PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08780 [old locus tag: SA1557 ]
• pan locus tag^?: SAUPAN004400000
• symbol: SA_RS08780
• pan gene symbol^?: ccpA
• synonym:
• product: catabolite control protein A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08780 [old locus tag: SA1557 ]
• symbol: SA_RS08780
• product: catabolite control protein A
• replicon: chromosome
• strand: -
• coordinates: 1784194..1785183
• length: 990
• essential: yes DEG other strains

⊟Accession numbers

• Location: NC_002745 (1784194..1785183) NCBI
• BioCyc: SA_RS08780 BioCyc
• MicrobesOnline: see SA1557

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACAGTTACTATATATGATGTAGCAAGAGAAGCGCGTGTCTCTATGGCCACAGTGTCG
  CGTGTTGTTAATGGGAACCAAAATGTTAAAGCAGAAACTAAAAATAAAGTTAACGAAGTC
  ATTAAGCGTTTGAATTATCGTCCAAATGCTGTTGCTAGAGGTTTAGCTAGTAAAAAGACA
  ACAACAGTAGGTGTGATCATTCCAGATATATCTAATATCTATTATTCACAACTTGCTCGT
  GGACTTGAAGATATTGCAACAATGTATAAATATCACTCAATTATTTCAAATTCAGATAAC
  GATCCTGAAAAGGAAAAAGAAATTTTTAATAACTTATTAAGTAAACAGGTTGATGGTATT
  ATTTTCCTTGGTGGTACAATTACTGAAGAAATGAAAGAATTGATAAATCAATCATCTGTA
  CCTGTAGTAGTATCAGGAACAAATGGTAAGGATGCACATATAGCATCAGTTAATATTGAT
  TTTACTGAAGCTGCGAAAGAAATTACGGGAGAATTAATTGAAAAAGGCGCTAAATCATTT
  GCTTTAGTAGGTGGAGAACATTCTAAAAAAGCTCAAGAAGATGTTTTAGAAGGTTTAACT
  GAAGTGTTAAATAAAAATGGCCTTCAATTAGGTGATACATTGAATTGTTCTGGTGCTGAA
  AGTTATAAAGAAGGCGTAAAAGCTTTTGCTAAAATGAAAGGCAATTTGCCAGATGCCATT
  TTATGTATCAGCGACGAAGAAGCAATTGGTATTATGCATAGTGCAATGGATGCTGGTATT
  AAAGTTCCAGAGGAATTACAAATTATTAGTTTCAATAATACACGATTAGTTGAGATGGTT
  AGACCACAACTTTCTAGTGTTATTCAACCATTATATGATATCGGTGCAGTAGGGATGCGC
  TTATTAACAAAATATATGAACGATGAAAAGATAGAAGAACCAAATGTAGTTTTACCTCAC
  AGAATTGAATACCGAGGAACTACAAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS08780 [old locus tag: SA1557 ]
• symbol: SA_RS08780
• description: catabolite control protein A
• length: 329
• theoretical pI: 5.50763
• theoretical MW: 36060
• GRAVY: -0.229787

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 570.3)
  and 5 more
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 159)
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 107.1)
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 18.6)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 18.6)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 18.6)
• TheSEED: see SA1557
• PFAM:
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 105.4)
  and 9 more
  Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 74.7)
  Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 71.1)
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 67.1)
  no clan defined ATP-synt_F; ATP synthase (F/14-kDa) subunit (PF01990; HMM-score: 16.2)
  4Fe-4S (CL0344) Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 14.6)
  MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 14.5)
  no clan defined DUF327; Protein of unknown function (DUF327) (PF03885; HMM-score: 13.9)
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 12.9)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by CcpA, TF important in Carbon catabolism: see SA1557

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.027924
  • TAT(Tat/SPI): 0.004638
  • LIPO(Sec/SPII): 0.00505
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446141211 NCBI
• RefSeq: WP_000219066 NCBI
• UniProt: see SA1557

⊟Protein sequence

• MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRLNYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDPEKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNIDFTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSGAESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRLVEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SA1557

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications