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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08820 [old locus tag: SA1563 ]
  • pan locus tag?: SAUPAN004409000
  • symbol: SA_RS08820
  • pan gene symbol?: —
  • synonym:
  • product: tRNA-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08820 [old locus tag: SA1563 ]
  • symbol: SA_RS08820
  • product: tRNA-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1794687..1795283
  • length: 597
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1794687..1795283) NCBI
  • BioCyc: SA_RS08820 BioCyc
  • MicrobesOnline: see SA1563

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
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    481
    541
    ATGAATTTATTTTACAATCCTAAATATGTAGGAGATGTCGCATTTTTACAAATTGAACCA
    GTTGAAGGTGAATTAAACTACAATAAAAAAGGTAATGTTGTTGAAATTACTAATGAAGGT
    AATGTTGTAGGTTATAATATTTTTGAAATTTCAAAAGATATAACAATTGAAGAAAAAGGT
    CATATTAAATTAACTGATGAACTTGTAAATGTATTCCAAAAGCGTATTTCAGAAGCTGGT
    TTTGATTATAAATTAAATGCTGATCTATCACCGAAATTTGTAGTTGGCTACGTTGAAACT
    AAAGACAAACATCCTGATGCAGATAAATTAAGTGTACTAAATGTAAACGTTGGAAATGAC
    ACATTACAAATTGTATGTGGCGCGCCTAACGTTGAAGCTGGACAGAAAGTTGTTGTTGCT
    AAAGTAGGTGCAGTGATGCCTAGCGGTATGGTAATTAAAGATGCTGAATTACGTGGTGTT
    GCCTCAAGCGGTATGATTTGTTCAATGAAAGAATTGAATTTACCTAATGCACCTGAAGAA
    AAAGGTATTATGGTATTAAATGACAGCTATGAAATTGGACAAGCATTTTTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    597

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08820 [old locus tag: SA1563 ]
  • symbol: SA_RS08820
  • description: tRNA-binding protein
  • length: 198
  • theoretical pI: 4.4561
  • theoretical MW: 21689.7
  • GRAVY: -0.113636

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.20?  ExPASy
    Phenylalanine--tRNA ligase ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00472; EC 6.1.1.20; HMM-score: 145)
    and 1 more
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase, beta subunit (TIGR00399; EC 6.1.1.10; HMM-score: 24.4)
  • TheSEED: see SA1563
  • PFAM:
    OB (CL0021) tRNA_bind; Putative tRNA binding domain (PF01588; HMM-score: 84.3)
    no clan defined DUF4479; Domain of unknown function (DUF4479) (PF14794; HMM-score: 76.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008349
    • TAT(Tat/SPI): 0.000172
    • LIPO(Sec/SPII): 0.000992
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVGYNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKDKHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGVASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS1176050S ribosomal protein L4  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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