From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1730 [new locus tag: SAUSA300_RS09465 ]
  • pan locus tag?: SAUPAN004501000
  • symbol: metK
  • pan gene symbol?: metK
  • synonym:
  • product: S-adenosylmethionine synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1730 [new locus tag: SAUSA300_RS09465 ]
  • symbol: metK
  • product: S-adenosylmethionine synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1913878..1915071
  • length: 1194
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTAAATAACAAACGATTATTTACTTCAGAGTCTGTTACAGAAGGACACCCAGATAAA
    ATCGCTGACCAAGTGTCAGATGCAATATTAGATGCTATTTTAAAAGACGATCCAAATGCA
    CGTGTAGCTTGTGAAACAACTGTTACAACAGGTATGGCATTGATCGCCGGCGAAATTTCT
    ACAACAACATATGTTGATATTCCGAAAGTTGTTAGAGAAACAATTAAAGAAATTGGATAC
    ACAAGAGCAAAATATGGTTATGATTATGAAACAATGGCAATTTTAACTGCGATTGATGAA
    CAATCACCTGACATTGCACAAGGCGTGGATAAAGCATTAGAGTATCGTGATAAAGATAGC
    GAAGAAGAAATTGAAGCAACAGGTGCAGGTGACCAAGGTTTAATGTTTGGTTACGCAACG
    AATGAAACAGAGACGTATATGCCTCTAGCAATTTATTTATCACATCAATTGGCAAAACGT
    TTATCAGATGTGCGTAAAGATGGTACGTTAAATTATTTACGACCAGACGGTAAAGTTCAG
    GTCACTGTTGAATATGATGAAAATGATAACCCAGTACGTATTGATACGATTGTCGTATCA
    ACACAACATGCTGAGGATGTGACACTTGAACAAATTCAAGAAGACATTAAAGCACATGTC
    ATTTATCCAACTGTTCCAGAAAACTTGATTAATGAACAAACTAAATTCTACATCAATCCT
    ACAGGACGCTTTGTAATCGGTGGACCTCAAGGTGATGCAGGATTAACAGGACGTAAAATC
    ATTGTTGATACGTACGGTGGCTATGCACGTCATGGTGGCGGATGCTTCAGTGGTAAGGAT
    CCTACAAAAGTAGACCGTTCAGCTGCATACGCTGCACGTTATGTGGCTAAAAATATTGTT
    GCAGCAGGTTTAGCAGATCAATGTGAAGTACAATTGGCATATGCGATTGGTGTTGCAGAA
    CCAGTGTCAATTGCAATTGATACATTTGGAACAGGTAAAGTTTCTGAAGGGCAACTTGTC
    GAAGCAGTTAGAAAACACTTTGACCTAAGACCTGCAGGTATTATTAAAATGCTTGATTTG
    AAACAACCGATTTACAAACAAACTGCTGCTTATGGTCATTTTGGACGTACAGATGTCTTA
    TTCCCATGGGAAAAATTAGACAAAGTGGAAGAATTAAAAGACGCAGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1730 [new locus tag: SAUSA300_RS09465 ]
  • symbol: MetK
  • description: S-adenosylmethionine synthetase
  • length: 397
  • theoretical pI: 4.62015
  • theoretical MW: 43640.9
  • GRAVY: -0.325441

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.6?  ExPASy
    Methionine adenosyltransferase ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
  • TIGRFAM:
    Metabolism Central intermediary metabolism Other methionine adenosyltransferase (TIGR01034; EC 2.5.1.6; HMM-score: 621.4)
  • TheSEED  :
    • S-adenosylmethionine synthetase (EC 2.5.1.6)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  S-adenosylmethionine synthetase (EC 2.5.1.6)
    and 2 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  S-adenosylmethionine synthetase (EC 2.5.1.6)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  S-adenosylmethionine synthetase (EC 2.5.1.6)
  • PFAM:
    no clan defined S-AdoMet_synt_C; S-adenosylmethionine synthetase, C-terminal domain (PF02773; HMM-score: 238.2)
    and 2 more
    S-AdoMet_synt_M; S-adenosylmethionine synthetase, central domain (PF02772; HMM-score: 162.9)
    S-AdoMet_synt_N; S-adenosylmethionine synthetase, N-terminal domain (PF00438; HMM-score: 145.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: K+, Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014999
    • TAT(Tat/SPI): 0.000791
    • LIPO(Sec/SPII): 0.001932
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_1656universal stress protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: S-box (transcription termination) regulon
    S-box(RNA)important in Methionine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_1730&oldid=83658"