succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 13.5)
  [FeFe] hydrogenase, group B1/B3 (TIGR04105; EC 1.12.-.-; HMM-score: 10.5)
• TheSEED: see SA1749
• PFAM:
  PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 125.1)
  and 4 more
  Thioredoxin (CL0172) AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 16)
  no clan defined Spore_II_R; Stage II sporulation protein R (spore_II_R) (PF09551; HMM-score: 14.2)
  4HB_MCP (CL0457) 4HB_MCP_1; Four helix bundle sensory module for signal transduction (PF12729; HMM-score: 13.6)
  no clan defined NEMO; NF-kappa-B essential modulator NEMO (PF11577; HMM-score: 10.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024746
  • TAT(Tat/SPI): 0.01037
  • LIPO(Sec/SPII): 0.001727
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446991286 NCBI
• RefSeq: WP_001068542 NCBI
• UniProt: see SA1749

⊟Protein sequence

• MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAKSTTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRDNPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANLQTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKALADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGFRVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQNIQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIGVIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: LexA see SA1749

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications