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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS09055 [old locus tag: SA1608 ]
- pan locus tag?: SAUPAN004501000
- symbol: SA_RS09055
- pan gene symbol?: metK
- synonym:
- product: S-adenosylmethionine synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGTTAAATAACAAACGATTATTTACTTCAGAGTCTGTTACAGAAGGACACCCAGATAAA
ATCGCTGACCAAGTGTCAGATGCAATATTAGATGCTATTTTAAAAGACGACCCAAATGCA
CGTGTAGCTTGTGAAACAACTGTTACAACAGGTATGGCATTGATCGCCGGCGAAATTTCT
ACAACAACATATGTTGATATTCCGAAAGTTGTTAGAGAAACAATTAAAGAAATTGGATAC
ACAAGAGCAAAATATGGTTATGATTATGAAACAATGGCAATTTTAACTGCGATTGATGAA
CAATCACCTGACATTGCACAAGGCGTGGATAAAGCATTAGAGTATCGTGATAAAGATAGC
GAAGAAGAAATTGAAGCAACAGGTGCAGGTGACCAAGGTTTAATGTTTGGTTACGCAACG
AATGAAACAGAAACGTACATGCCTCTAGCAATTTATTTATCACATCAATTGGCAAAACGT
TTATCTGATGTGCGTAAAGATGGTACGTTAAATTATTTACGACCAGACGGTAAAGTTCAG
GTCACTGTTGAATATGATGAAAATGATAACCCAGTACGTATTGATACGATTGTCGTATCA
ACACAACATGCTGATGATGTGACACTTGAACAAATTCAAGAAGACATCAAAGCGCATGTC
ATTTATCCAACTGTTCCAGAAAACTTGATTAATGAACAAACTAAATTCTACATCAATCCT
ACAGGACGCTTTGTAATCGGTGGACCTCAAGGTGATGCAGGATTAACAGGACGTAAAATC
ATTGTTGATACGTATGGTGGCTATGCACGTCATGGTGGCGGATGCTTCAGTGGTAAGGAT
CCTACAAAAGTAGACCGCTCAGCTGCATATGCTGCACGTTATGTGGCTAAAAATATTGTT
GCAGCAGGTTTAGCAGATCAATGTGAAGTACAATTGGCATATGCGATTGGTGTTGCAGAA
CCAGTGTCAATTGCAATTGATACATTTGGAACAGGTAAAGTTTCTGAAGGACAACTTGTC
GAAGCAGTTAGAAAACACTTTGACCTAAGACCTGCAGGCATTATTAAAATGCTTGATTTG
AAACAACCGATTTACAAACAAACTGCTGCTTATGGTCATTTTGGACGTACAGATGTATTA
TTCCCATGGGAAAAATTAGACAAAGTGGAAGAATTAAAAGACGCAGTAAAATATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS09055 [old locus tag: SA1608 ]
- symbol: SA_RS09055
- description: S-adenosylmethionine synthase
- length: 398
- theoretical pI: 4.61692
- theoretical MW: 43790
- GRAVY: -0.327889
⊟Function[edit | edit source]
- reaction: EC 2.5.1.6? ExPASyMethionine adenosyltransferase ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
- TIGRFAM: Central intermediary metabolism Other methionine adenosyltransferase (TIGR01034; EC 2.5.1.6; HMM-score: 621.1)
- TheSEED: see SA1608
- PFAM: no clan defined S-AdoMet_synt_C; S-adenosylmethionine synthetase, C-terminal domain (PF02773; HMM-score: 238.1)and 2 moreS-AdoMet_synt_M; S-adenosylmethionine synthetase, central domain (PF02772; HMM-score: 162.2)S-AdoMet_synt_N; S-adenosylmethionine synthetase, N-terminal domain (PF00438; HMM-score: 145.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: K+, Mg2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 10
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014999
- TAT(Tat/SPI): 0.000791
- LIPO(Sec/SPII): 0.001932
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVKY
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SA_RS08625 universal stress protein UspA [2] (data from MRSA252) SA_RS11680 30S ribosomal protein S5 [2] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: S-box see SA1608
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
Mol Microbiol: 2002, 43(6);1387-400
[PubMed:11952893] [WorldCat.org] [DOI] (P p) - ↑ 2.0 2.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)